##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633485.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	580899
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41670927304058	33.0	33.0	33.0	27.0	33.0
2	31.61745329222464	33.0	33.0	33.0	27.0	33.0
3	31.356717777100666	33.0	33.0	33.0	27.0	33.0
4	31.667759799896366	33.0	33.0	33.0	27.0	33.0
5	31.77202233090434	33.0	33.0	33.0	27.0	33.0
6	34.75135953065851	37.0	37.0	37.0	27.0	37.0
7	34.92548102165781	37.0	37.0	37.0	27.0	37.0
8	35.065947780939545	37.0	37.0	37.0	33.0	37.0
9	35.205364443732904	37.0	37.0	37.0	33.0	37.0
10	35.198664483843146	37.0	37.0	37.0	33.0	37.0
11	35.20555380539474	37.0	37.0	37.0	33.0	37.0
12	35.20467929881098	37.0	37.0	37.0	33.0	37.0
13	35.209993475630014	37.0	37.0	37.0	33.0	37.0
14	35.20998831122105	37.0	37.0	37.0	33.0	37.0
15	35.20677432737877	37.0	37.0	37.0	33.0	37.0
16	35.18526972847259	37.0	37.0	37.0	33.0	37.0
17	35.187832996786014	37.0	37.0	37.0	33.0	37.0
18	35.16332615480488	37.0	37.0	37.0	33.0	37.0
19	35.144384824212125	37.0	37.0	37.0	33.0	37.0
20	35.14848536492575	37.0	37.0	37.0	33.0	37.0
21	35.14584635194758	37.0	37.0	37.0	33.0	37.0
22	35.04643492242197	37.0	37.0	37.0	27.0	37.0
23	35.08770371441507	37.0	37.0	37.0	33.0	37.0
24	35.08318657804541	37.0	37.0	37.0	33.0	37.0
25	35.09338284280056	37.0	37.0	37.0	33.0	37.0
26	34.98159748940866	37.0	37.0	37.0	27.0	37.0
27	34.99072127856994	37.0	37.0	37.0	27.0	37.0
28	35.00832502724226	37.0	37.0	37.0	27.0	37.0
29	35.009208141174284	37.0	37.0	37.0	27.0	37.0
30	34.99470131640784	37.0	37.0	37.0	27.0	37.0
31	34.98883110489087	37.0	37.0	37.0	27.0	37.0
32	34.98063518787259	37.0	37.0	37.0	27.0	37.0
33	34.9768858269682	37.0	37.0	37.0	27.0	37.0
34	34.94728687775328	37.0	37.0	37.0	27.0	37.0
35	34.88602665867905	37.0	37.0	37.0	27.0	37.0
36	34.91175057970491	37.0	37.0	37.0	27.0	37.0
37	34.91274731063403	37.0	37.0	37.0	27.0	37.0
38	34.895165941067205	37.0	37.0	37.0	27.0	37.0
39	34.81453918839592	37.0	37.0	37.0	27.0	37.0
40	34.5825246729638	37.0	37.0	37.0	27.0	37.0
41	34.7675189662919	37.0	37.0	37.0	27.0	37.0
42	34.79461834157057	37.0	37.0	37.0	27.0	37.0
43	34.406731634931376	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	21.0
16	37.0
17	31.0
18	20.0
19	31.0
20	70.0
21	222.0
22	530.0
23	1155.0
24	2194.0
25	3742.0
26	5693.0
27	8272.0
28	11114.0
29	14512.0
30	18209.0
31	23215.0
32	29445.0
33	37754.0
34	53440.0
35	102499.0
36	268690.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.28308019122085	20.834602917202474	13.42195459107349	24.460362300503185
2	16.723561238700704	21.56674396065409	38.37207500787572	23.337619792769484
3	17.896742807269423	27.56640999554139	29.616852499315716	24.919994697873467
4	12.605633681586644	18.083866558558373	39.626854238000064	29.683645521854917
5	13.904998975725558	37.49016610460683	35.15206602180413	13.452768897863484
6	29.35450052418751	40.14639377929726	17.23191122725293	13.267194469262297
7	26.887118070439097	32.14810147719311	23.352940872681827	17.61183957968597
8	24.615466716244995	35.6707448282748	20.29440574006841	19.4193827154118
9	25.97215694983121	15.212799471164523	20.332450219401306	38.482593359602966
10	15.520426098168528	27.663845177905284	33.493085717138435	23.322643006787754
11	34.00763299644172	23.93032179432225	23.84734695704417	18.21469825219186
12	23.441424412849738	26.036195620925497	29.43110592374922	21.09127404247554
13	27.741655606224146	22.522503912039788	25.962688866739313	23.773151614996753
14	22.041180997040794	20.92911160115614	27.862847069800427	29.166860332002635
15	24.479814907582902	28.33022608060954	24.89382835914677	22.29613065266079
16	22.98712857140398	28.081301568775295	25.14051496043202	23.791054899388705
17	22.430405285600422	27.580698193661895	26.041704323815328	23.947192196922355
18	22.659705043389643	27.151191515220376	27.97216039277052	22.216943048619466
19	25.05306430205595	25.997634700696683	27.39426303023417	21.5550379670132
20	24.26618052363664	26.02724397872952	27.797947663879608	21.90862783375423
21	23.303878987569266	26.938073572170033	27.591715599441557	22.166331840819144
22	22.84252512054591	26.612199366843463	27.457957407397842	23.08731810521278
23	22.757484519684144	26.696207085913386	28.15033250186349	22.395975892538978
