##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633487.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563428
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.784992936098313	33.0	33.0	33.0	27.0	33.0
2	31.903050611613196	33.0	33.0	33.0	33.0	33.0
3	31.628126397694114	33.0	33.0	33.0	27.0	33.0
4	31.92306204164507	33.0	33.0	33.0	33.0	33.0
5	32.01987831630661	33.0	33.0	33.0	33.0	33.0
6	35.17730393235693	37.0	37.0	37.0	33.0	37.0
7	35.32487380818845	37.0	37.0	37.0	33.0	37.0
8	35.44322610874859	37.0	37.0	37.0	33.0	37.0
9	35.560978510120194	37.0	37.0	37.0	33.0	37.0
10	35.57171280092576	37.0	37.0	37.0	33.0	37.0
11	35.59427291508409	37.0	37.0	37.0	33.0	37.0
12	35.5785317733588	37.0	37.0	37.0	33.0	37.0
13	35.57512938654096	37.0	37.0	37.0	33.0	37.0
14	35.55821329433397	37.0	37.0	37.0	33.0	37.0
15	35.56405432459871	37.0	37.0	37.0	33.0	37.0
16	35.53745110289159	37.0	37.0	37.0	33.0	37.0
17	35.55609057412837	37.0	37.0	37.0	33.0	37.0
18	35.53045287064186	37.0	37.0	37.0	33.0	37.0
19	35.5307421711381	37.0	37.0	37.0	33.0	37.0
20	35.51732963218016	37.0	37.0	37.0	33.0	37.0
21	35.5410575974215	37.0	37.0	37.0	33.0	37.0
22	35.43654912428917	37.0	37.0	37.0	33.0	37.0
23	35.49268761935864	37.0	37.0	37.0	33.0	37.0
24	35.477571224717266	37.0	37.0	37.0	33.0	37.0
25	35.48728675181212	37.0	37.0	37.0	33.0	37.0
26	35.37598237929247	37.0	37.0	37.0	33.0	37.0
27	35.386574682124426	37.0	37.0	37.0	33.0	37.0
28	35.422600580730816	37.0	37.0	37.0	33.0	37.0
29	35.41591649687272	37.0	37.0	37.0	33.0	37.0
30	35.414525014731254	37.0	37.0	37.0	33.0	37.0
31	35.39771541350448	37.0	37.0	37.0	33.0	37.0
32	35.39914416748901	37.0	37.0	37.0	33.0	37.0
33	35.39865075928069	37.0	37.0	37.0	33.0	37.0
34	35.37992432041006	37.0	37.0	37.0	33.0	37.0
35	35.32168440333104	37.0	37.0	37.0	33.0	37.0
36	35.337129145161406	37.0	37.0	37.0	33.0	37.0
37	35.33502062375317	37.0	37.0	37.0	33.0	37.0
38	35.32526604996557	37.0	37.0	37.0	33.0	37.0
39	35.23282477974116	37.0	37.0	37.0	33.0	37.0
40	35.021001796147864	37.0	37.0	37.0	27.0	37.0
41	35.185862257466795	37.0	37.0	37.0	33.0	37.0
42	35.22779485577571	37.0	37.0	37.0	33.0	37.0
43	34.89898443101869	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	14.0
16	33.0
17	28.0
18	19.0
19	20.0
20	43.0
21	116.0
22	275.0
23	669.0
24	1343.0
25	2425.0
26	3976.0
27	5894.0
28	8242.0
29	11084.0
30	14390.0
31	18497.0
32	23446.0
33	30637.0
34	43772.0
35	88674.0
36	309826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.55034538574583	21.38889086094408	13.549734837459267	24.511028915850826
2	16.165685766415585	21.3113299303549	39.12407619074664	23.39890811248287
3	17.06482460935559	28.14964822479536	29.944731181268946	24.840795984580105
4	11.983962458379775	18.042944262620956	40.68505647571651	29.288036803282765
5	13.062183633046281	38.0364483128279	36.05766841548521	12.843699638640608
6	28.667371873602303	41.05120796268556	17.365839113427093	12.915581050285041
7	26.36201963693675	32.29037250544879	24.149669522991402	17.197938334623057
8	23.94733666058485	36.39843955216993	20.55790624534102	19.096317541904202
9	25.68278466813861	15.526384915197683	20.601922517162798	38.18890789950091
10	15.042383410125163	27.95281739636653	34.16177399774239	22.843025195765918
11	33.27807634693342	24.139375394904054	24.355729569705446	18.22681868845709
12	23.112979830608346	26.51927131771939	29.522316959753507	20.845431891918754
13	26.66516396061254	22.866098241478948	26.448099845943045	24.02063795196547
14	21.624590897151013	21.2564870755447	28.218334907033377	28.900587120270917
15	23.95940563834243	28.839177321680854	25.361359392859423	21.840057647117288
16	22.643354607864712	28.696301923227104	25.42205925158139	23.238284217326793
17	21.920990792079913	28.00482049170435	26.44526008647067	23.62892862974506
18	22.088004146048828	28.017954379264076	28.405049092341876	21.488992382345216
19	24.575633443847305	26.3776383140348	27.73255855229062	21.314169689827274
20	23.26508444734731	26.762603207508324	28.29571835265553	21.676593992488836
21	22.521599920486736	27.56661010812384	28.06285807592097	21.848931895468453
22	22.223425175887602	27.395337114946365	27.76486081628886	22.616376892877174
23	22.19201033672448	27.311386725544345	28.6627572644597	21.833845673271473
24	22.86698566631406	28.22561179068133	26.045918910668263	22.86148363233634
