FastQCFastQC Report
Fri 10 Feb 2017
ERR1633490.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633490.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542952
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19600.3609895534043525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.2703738083661171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8570.1578408404426174No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5550.10221898068337532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT250.005493821629.59999827
CGGTAAT451.321956E-424.6666681
GGTATCA3700.024.01
GTATCAA9550.021.8900551
GGTACCG806.946211E-720.81256
TTTCGGG555.138291E-420.18181822
GTCTTAT609.228263E-418.51
ACGGTAC701.21769466E-418.53
ACCGTAC500.007030219318.49999827
TACCGCA1351.1477823E-917.8148167
CGTTAAA1054.785743E-717.61904717
GGCCGTT953.5993016E-617.52631614
CACGGTA752.0647365E-417.2666662
GTTTCGG650.001578482217.07692521
GCCGTTA1005.866308E-616.6515
ATATAGG904.4390075E-516.4444453
CCGTGCA803.378455E-416.187510
GGTTTCG803.378455E-416.187520
TAATACG700.002590132615.8571424
CGAACTA1059.324871E-615.85714229
Produced by FastQC (version 0.11.5)