##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633489.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	679291
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.830352529328373	33.0	33.0	33.0	27.0	33.0
2	31.94539011999276	33.0	33.0	33.0	33.0	33.0
3	31.66393195257997	33.0	33.0	33.0	27.0	33.0
4	31.94863909576308	33.0	33.0	33.0	33.0	33.0
5	32.05348223368188	33.0	33.0	33.0	33.0	33.0
6	35.237216450681665	37.0	37.0	37.0	33.0	37.0
7	35.36282094124609	37.0	37.0	37.0	33.0	37.0
8	35.498831870288285	37.0	37.0	37.0	33.0	37.0
9	35.620330609414815	37.0	37.0	37.0	33.0	37.0
10	35.620220200179304	37.0	37.0	37.0	33.0	37.0
11	35.65122016926472	37.0	37.0	37.0	33.0	37.0
12	35.63975527424918	37.0	37.0	37.0	33.0	37.0
13	35.63130381530154	37.0	37.0	37.0	33.0	37.0
14	35.61195570087047	37.0	37.0	37.0	33.0	37.0
15	35.62471753637248	37.0	37.0	37.0	33.0	37.0
16	35.59730954774905	37.0	37.0	37.0	33.0	37.0
17	35.61018473673286	37.0	37.0	37.0	33.0	37.0
18	35.582787052971405	37.0	37.0	37.0	33.0	37.0
19	35.584465273351185	37.0	37.0	37.0	33.0	37.0
20	35.58608460880536	37.0	37.0	37.0	33.0	37.0
21	35.58255151326898	37.0	37.0	37.0	33.0	37.0
22	35.49016548136218	37.0	37.0	37.0	33.0	37.0
23	35.53816847271641	37.0	37.0	37.0	33.0	37.0
24	35.528623226275634	37.0	37.0	37.0	33.0	37.0
25	35.54862937975036	37.0	37.0	37.0	33.0	37.0
26	35.43992044646551	37.0	37.0	37.0	33.0	37.0
27	35.44740030414064	37.0	37.0	37.0	33.0	37.0
28	35.46561193950752	37.0	37.0	37.0	33.0	37.0
29	35.480085854221535	37.0	37.0	37.0	33.0	37.0
30	35.471607897057375	37.0	37.0	37.0	33.0	37.0
31	35.46764346944093	37.0	37.0	37.0	33.0	37.0
32	35.45671884361783	37.0	37.0	37.0	33.0	37.0
33	35.445742693484824	37.0	37.0	37.0	33.0	37.0
34	35.41902660273727	37.0	37.0	37.0	33.0	37.0
35	35.374958596536686	37.0	37.0	37.0	33.0	37.0
36	35.39037761430668	37.0	37.0	37.0	33.0	37.0
37	35.3950560216461	37.0	37.0	37.0	33.0	37.0
38	35.38081470238823	37.0	37.0	37.0	33.0	37.0
39	35.2838047316982	37.0	37.0	37.0	33.0	37.0
40	35.074705832993516	37.0	37.0	37.0	33.0	37.0
41	35.24089675853206	37.0	37.0	37.0	33.0	37.0
42	35.277264088586485	37.0	37.0	37.0	33.0	37.0
43	34.950039084869374	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	15.0
16	25.0
17	32.0
18	21.0
19	27.0
20	32.0
21	121.0
22	326.0
23	696.0
24	1509.0
25	2716.0
26	4482.0
27	6839.0
28	9660.0
29	12813.0
30	16774.0
31	21381.0
32	27353.0
33	36051.0
34	52146.0
35	105342.0
36	380925.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.28819607502528	21.09758557083783	13.493627914987833	24.120590439149055
2	15.883178196089748	22.151625739189832	38.921905339537844	23.04329072518258
3	17.909997335457117	27.1700935239831	30.468532631817585	24.451376508742204
4	12.19536251768388	17.78354195771768	40.405952677129534	29.615142847468906
5	13.468896246233205	37.48746855176942	35.68190952036756	13.361725681629816
6	29.194262841698183	40.72790600788175	17.156564712325057	12.921266438095014
7	26.248397225931154	32.69232184733789	23.65216085595128	17.40712007077968
8	24.244837632178257	35.996207810790956	20.898112885346634	18.860841671684152
9	25.576078587821716	15.334223477125414	20.814496290985748	38.275201644067124
10	14.38529290098058	28.68888296768248	34.351257413980164	22.574566717356774
11	33.31341060016989	24.095564345766395	24.820732204607452	17.770292849456272
12	22.793765852926064	26.19113163577907	30.729981701509367	20.285120809785496
13	27.58640994801933	22.858539271092948	26.74936073052639	22.80569005036133
14	21.753858066719562	21.2789511417051	28.21721471357636	28.749976077998973