24	23.051339389463575	27.44160344569366	26.197841621348978	23.309215543493792
25	23.20747668699722	26.785207066977218	27.788135286857095	22.21918095916846
26	23.3142078054877	27.057027125197326	27.68002699264416	21.948738076670814
27	23.292517287858992	26.942205099337407	26.650760287072277	23.114517325731324
28	22.28235889543621	26.667802836637694	28.360007505607687	22.689830762318405
29	24.372223054265888	26.491180050232483	26.78382989125476	22.352767004246864
30	23.578625544199593	26.080437391009454	27.87283159378825	22.468105471002705
31	23.22503567745856	26.362241973217376	27.633891605941823	22.77883074338224
32	21.86163171222536	26.369299998794972	27.997465996670677	23.77160229230899
33	22.21246722752148	26.50495180745706	28.37050847049143	22.91207249453003
34	22.984890660854983	26.479129762660982	27.961659427886776	22.57432014859726
35	23.305256163291727	27.116589975193623	27.23433849946376	22.34381536205089
36	24.09386141136411	26.671417922909146	26.632168414819102	22.602552250907646
37	23.025689491632797	26.289079512961806	27.190268876345115	23.494962119060283
38	23.524227103162513	26.655924696031498	26.868009757289997	22.951838443516
39	22.48583660842935	26.80052814688956	27.56761502429854	23.146020220382546
40	22.88538971490741	27.382384889627975	27.644392570825566	22.08783282463905
41	22.667795950759082	25.94495772931267	27.761452507234473	23.625793812693775
42	22.189399534170313	27.496862621557277	27.527160487451347	22.786577356821066
43	23.55676287960558	25.655062239735305	27.375326864050376	23.412848016608738
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	30.0
2	38.0
3	150.0
4	262.0
5	262.0
6	333.5
7	405.0
8	377.5
9	350.0
10	502.0
11	654.0
12	654.0
13	1045.0
14	1436.0
15	2531.0
16	3626.0
17	4111.0
18	4596.0
19	4596.0
20	4178.5
21	3761.0
22	3981.0
23	4201.0
24	5308.0
25	6415.0
26	6415.0
27	8415.5
28	10416.0
29	12638.5
30	14861.0
31	17714.5
32	20568.0
33	20568.0
34	25932.5
35	31297.0
36	33963.5
37	36630.0
38	39344.5
39	42059.0
40	42059.0
41	44259.5
42	46460.0
43	47720.5
44	48981.0
45	47477.0
46	45973.0
47	45973.0
48	44417.0
49	42861.0
50	42680.0
51	42499.0
52	40857.5
53	39216.0
54	39216.0
55	38570.0
56	37924.0
57	32398.0
58	26872.0
59	23990.5
60	21109.0
61	21109.0
62	18827.0
63	16545.0
64	13197.0
65	9849.0
66	8159.0
67	6469.0
68	6469.0
69	5475.5
70	4482.0
71	3823.5
72	3165.0
73	3068.0
74	2971.0
75	2971.0
76	2541.0
77	2111.0
78	1572.0
79	1033.0
80	673.0
81	313.0
82	313.0
83	256.0
84	199.0
85	175.0
86	151.0
87	111.5
88	72.0
89	72.0
90	51.5
91	31.0
92	22.5
93	14.0
94	7.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	580899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.096753306065786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.74251066474869	42.9542140461463
2	7.581122340969684	7.595792313973244
3	2.0779687611928956	3.1229846522157487
4	1.0184350874190375	2.040811653306923
5	0.6185941769674886	1.5494779940054542
6	0.4270055505063475	1.2834955034422382
7	0.3096922881402229	1.0860204711826253
8	0.23348818298418297	0.935759992227213
9	0.19384241996986148	0.8739788304132856
>10	1.4573206759106907	14.739489322341976
>50	0.18979480892543496	6.625323858375226
>100	0.13971979455462216	12.925407401453052
>500	0.009104548016090667	3.052241631078741
>1k	0.0014006996947831796	1.215002329838
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2805	0.4828722376867579	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1993	0.3430889018572936	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1076	0.18523013467057098	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1052	0.18109860750319764	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	952	0.16388391097247543	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	901	0.15510441574180708	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	898	0.15458797484588543	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	835	0.14374271603153044	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	814	0.14012762976007875	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	798	0.13737327831516322	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	734	0.126355872535501	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	