25	22.544672966199762	27.588795729001752	27.946072967619646	21.920458337178843
26	22.683998665313048	27.836564742966267	28.02789353741738	21.4515430543033
27	22.668202503247976	27.653932711899305	26.766862846716883	22.91100193813584
28	21.710316136223263	27.216787238120933	28.81681421583592	22.25608240981989
29	24.319167666498647	26.906543515764213	27.06148789197555	21.712800925761588
30	23.210064107570087	26.601979312352242	28.052918917767666	22.135037662310005
31	22.353344171748653	27.06202034687662	28.217802452132307	22.36683302924242
32	21.310974960420854	26.869094187722297	28.491129301348177	23.328801550508672
33	21.479230709158934	27.07604165927146	28.832077922999922	22.612649708569684
34	22.882604343412112	26.841406532866667	28.52911108429116	21.746878039430058
35	22.78055048737372	27.690139645172053	27.477690139645173	22.051619727809054
36	23.81369048041631	27.344753899344727	26.849215871415694	21.992339748823277
37	22.381919251439403	26.583875845715866	27.574951901573936	23.459253001270792
38	22.83592579708499	27.23080855051577	27.3552255123991	22.57804014000014
39	21.912826483596838	27.309789360841137	28.030023357021662	22.747360798540363
40	22.356716386122095	28.113263806555583	28.032863116494035	21.497156690828287
41	22.01736512917356	26.609611165934243	28.207508324044955	23.165515380847243
42	21.67996620686228	28.11734596079712	27.75243686859723	22.450250963743372
43	23.06630128428122	25.983266717309046	27.768765485563375	23.18166651284636
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	42.0
2	51.0
3	166.5
4	282.0
5	282.0
6	330.0
7	378.0
8	364.0
9	350.0
10	460.5
11	571.0
12	571.0
13	942.0
14	1313.0
15	2356.5
16	3400.0
17	3861.5
18	4323.0
19	4323.0
20	4045.0
21	3767.0
22	4092.5
23	4418.0
24	5784.0
25	7150.0
26	7150.0
27	9316.0
28	11482.0
29	13496.0
30	15510.0
31	18879.5
32	22249.0
33	22249.0
34	27974.0
35	33699.0
36	36636.5
37	39574.0
38	42526.0
39	45478.0
40	45478.0
41	47045.0
42	48612.0
43	49506.0
44	50400.0
45	47762.5
46	45125.0
47	45125.0
48	42171.0
49	39217.0
50	39102.5
51	38988.0
52	36424.5
53	33861.0
54	33861.0
55	33764.5
56	33668.0
57	27785.5
58	21903.0
59	19905.0
60	17907.0
61	17907.0
62	15964.0
63	14021.0
64	11142.5
65	8264.0
66	6863.5
67	5463.0
68	5463.0
69	4512.0
70	3561.0
71	3048.5
72	2536.0
73	2565.0
74	2594.0
75	2594.0
76	2206.0
77	1818.0
78	1345.0
79	872.0
80	561.5
81	251.0
82	251.0
83	198.5
84	146.0
85	128.0
86	110.0
87	80.5
88	51.0
89	51.0
90	37.0
91	23.0
92	15.5
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	563428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.339040581416775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.35171910270279	42.60510973083355
2	7.317239176710043	7.22051121367259
3	1.9952809589730125	2.9533574461829293
4	0.925514776323805	1.8265604443096433
5	0.5800842436594184	1.4310400019276248
6	0.3752851422402593	1.110972531755695
7	0.2789188186475563	0.9633110838520824
8	0.22675464063129083	0.8950285132905469
9	0.1748089447882738	0.7762415058812958
>10	1.4221515480748934	14.097047241450708
>50	0.19040143097949278	6.6131723296672025
>100	0.14682879575560714	13.464994074811399
>500	0.012449324328405596	4.200482702070758
>1k	0.0025630961852599753	1.8421711802939884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2446	0.434128229338975	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1834	0.3255074295207196	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1335	0.23694243097609632	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1281	0.2273582427568385	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1243	0.22061381400995334	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1037	0.18405191080315497	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1021	0.18121215133078228	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	999	0.17730748205626984	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	973	0.1726928729136642	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	923	0.16381862456249957	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	904	0.16044641018905698	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	796	0.14127803375054135	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	785	0.1393256991132851	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	785	0.1393256991132851	