15	23.304887007188377	29.24137078218319	25.106618518425826	22.347123692202604
16	22.527458776871768	29.15083520906357	25.54074763245796	22.780958381606705
17	21.967757558984292	28.040265512129558	26.933523335359954	23.058453593526192
18	22.694544753279523	27.54460165083889	28.268297386539786	21.4925562093418
19	24.203323759625846	26.60067629337059	28.01847808965524	21.177521857348324
20	23.415149030386093	27.120188549531793	28.066322091710326	21.398340328371788
21	22.485944904319357	27.421237731693783	28.401524530723947	21.691292833262917
22	22.328133303694585	27.382668105421683	28.167456951439075	22.121741639444657
23	22.291771862132723	27.132112746967056	28.519883231192523	22.056232159707694
24	22.33946865187379	27.766008971118417	27.171418434809237	22.72310394219856
25	22.532316783234286	27.526788960842996	27.999487701147224	21.941406554775494
26	22.750779857233496	27.303909517423314	27.92838415347767	22.01692647186552
27	22.439720237718443	27.332615918656362	27.66958490543817	22.55807893818702
28	22.04018601747999	27.04599354326791	28.616896146128834	22.296924293123272
29	22.995152298499466	26.977539817250634	27.708890593280344	22.318417290969556
30	22.397470303595952	27.259745823218623	28.191010921681574	22.151772951503848
31	22.543504919099473	27.29080762147592	28.032610471800744	22.133076987623863
32	21.798021760924257	27.227800751077226	28.220011747542657	22.75416574045586
33	21.878841321318845	27.12357443275415	28.955484468364812	22.042099777562193
34	22.592968256608728	26.67384081343636	28.36089393205563	22.37229699789928
35	22.08685232102295	27.76350636178015	28.236646739026426	21.912994578170476
36	23.17740114325083	26.984164371381336	27.89629186902226	21.94214261634557
37	22.461360447878747	26.825322284558457	28.097972739223692	22.615344528339108
38	22.509646086875875	27.296548901722534	27.88981452720557	22.30399048419602
39	22.329458214520727	27.183048207616473	28.029224588578384	22.458268989284417
40	21.934929212958806	27.835198758705772	28.316583025536918	21.913289002798507
41	22.49463043084628	26.74347223796576	28.052631346506875	22.70926598468109
42	22.164580422823207	27.508387421591042	28.101505834760065	22.225526320825683
43	22.710885320135258	26.696069872852725	27.993599208586602	22.599445598425415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	49.0
2	77.0
3	229.5
4	382.0
5	382.0
6	471.0
7	560.0
8	492.0
9	424.0
10	602.5
11	781.0
12	781.0
13	1294.5
14	1808.0
15	3077.0
16	4346.0
17	5172.0
18	5998.0
19	5998.0
20	6151.0
21	6304.0
22	7054.5
23	7805.0
24	9654.5
25	11504.0
26	11504.0
27	14276.0
28	17048.0
29	19852.0
30	22656.0
31	26052.5
32	29449.0
33	29449.0
34	34104.5
35	38760.0
36	41773.5
37	44787.0
38	46684.0
39	48581.0
40	48581.0
41	49912.0
42	51243.0
43	52636.5
44	54030.0
45	52914.5
46	51799.0
47	51799.0
48	49498.0
49	47197.0
50	47569.0
51	47941.0
52	45495.0
53	43049.0
54	43049.0
55	40231.0
56	37413.0
57	32767.0
58	28121.0
59	26168.0
60	24215.0
61	24215.0
62	21761.5
63	19308.0
64	16080.5
65	12853.0
66	10555.0
67	8257.0
68	8257.0
69	6639.0
70	5021.0
71	4099.5
72	3178.0
73	2476.5
74	1775.0
75	1775.0
76	1379.5
77	984.0
78	773.5
79	563.0
80	460.0
81	357.0
82	357.0
83	305.0
84	253.0
85	241.0
86	229.0
87	185.0
88	141.0
89	141.0
90	96.5
91	52.0
92	35.0
93	18.0
94	10.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	679291.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.53207736009575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.72198478673491	52.494618919316835
2	7.544883678242685	9.134149649686199
3	1.999483513415827	3.6309867214296867
4	0.8931268592614052	2.1625129654876294
