FastQCFastQC Report
Fri 10 Feb 2017
ERR1633489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679291
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31080.45753587196061773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22110.3254864262885862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12520.18430981714758476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTA250.0054947829.615
GGTATCA6450.024.6666681
CGCGATA400.00193037923.12530
CGATTTT652.6801645E-622.7692321
GTATCAA16250.021.4030761
ACACGCA806.9515045E-720.812537
ACCGGCC450.003824220320.5555578
CGAATTA450.003824220320.55555715
CCGCACT450.003824220320.55555726
AGCGTTC500.007031991618.537
TAGACCC609.231515E-418.55
GCGTTTC701.2182655E-418.514
CGACAGG609.231515E-418.537
TCTCGAC609.231515E-418.534
CTCGACA609.231515E-418.535
TCGACAG701.2182655E-418.536
AACACGC701.2182655E-418.536
CGAACTA1950.018.02564229
CGGAGTC1552.0008883E-1117.90322727
ACAACGG1451.546141E-1017.86206823
Produced by FastQC (version 0.11.5)