##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633490.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	542952
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.213140388100605	33.0	33.0	33.0	27.0	33.0
2	31.47379878884321	33.0	33.0	33.0	27.0	33.0
3	31.225362462980154	33.0	33.0	33.0	27.0	33.0
4	31.551693335690818	33.0	33.0	33.0	27.0	33.0
5	31.65255308019862	33.0	33.0	33.0	27.0	33.0
6	34.45777711473574	37.0	37.0	37.0	27.0	37.0
7	34.63671926800159	37.0	37.0	37.0	27.0	37.0
8	34.77319910415654	37.0	37.0	37.0	27.0	37.0
9	34.9009544121764	37.0	37.0	37.0	27.0	37.0
10	34.89908500198913	37.0	37.0	37.0	27.0	37.0
11	34.90712254490268	37.0	37.0	37.0	27.0	37.0
12	34.95093304748855	37.0	37.0	37.0	27.0	37.0
13	34.943847706611265	37.0	37.0	37.0	27.0	37.0
14	34.96041823218259	37.0	37.0	37.0	27.0	37.0
15	34.96093024797772	37.0	37.0	37.0	27.0	37.0
16	34.93639032547997	37.0	37.0	37.0	27.0	37.0
17	34.93873859936053	37.0	37.0	37.0	27.0	37.0
18	34.9009286272083	37.0	37.0	37.0	27.0	37.0
19	34.89064226671971	37.0	37.0	37.0	27.0	37.0
20	34.89179706493392	37.0	37.0	37.0	27.0	37.0
21	34.88889809780607	37.0	37.0	37.0	27.0	37.0
22	34.783886973434115	37.0	37.0	37.0	27.0	37.0
23	34.81655468623377	37.0	37.0	37.0	27.0	37.0
24	34.811373749429045	37.0	37.0	37.0	27.0	37.0
25	34.827292283664114	37.0	37.0	37.0	27.0	37.0
26	34.70291849003227	37.0	37.0	37.0	27.0	37.0
27	34.69899180774728	37.0	37.0	37.0	27.0	37.0
28	34.72523906348996	37.0	37.0	37.0	27.0	37.0
29	34.73039605711002	37.0	37.0	37.0	27.0	37.0
30	34.730933857873254	37.0	37.0	37.0	27.0	37.0
31	34.72998902297072	37.0	37.0	37.0	27.0	37.0
32	34.717785365925536	37.0	37.0	37.0	27.0	37.0
33	34.70484499550605	37.0	37.0	37.0	27.0	37.0
34	34.67142583506461	37.0	37.0	37.0	27.0	37.0
35	34.61044438550737	37.0	37.0	37.0	27.0	37.0
36	34.64258903181128	37.0	37.0	37.0	27.0	37.0
37	34.64135503690934	37.0	37.0	37.0	27.0	37.0
38	34.63686476889301	37.0	37.0	37.0	27.0	37.0
39	34.53603265113675	37.0	37.0	37.0	27.0	37.0
40	34.32005591654511	37.0	37.0	37.0	27.0	37.0
41	34.50522882317406	37.0	37.0	37.0	27.0	37.0
42	34.52475541115973	37.0	37.0	37.0	27.0	37.0
43	34.12231099618383	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	27.0
17	23.0
18	33.0
19	40.0
20	134.0
21	309.0
22	671.0
23	1454.0
24	2639.0
25	4363.0
26	6619.0
27	9118.0
28	12086.0
29	15341.0
30	19308.0
31	24001.0
32	29627.0
33	38570.0
34	54898.0
35	102139.0
36	221539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.87212129248994	20.781026683758416	13.542817781314001	24.804034242437638
2	16.506799864444737	21.974502350115664	37.50386774521505	24.01483004022455
3	18.617115325111612	26.704754748117697	29.666710869469128	25.011419057301566
4	13.314068278595528	18.381551223680916	38.40873594719238	29.89564455053117
5	14.750291001782848	36.8387997465706	34.65923322872003	13.751676022926521
6	30.121999734783184	39.355965168191666	17.374648219363774	13.147386877661377
7	26.884328633102005	32.04463746334851	23.14661332861837	17.92442057493112
8	24.865549809191236	35.602226347817115	20.494261002814245	19.0379628401774
9	25.99216873683125	15.350896580176515	20.016686557927773	38.64024812506447
10	15.124541395924501	27.799142465632322	33.74441939618972	23.33189674225346
11	34.226782478009106	23.524915646318643	24.057006880902915	18.191294994769336
12	22.840324743255387	25.38051245782316	30.613571733781253	21.165591065140195
13	28.35922880843979	21.975607420177106	25.933784201918403	23.731379569464703
14	22.061618706626	20.98362286168943	27.64719533218406	29.307563099500513