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	774	0.13737336447602888	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	771	0.136840909574959	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	769	0.13648593964091243	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	740	0.13133887559723692	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	740	0.13133887559723692	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	726	0.1288540860589108	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	718	0.12743420632272448	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	717	0.12725672135570118	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	695	0.12335205208118873	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	682	0.1210447475098859	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	674	0.11962486777369957	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	674	0.11962486777369957	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	666	0.11820498803751323	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	643	0.11412283379597747	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	626	0.1111055893565815	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	576	0.10223134100541684	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	569	0.10098894623625379	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.7748496702329312E-4	0.0	0.0
2	1.7748496702329312E-4	0.0	1.7748496702329312E-4	0.0	0.0
3	1.7748496702329312E-4	0.0	1.7748496702329312E-4	0.0	0.0
4	1.7748496702329312E-4	0.0	3.5496993404658623E-4	0.0	0.0
5	1.7748496702329312E-4	0.0	8.874248351164656E-4	0.0	0.0
6	1.7748496702329312E-4	0.0	0.001419879736186345	0.0	0.0
7	1.7748496702329312E-4	0.0	0.0024847895383261036	1.7748496702329312E-4	0.0
8	1.7748496702329312E-4	0.0	0.002662274505349397	1.7748496702329312E-4	0.0
9	1.7748496702329312E-4	0.0	0.0030172444393959832	3.5496993404658623E-4	0.0
10	7.099398680931725E-4	0.0	0.0035496993404658624	3.5496993404658623E-4	0.0
11	7.099398680931725E-4	0.0	0.004792094109628915	3.5496993404658623E-4	0.0
12	7.099398680931725E-4	0.0	0.0051470640436755005	3.5496993404658623E-4	0.0
13	7.099398680931725E-4	0.0	0.005324549010698794	3.5496993404658623E-4	0.0
14	7.099398680931725E-4	0.0	0.0060344888787919665	5.324549010698794E-4	0.0
15	7.099398680931725E-4	0.0	0.006566943779861846	5.324549010698794E-4	0.0
16	7.099398680931725E-4	0.0	0.007099398680931725	7.099398680931725E-4	0.0
17	8.874248351164656E-4	0.0	0.007276883647955018	7.099398680931725E-4	0.0
18	8.874248351164656E-4	0.0	0.007454368614978312	7.099398680931725E-4	0.0
19	8.874248351164656E-4	0.0	0.007631853582001604	8.874248351164656E-4	0.0
20	8.874248351164656E-4	0.0	0.0078093385490248975	0.0010649098021397589	0.0
21	8.874248351164656E-4	0.0	0.008164308483071484	0.0010649098021397589	0.0
22	8.874248351164656E-4	0.0	0.008164308483071484	0.001419879736186345	0.0
23	8.874248351164656E-4	0.0	0.008164308483071484	0.0021298196042795177	0.0
24	8.874248351164656E-4	0.0	0.008341793450094778	0.003194729406419276	0.0
25	8.874248351164656E-4	0.0	0.008341793450094778	0.0035496993404658624	0.0
26	8.874248351164656E-4	0.0	0.008341793450094778	0.0035496993404658624	0.0
27	8.874248351164656E-4	0.0	0.008341793450094778	0.0051470640436755005	0.0
28	8.874248351164656E-4	0.0	0.008341793450094778	0.01721604180125943	0.0
29	8.874248351164656E-4	0.0	0.008341793450094778	0.03780429797596144	0.0
30	0.0010649098021397589	0.0	0.008341793450094778	0.06282967832624577	0.0
31	0.0010649098021397589	0.0	0.008341793450094778	0.11962486777369957	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	20	0.0018411396	37.0	3
ACACCGC	25	0.005493995	29.6	8
TTACGCT	25	0.005493995	29.6	4
GCTATAC	35	8.863759E-4	26.428572	3
CGCACCT	40	0.0019299717	23.125	29
TTGTCCG	65	2.6788166E-6	22.76923	13
GTCAACC	270	0.0	22.611113	15
GTATCAA	1365	0.0	22.227104	1
TCGCCGT	50	2.6997336E-4	22.2	25
GGTATCA	505	0.0	21.613861	1
TCAACCC	295	0.0	20.694914	16
TCGCACC	45	0.0038234235	20.555555	28
CTGAGCG	45	0.0038234235	20.555555	34
AGGCGGA	45	0.0038234235	20.555555	19
CGGAATT	55	5.1386224E-4	20.181818	27
GTCGCCG	55	5.1386224E-4	20.181818	24
CCGTCCG	55	5.1386224E-4	20.181818	28
CGGGATA	55	5.1386224E-4	20.181818	17
GCCGTCC	65	6.894152E-5	19.923077	27
ACGGGCT	95	1.6723243E-7	19.473684	16
>>END_MODULE