FastQCFastQC Report
Fri 10 Feb 2017
ERR1633485.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633485.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580899
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28050.4828722376867579No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19930.3430889018572936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10760.18523013467057098No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10520.18109860750319764No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9520.16388391097247543No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9010.15510441574180708No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8980.15458797484588543No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8350.14374271603153044No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8140.14012762976007875No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC7980.13737327831516322No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7340.126355872535501No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7220.12429010895181433No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7200.1239458150211999No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6730.11585490765176046No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6480.1115512335190799No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT6380.10982976386600769No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6280.10810829421293547No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6190.10655897152517047No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6180.10638682455986326No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6080.10466535490679103No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6080.10466535490679103No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6070.10449320794148381No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5980.10294388525371881No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG5930.1020831504271827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG451.3221118E-424.6666665
GGTATCA5800.023.6034491
GTATCAA16000.023.5875021
CGAGTTC802.7199349E-823.12500214
GGTCGGC400.001930043223.12500222
CCGAGTT705.0947583E-621.14285713
ACTGTAT759.252837E-619.7333348
TAATACT2750.018.8363654
AACGTCA500.00703079518.528
GCGACCC609.2293177E-418.521
ATTAGAG2500.018.53
CGACCCA500.00703079518.522
TTCGTGT500.00703079518.514
AAGGTCG500.00703079518.520
ATAGACC500.00703079518.53
ATAGAAC609.2293177E-418.53
TCGTGTT500.00703079518.515
TATTAGA2950.017.559322
GGCACCG2650.017.452839
GTATTCC852.7193613E-517.4117641
Produced by FastQC (version 0.11.5)