15	24.085554524156834	28.724086107059186	23.9739424479512	23.21641692083278
16	23.175713506903005	28.26492949653008	25.06612002534294	23.493236971223975
17	22.634413355140047	27.638723128379674	26.429592302818666	23.297271213661613
18	23.30776937924531	26.854860098130224	27.54221367634708	22.295156846277386
19	24.459620739954914	26.4894502644801	27.25563217374648	21.795296821818503
20	24.26218155564396	26.15387732248891	27.236109269327674	22.34783185253945
21	23.244780385743123	26.763875996404842	27.435574415417936	22.5557692024341
22	23.05783936701587	26.62408463363244	27.193011536931444	23.12506446242025
23	23.116408080272286	26.395703487601114	27.514586924811034	22.973301507315565
24	23.186764207517424	26.972365881330205	26.75485125756973	23.086018653582638
25	23.435589149685423	26.610271257864415	27.312359103567168	22.641780488882997
26	23.40869911152367	26.734775818120205	27.120629447906996	22.735895622449128
27	23.359523493789506	26.853755028068782	26.895379333716424	22.891342144425288
28	22.980300284371364	26.671786824618017	27.330961116268103	23.01695177474252
29	23.80431419351987	26.594984455347802	26.708069958302023	22.892631392830307
30	23.301507315563807	26.68781034050892	27.175514594291943	22.83516774963533
31	23.546280334173186	26.287590799923382	27.066481014896343	23.099647851007088
32	22.78654466693188	26.513393449144672	27.35140491240478	23.34865697151866
33	22.69500803017578	26.58025018786191	27.586416478804754	23.138325303157554
34	23.203340258439052	26.254438698080122	27.34238017356967	23.199840869911153
35	23.192289557824633	26.959289218936483	26.981943155196042	22.86647806804285
36	23.688650193755617	26.517629551046866	26.893353371937113	22.900366883260396
37	23.617925709823336	26.18905538611148	26.994467282559047	23.198551621506137
38	23.27737995255566	26.632556837436827	26.82097128291267	23.269091927094845
39	22.8801072654673	26.513025092457525	27.19945777895652	23.407409863118655
40	23.030765150510543	26.934425142554037	27.317516097187227	22.71729360974819
41	23.195973124696106	26.17947811224565	27.27165568963739	23.352893073420855
42	22.87863383871871	27.010306620106384	27.12283958802988	22.988219953145027
43	23.327292283664118	26.171926800159127	27.136468785454333	23.36431213072242
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	23.0
2	31.0
3	107.5
4	184.0
5	184.0
6	252.5
7	321.0
8	298.5
9	276.0
10	400.5
11	525.0
12	525.0
13	822.5
14	1120.0
15	1968.5
16	2817.0
17	3257.0
18	3697.0
19	3697.0
20	3664.0
21	3631.0
22	4157.5
23	4684.0
24	6027.5
25	7371.0
26	7371.0
27	9237.0
28	11103.0
29	13456.5
30	15810.0
31	17970.0
32	20130.0
33	20130.0
34	23823.0
35	27516.0
36	29933.0
37	32350.0
38	34315.0
39	36280.0
40	36280.0
41	38109.0
42	39938.0
43	40880.5
44	41823.0
45	41791.5
46	41760.0
47	41760.0
48	41361.5
49	40963.0
50	40307.5
51	39652.0
52	38027.5
53	36403.0
54	36403.0
55	34869.5
56	33336.0
57	30199.5
58	27063.0
59	24900.0
60	22737.0
61	22737.0
62	20250.5
63	17764.0
64	14786.5
65	11809.0
66	9986.0
67	8163.0
68	8163.0
69	6885.5
70	5608.0
71	4515.0
72	3422.0
73	2757.0
74	2092.0
75	2092.0
76	1565.0
77	1038.0
78	817.5
79	597.0
80	487.0
81	377.0
82	377.0
83	315.0
84	253.0
85	213.0
86	173.0
87	124.5
88	76.0
89	76.0
90	55.5
91	35.0
92	21.5
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	542952.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.46053213712575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.47924766942354	59.88875846404154
2	7.466196388212781	10.222795555546666
3	2.008265409942369	4.124607559117127
