FastQCFastQC Report
Fri 10 Feb 2017
ERR1633487.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633487.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563428
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24460.434128229338975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18340.3255074295207196No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG13350.23694243097609632No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12810.2273582427568385No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12430.22061381400995334No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10370.18405191080315497No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10210.18121215133078228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9990.17730748205626984No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9730.1726928729136642No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9230.16381862456249957No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9040.16044641018905698No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT7960.14127803375054135No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT7850.1393256991132851No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7850.1393256991132851No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT7740.13737336447602888No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7710.136840909574959No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7690.13648593964091243No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC7400.13133887559723692No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7400.13133887559723692No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7260.1288540860589108No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7180.12743420632272448No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7170.12725672135570118No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6950.12335205208118873No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6820.1210447475098859No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6740.11962486777369957No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG6740.11962486777369957No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT6660.11820498803751323No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG6430.11412283379597747No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA6260.1111055893565815No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA5760.10223134100541684No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC5690.10098894623625379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC200.001841139637.03
ACACCGC250.00549399529.68
TTACGCT250.00549399529.64
GCTATAC358.863759E-426.4285723
CGCACCT400.001929971723.12529
TTGTCCG652.6788166E-622.7692313
GTCAACC2700.022.61111315
GTATCAA13650.022.2271041
TCGCCGT502.6997336E-422.225
GGTATCA5050.021.6138611
TCAACCC2950.020.69491416
TCGCACC450.003823423520.55555528
CTGAGCG450.003823423520.55555534
AGGCGGA450.003823423520.55555519
CGGAATT555.1386224E-420.18181827
GTCGCCG555.1386224E-420.18181824
CCGTCCG555.1386224E-420.18181828
CGGGATA555.1386224E-420.18181817
GCCGTCC656.894152E-519.92307727
ACGGGCT951.6723243E-719.47368416
Produced by FastQC (version 0.11.5)