5	0.5996591996450757	1.8149308531304413
6	0.36310761348397497	1.3187794889671032
7	0.25553956890692164	1.082783866754751
8	0.19868142606646197	0.9621279562135374
9	0.15330725720256896	0.8352006077565
>10	1.0818165807667781	12.857139898698295
>50	0.12273681206800804	5.1418474399537475
>100	0.0629670871782285	6.890975696593123
>500	0.001967721474304331	0.6956039680733548
>1k	7.378955528641242E-4	0.9783419679387874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3108	0.45753587196061773	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2211	0.3254864262885862	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1252	0.18430981714758476	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.4721231401564278E-4	2.9442462803128556E-4	0.0	0.0
4	0.0	1.4721231401564278E-4	2.9442462803128556E-4	0.0	0.0
5	0.0	1.4721231401564278E-4	2.9442462803128556E-4	1.4721231401564278E-4	0.0
6	0.0	1.4721231401564278E-4	0.0011776985121251422	2.9442462803128556E-4	0.0
7	0.0	1.4721231401564278E-4	0.0022081847102346417	2.9442462803128556E-4	0.0
8	0.0	1.4721231401564278E-4	0.00264982165228157	2.9442462803128556E-4	0.0
9	0.0	1.4721231401564278E-4	0.0035330955363754267	5.888492560625711E-4	0.0
10	0.0	1.4721231401564278E-4	0.0038275201644067123	5.888492560625711E-4	0.0
11	0.0	1.4721231401564278E-4	0.00529964330456314	5.888492560625711E-4	0.0
12	0.0	1.4721231401564278E-4	0.005594067932594426	5.888492560625711E-4	0.0
13	0.0	1.4721231401564278E-4	0.005594067932594426	5.888492560625711E-4	0.0
14	0.0	1.4721231401564278E-4	0.005741280246610069	5.888492560625711E-4	0.0
15	0.0	1.4721231401564278E-4	0.0066245541307039255	5.888492560625711E-4	0.0
16	0.0	1.4721231401564278E-4	0.007360615700782139	5.888492560625711E-4	0.0
17	0.0	1.4721231401564278E-4	0.0076550403288134245	7.360615700782139E-4	0.0
18	0.0	1.4721231401564278E-4	0.0076550403288134245	8.832738840938567E-4	0.0
19	0.0	1.4721231401564278E-4	0.007949464956844711	8.832738840938567E-4	0.0
20	0.0	1.4721231401564278E-4	0.007949464956844711	8.832738840938567E-4	0.0
21	0.0	1.4721231401564278E-4	0.008391101898891638	0.001324910826140785	0.0
22	0.0	1.4721231401564278E-4	0.008538314212907282	0.0019137600822033561	0.0
23	0.0	1.4721231401564278E-4	0.008538314212907282	0.002060972396218999	0.0
24	0.0	1.4721231401564278E-4	0.008538314212907282	0.002060972396218999	0.0
25	0.0	1.4721231401564278E-4	0.008538314212907282	0.002060972396218999	0.0
26	0.0	1.4721231401564278E-4	0.008538314212907282	0.0023553970242502845	0.0
27	0.0	1.4721231401564278E-4	0.008538314212907282	0.003385883222359784	0.0
28	0.0	1.4721231401564278E-4	0.008538314212907282	0.013249108261407851	0.0
29	0.0	1.4721231401564278E-4	0.008538314212907282	0.0275287027209252	0.0
30	0.0	1.4721231401564278E-4	0.008538314212907282	0.05461576849980347	0.0
31	0.0	1.4721231401564278E-4	0.008538314212907282	0.10952596162763822	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCGTA	25	0.00549478	29.6	15
GGTATCA	645	0.0	24.666668	1
CGCGATA	40	0.001930379	23.125	30
CGATTTT	65	2.6801645E-6	22.76923	21
GTATCAA	1625	0.0	21.403076	1
ACACGCA	80	6.9515045E-7	20.8125	37
ACCGGCC	45	0.0038242203	20.555557	8
CGAATTA	45	0.0038242203	20.555557	15
CCGCACT	45	0.0038242203	20.555557	26
AGCGTTC	50	0.0070319916	18.5	37
TAGACCC	60	9.231515E-4	18.5	5
GCGTTTC	70	1.2182655E-4	18.5	14
CGACAGG	60	9.231515E-4	18.5	37
TCTCGAC	60	9.231515E-4	18.5	34
CTCGACA	60	9.231515E-4	18.5	35
TCGACAG	70	1.2182655E-4	18.5	36
AACACGC	70	1.2182655E-4	18.5	36
CGAACTA	195	0.0	18.025642	29
CGGAGTC	155	2.0008883E-11	17.903227	27
ACAACGG	145	1.546141E-10	17.862068	23
>>END_MODULE