4	0.8719338500302838	2.3877222144578414
5	0.5134379743434639	1.7575118471480733
6	0.3047394804330624	1.2517576196183207
7	0.22028531836213033	1.055659508194732
8	0.17275746465584044	0.9461654368799626
9	0.10658904004448601	0.6567428161287828
>10	0.7560773171183177	9.809090677982967
>50	0.06354054702088724	2.94425676039203
>100	0.035843377459563334	4.055184085851797
>500	5.4308147665998E-4	0.2624883070148654
>1k	5.4308147665998E-4	0.6372591476253248
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1960	0.3609895534043525	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1468	0.2703738083661171	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	857	0.1578408404426174	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	555	0.10221898068337532	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.8417834357364922E-4	0.0
4	0.0	0.0	0.0	1.8417834357364922E-4	0.0
5	0.0	0.0	1.8417834357364922E-4	1.8417834357364922E-4	0.0
6	0.0	0.0	7.367133742945969E-4	1.8417834357364922E-4	0.0
7	0.0	0.0	0.0011050700614418955	3.6835668714729845E-4	0.0
8	0.0	0.0	0.0012892484050155448	3.6835668714729845E-4	0.0
9	0.0	0.0	0.0018417834357364923	9.208917178682461E-4	0.0
10	0.0	0.0	0.0020259617793101418	0.0011050700614418955	0.0
11	0.0	0.0	0.004236101902193932	0.0014734267485891938	0.0
12	0.0	0.0	0.004604458589341231	0.0014734267485891938	0.0
13	0.0	0.0	0.004972815276488529	0.001657605092162843	0.0
14	1.8417834357364922E-4	0.0	0.005341171963635828	0.0018417834357364923	0.0
15	1.8417834357364922E-4	0.0	0.006262063681504074	0.0018417834357364923	0.0
16	1.8417834357364922E-4	0.0	0.006630420368651372	0.0018417834357364923	0.0
17	1.8417834357364922E-4	0.0	0.006814598712225022	0.0018417834357364923	0.0
18	1.8417834357364922E-4	0.0	0.006998777055798671	0.0020259617793101418	0.0
19	1.8417834357364922E-4	0.0	0.007367133742945969	0.002210140122883791	0.0
20	1.8417834357364922E-4	0.0	0.007367133742945969	0.0025784968100310895	0.0
21	1.8417834357364922E-4	0.0	0.007551312086519619	0.0025784968100310895	0.0
22	1.8417834357364922E-4	0.0	0.007735490430093268	0.0029468534971783876	0.0
23	1.8417834357364922E-4	0.0	0.007735490430093268	0.003315210184325686	0.0
24	1.8417834357364922E-4	0.0	0.007735490430093268	0.003867745215046634	0.0
25	1.8417834357364922E-4	0.0	0.007735490430093268	0.0040519235586202835	0.0
26	1.8417834357364922E-4	0.0	0.007735490430093268	0.004420280245767582	0.0
27	1.8417834357364922E-4	0.0	0.007735490430093268	0.005156993620062179	0.0
28	1.8417834357364922E-4	0.0	0.007735490430093268	0.015286802516612887	0.0
29	1.8417834357364922E-4	0.0	0.007735490430093268	0.03094196172037307	0.0
30	1.8417834357364922E-4	0.0	0.007735490430093268	0.05488514638494747	0.0
31	1.8417834357364922E-4	0.0	0.007735490430093268	0.11050700614418954	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGAAT	25	0.0054938216	29.599998	27
CGGTAAT	45	1.321956E-4	24.666668	1
GGTATCA	370	0.0	24.0	1
GTATCAA	955	0.0	21.890055	1
GGTACCG	80	6.946211E-7	20.8125	6
TTTCGGG	55	5.138291E-4	20.181818	22
GTCTTAT	60	9.228263E-4	18.5	1
ACGGTAC	70	1.21769466E-4	18.5	3
ACCGTAC	50	0.0070302193	18.499998	27
TACCGCA	135	1.1477823E-9	17.814816	7
CGTTAAA	105	4.785743E-7	17.619047	17
GGCCGTT	95	3.5993016E-6	17.526316	14
CACGGTA	75	2.0647365E-4	17.266666	2
GTTTCGG	65	0.0015784822	17.076925	21
GCCGTTA	100	5.866308E-6	16.65	15
ATATAGG	90	4.4390075E-5	16.444445	3
CCGTGCA	80	3.378455E-4	16.1875	10
GGTTTCG	80	3.378455E-4	16.1875	20
TAATACG	70	0.0025901326	15.857142	4
CGAACTA	105	9.324871E-6	15.857142	29
>>END_MODULE