722	0.12429010895181433	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	720	0.1239458150211999	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	673	0.11585490765176046	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	648	0.1115512335190799	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	638	0.10982976386600769	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	628	0.10810829421293547	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	619	0.10655897152517047	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	618	0.10638682455986326	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	608	0.10466535490679103	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	608	0.10466535490679103	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	607	0.10449320794148381	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	598	0.10294388525371881	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	593	0.1020831504271827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.7214696530722207E-4	0.0
4	0.0	0.0	0.0	1.7214696530722207E-4	0.0
5	1.7214696530722207E-4	0.0	0.0	1.7214696530722207E-4	0.0
6	1.7214696530722207E-4	0.0	1.7214696530722207E-4	1.7214696530722207E-4	0.0
7	1.7214696530722207E-4	0.0	5.164408959216662E-4	1.7214696530722207E-4	0.0
8	1.7214696530722207E-4	0.0	6.885878612288883E-4	1.7214696530722207E-4	0.0
9	1.7214696530722207E-4	0.0	0.0013771757224577766	1.7214696530722207E-4	0.0
10	1.7214696530722207E-4	0.0	0.0013771757224577766	5.164408959216662E-4	1.7214696530722207E-4
11	3.4429393061444414E-4	0.0	0.002754351444915553	5.164408959216662E-4	1.7214696530722207E-4
12	3.4429393061444414E-4	0.0	0.0029264984102227755	5.164408959216662E-4	1.7214696530722207E-4
13	3.4429393061444414E-4	0.0	0.0030986453755299974	5.164408959216662E-4	1.7214696530722207E-4
14	3.4429393061444414E-4	0.0	0.0034429393061444417	5.164408959216662E-4	1.7214696530722207E-4
15	3.4429393061444414E-4	0.0	0.0037872332367588856	5.164408959216662E-4	1.7214696530722207E-4
16	3.4429393061444414E-4	0.0	0.00413152716737333	5.164408959216662E-4	1.7214696530722207E-4
17	3.4429393061444414E-4	0.0	0.00413152716737333	5.164408959216662E-4	1.7214696530722207E-4
18	3.4429393061444414E-4	0.0	0.0048201150286022185	5.164408959216662E-4	1.7214696530722207E-4
19	3.4429393061444414E-4	0.0	0.0049922619939094404	5.164408959216662E-4	1.7214696530722207E-4
20	3.4429393061444414E-4	0.0	0.0049922619939094404	0.0012050287571505546	1.7214696530722207E-4
21	3.4429393061444414E-4	0.0	0.0049922619939094404	0.0015493226877649987	3.4429393061444414E-4
22	3.4429393061444414E-4	0.0	0.005164408959216662	0.002065763583686665	3.4429393061444414E-4
23	3.4429393061444414E-4	0.0	0.005164408959216662	0.002237910548993887	3.4429393061444414E-4
24	3.4429393061444414E-4	0.0	0.005508702889831106	0.002582204479608331	3.4429393061444414E-4
25	3.4429393061444414E-4	0.0	0.005508702889831106	0.0029264984102227755	3.4429393061444414E-4
26	3.4429393061444414E-4	0.0	0.005508702889831106	0.0034429393061444417	3.4429393061444414E-4
27	3.4429393061444414E-4	0.0	0.005508702889831106	0.004475821097987774	3.4429393061444414E-4
28	3.4429393061444414E-4	0.0	0.005508702889831106	0.014460345085806655	3.4429393061444414E-4
29	5.164408959216662E-4	0.0	0.005508702889831106	0.03047001285937831	3.4429393061444414E-4
30	5.164408959216662E-4	0.0	0.005508702889831106	0.05784138034322662	3.4429393061444414E-4
31	6.885878612288883E-4	0.0	0.005508702889831106	0.1051817958027127	3.4429393061444414E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	45	1.3221118E-4	24.666666	5
GGTATCA	580	0.0	23.603449	1
GTATCAA	1600	0.0	23.587502	1
CGAGTTC	80	2.7199349E-8	23.125002	14
GGTCGGC	40	0.0019300432	23.125002	22
CCGAGTT	70	5.0947583E-6	21.142857	13
ACTGTAT	75	9.252837E-6	19.733334	8
TAATACT	275	0.0	18.836365	4
AACGTCA	50	0.007030795	18.5	28
GCGACCC	60	9.2293177E-4	18.5	21
ATTAGAG	250	0.0	18.5	3
CGACCCA	50	0.007030795	18.5	22
TTCGTGT	50	0.007030795	18.5	14
AAGGTCG	50	0.007030795	18.5	20
ATAGACC	50	0.007030795	18.5	3
ATAGAAC	60	9.2293177E-4	18.5	3
TCGTGTT	50	0.007030795	18.5	15
TATTAGA	295	0.0	17.55932	2
GGCACCG	265	0.0	17.45283	9
GTATTCC	85	2.7193613E-5	17.411764	1
>>END_MODULE