FastQCFastQC Report
Fri 10 Feb 2017
ERR1633484.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633484.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870444
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76290.8764492603774624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57890.6650628874459471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29380.33752889330043057No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16300.1872607542817229No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12270.1409625432537877No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11440.13142717969220308No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10910.12533833308058875No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10220.11741134409565693No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8980.10316574070244611No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8830.10144248222746091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT551.9019702E-523.54545628
ACGTATA551.9019702E-523.54545629
GCCGCTC2150.023.23255727
CGTCAGT400.001930812723.12515
AGCTTCG2300.022.5217421
GCCGGCA2300.022.5217415
GCTTCGC2350.022.04255122
CGCCGCT2450.021.89795926
ACGGTGT603.7249476E-521.58333435
CCGCTCT2450.021.14285728
TTCGCCG2450.021.14285724
GGACGTA806.9561247E-720.812527
GTATCAA34750.020.6561151
ACAACGC450.003825073820.5555553
TGCATCA2950.019.44067814
CGCTCTC2500.019.2429
CCGGCAG2600.019.21153816
TGCTCGC2700.019.18518410
CTTCGCC2900.018.50000223
TCGCCGC2800.018.525
Produced by FastQC (version 0.11.5)