FastQCFastQC Report
Fri 10 Feb 2017
ERR1633488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748077
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78181.0450795840535132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59790.7992492751414627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29550.3950128128521529No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC21630.289141358443048No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC20940.27991770900589114No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG20090.26855524230794425No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18510.24743442185764297No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA17940.23981488536607862No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC17680.23633930731729486No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG17160.2293881512197274No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC16920.2261799253285424No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT16720.22350640375255487No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG16180.21628789549738864No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG15920.21281231744860488No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15790.211074528424213No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG15350.20519278095704052No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15080.2015835268294574No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG15030.20091514643546055No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT14690.19637015975628178No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA14420.19276090562869863No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14070.18808224287072053No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG13810.1846066648219368No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC13410.1792596216699618No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC13070.17471463499078305No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA12960.17324419812398992No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT12540.16762980281441617No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT12090.1616143792684443No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT12040.16094599887444744No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG11860.15853982945605866No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG11430.15279175806768555No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT11410.15252440591008679No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11090.14824677138850678No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG10940.14624163020651618No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA10880.1454395737337199No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC10790.14423648902452554No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA10720.14330075647292992No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT10650.1423650239213343No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC10470.13995885450294557No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10400.13902312195134992No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT10300.13768636116335617No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA10250.1370179807693593No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC10120.13528019174496742No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG9830.13140358545978556No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT9780.13073520506578867No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT9720.12993314859299243No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG9510.12712595093820556No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT9460.1264575705442087No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9400.12565551407141243No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9380.1253881619138137No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC9380.1253881619138137No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC9200.12298199249542494No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA9190.12284831641662555No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC8960.11977376660423994No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT8560.11442672345226494No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA8540.1141593712946662No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC8490.11349099090066932No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT8260.1104164410882837No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8200.10961438461548745No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8180.1093470324578887No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG8120.10854497598509244No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA8110.10841129990629306No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC8080.10801027166989494No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG8060.1077429195122962No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA7960.10640615872430244No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG7900.10560410225150621No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTAC7860.1050693979363087No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA7850.10493572185750932No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG7800.10426734146351245No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7790.10413366538471307No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGC7760.10373263714831495No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT7740.10346528499071621No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT7680.10266322851791995No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7590.10146014380872556No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA7590.10146014380872556No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG7530.10065808733592933No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG7510.10039073517833057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCACG303.5982876E-430.83333222
ACTAGTG303.5982876E-430.8333328
TCAATCC303.5982876E-430.83333221
TTAGACT250.00549513329.5999984
TCGTATG250.00549513329.59999837
AGCCTTA250.00549513329.5999986
TTTTGAC250.00549513329.59999831
GGTCGCT405.9374004E-527.7528
AATCCTC358.866487E-426.42857223
CTCGTAT358.866487E-426.42857236
GCTCTCG358.866487E-426.42857218
TAGGCGG358.866487E-426.42857234
CTCTCGC358.866487E-426.42857219
TTGCTCT451.3226083E-424.66666816
GTCCGCT451.3226083E-424.66666810
CGCTCTC1750.023.25714329
GCGTTAG400.001930560523.1258
GCCACGC400.001930560523.12523
TAGCTCC400.001930560523.12512
GCCGCTC1700.022.85294227
Produced by FastQC (version 0.11.5)