FastQCFastQC Report
Fri 10 Feb 2017
ERR1633486.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633486.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751887
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT200042.6605061664851237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT153152.036875221941595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79671.0596007112770933No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA43310.5760174068709792No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38210.5081880654938841No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG35300.46948544129636505No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA33740.44873764275748884No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG27360.3638844666818285No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT26660.35457455708105073No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA26330.3501855996978269No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC23290.3097539922887349No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.2890061937498587No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT20990.27916428931475074No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC20250.26932238487964283No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA19820.2636034404105936No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC18890.25123456051241744No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT18330.24378663283179522No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17610.23421072581385236No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC17330.23048676197354123No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA16890.2246348187959095No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT16120.21439391823505394No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG16020.21306393114922853No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15440.20535000605144121No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG14820.19710408611932376No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC14820.19710408611932376No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC14680.19524210419916824No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT14410.19165113906743966No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA14360.19098614552452695No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC14280.18992215585586664No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA14130.18792717522712854No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC14010.18633119072413806No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG13740.1827402255924095No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13670.1818092346323317No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT13590.18074524496367142No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT13390.1780852707920206No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13370.17781927337485554No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC13340.17742027724910792No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA13150.17489330178603965No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12890.17143533536289363No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12420.16518439605951427No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12370.16451940251660155No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG12310.16372141026510634No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG12210.16239142317928093No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG12090.16079543867629045No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11730.15600748516731905No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG11640.15481049679007616No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11490.15281551616133807No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11430.15201752390984283No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG11330.15068753682401745No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11260.14975654586393966No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA11260.14975654586393966No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG11160.14842655877811425No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG11110.14776156523520156No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC11010.14643157814937618No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT10950.14563358589788092No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10920.1452345897721333No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10870.1445695962292206No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG10840.144170600103473No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG10800.14363860526914282No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA10670.14190962205756982No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10310.1371216685485984No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC10300.13698866984001584No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG10190.1355256840456079No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10190.1355256840456079No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9920.13193471891387934No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9810.1304717331194714No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC9800.1303387344108889No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT9420.12528478348475236No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG9400.1250187860675873No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT9380.1247527886504222No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9070.12062982868436348No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC9040.12023083255861586No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA9030.12009783385003331No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC8940.11890084547279045No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC8860.11783685580413014No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT8760.11650686871830475No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG8620.11464488679814919No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA8570.1139798932552365No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA8500.11304890229515872No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8460.11251690746082856No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG8400.11171891520933332No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT8250.10972393458059523No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT8230.10945793716343015No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC8170.10865994491193491No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA7880.10480298236304125No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC7870.10466998365445872No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT7850.10440398623729363No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC7780.10347299527721586No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.10347299527721586No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7770.10333999656863331No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG7710.10254200431713807No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG7700.10240900560855554No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7670.10201000948280792No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7670.10201000948280792No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA7640.10161101335706031No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7590.10094601981414761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGT401.5990936E-632.3756
AGCAACG352.3872171E-531.7142872
CTTGCCC303.5983048E-430.83333426
CTAGAGT250.0054951529.5999989
TAGAGTC250.0054951529.59999810
AGTCCAT250.0054951529.59999813
CAGCCTA250.0054951529.59999831
GAGTCCA250.0054951529.59999812
ATTCACG250.0054951529.59999832
AATTCAC250.0054951529.59999831
ATTCTAG250.0054951529.5999986
TGTCAAC405.9374397E-527.752
CTAGTGG1100.026.909099
GAGCTAT358.866529E-426.42857213
TAACAAC451.3226175E-424.6666661
ACTAGTG2050.023.4634158
GGTCCGT400.001930569523.12519
GGGAGCT400.001930569523.12511
TAGCAAC400.001930569523.1251
TTCCCTG400.001930569523.12536
Produced by FastQC (version 0.11.5)