##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633484.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	870444
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.858295306762987	33.0	33.0	33.0	33.0	33.0
2	31.970184181865807	33.0	33.0	33.0	33.0	33.0
3	31.689103492010975	33.0	33.0	33.0	27.0	33.0
4	31.969137589552	33.0	33.0	33.0	33.0	33.0
5	32.07475265496689	33.0	33.0	33.0	33.0	33.0
6	35.23463887395398	37.0	37.0	37.0	33.0	37.0
7	35.388963563422806	37.0	37.0	37.0	33.0	37.0
8	35.52381083676836	37.0	37.0	37.0	33.0	37.0
9	35.64644939823814	37.0	37.0	37.0	33.0	37.0
10	35.64781881430626	37.0	37.0	37.0	33.0	37.0
11	35.66993166705727	37.0	37.0	37.0	33.0	37.0
12	35.65674873972363	37.0	37.0	37.0	33.0	37.0
13	35.658859156936	37.0	37.0	37.0	33.0	37.0
14	35.639009516982135	37.0	37.0	37.0	33.0	37.0
15	35.649998161857624	37.0	37.0	37.0	33.0	37.0
16	35.63866601412612	37.0	37.0	37.0	33.0	37.0
17	35.6492077606371	37.0	37.0	37.0	33.0	37.0
18	35.631392714522704	37.0	37.0	37.0	33.0	37.0
19	35.61570072284949	37.0	37.0	37.0	33.0	37.0
20	35.62384024704634	37.0	37.0	37.0	33.0	37.0
21	35.62479493224148	37.0	37.0	37.0	33.0	37.0
22	35.52924829167643	37.0	37.0	37.0	33.0	37.0
23	35.584793507681134	37.0	37.0	37.0	33.0	37.0
24	35.57244808396634	37.0	37.0	37.0	33.0	37.0
25	35.59006323209764	37.0	37.0	37.0	33.0	37.0
26	35.47960236385109	37.0	37.0	37.0	33.0	37.0
27	35.49681886485518	37.0	37.0	37.0	33.0	37.0
28	35.50697575030674	37.0	37.0	37.0	33.0	37.0
29	35.516273304198776	37.0	37.0	37.0	33.0	37.0
30	35.50913671643437	37.0	37.0	37.0	33.0	37.0
31	35.48908488081944	37.0	37.0	37.0	33.0	37.0
32	35.480356002224156	37.0	37.0	37.0	33.0	37.0
33	35.47174545404414	37.0	37.0	37.0	33.0	37.0
34	35.4288236807882	37.0	37.0	37.0	33.0	37.0
35	35.360112770034604	37.0	37.0	37.0	33.0	37.0
36	35.37460307613126	37.0	37.0	37.0	33.0	37.0
37	35.35587355418614	37.0	37.0	37.0	33.0	37.0
38	35.332147731502545	37.0	37.0	37.0	33.0	37.0
39	35.22247381795957	37.0	37.0	37.0	33.0	37.0
40	34.987407575903795	37.0	37.0	37.0	27.0	37.0
41	35.13916001488895	37.0	37.0	37.0	33.0	37.0
42	35.15412938684166	37.0	37.0	37.0	33.0	37.0
43	34.80696747866606	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	21.0
16	41.0
17	37.0
18	21.0
19	23.0
20	54.0
21	142.0
22	394.0
23	903.0
24	1911.0
25	3345.0
26	5536.0
27	8384.0
28	11933.0
29	16377.0
30	21404.0
31	27554.0
32	35282.0
33	46638.0
34	66877.0
35	135955.0
36	487607.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73950305820938	20.378565421784746	13.937255010086808	23.944676509919073
2	15.848693310540368	21.8953775314667	39.035136091465965	23.22079306652697
3	17.61756069316349	28.07716521683187	30.12566000799592	24.179614082008722
4	12.02753996810823	17.861574093221392	39.96064077643134	30.15024516223904
5	13.413384433691311	37.49994255805083	35.05153691679189	14.035136091465963
6	28.687658252569953	40.453952235870425	17.13906925660927	13.719320254950349
7	26.05612767736925	32.6935449035205	23.651607685273262	17.598719733836983
8	24.863058393187845	35.04763086424859	20.596040641327875	19.49327010123569
9	26.576321969018114	14.848284323862304	20.391202650601304	38.18419105651829
10	15.319538074821585	27.875543975258605	33.54173272490821	23.263185225011604
11	33.19891917228449	24.163760104038857	23.988217507387034	18.649103216289618
12	23.252615906365026	26.469709711365695	29.6592313807666	20.61844300150268
13	27.694257183690162	22.591229303665717	26.32840251641691	23.386110996227213
14	22.068048030660215	21.064766946523843	28.766928142419275	28.100256880396675
15	23.9419192963591	28.464094186415206	25.657710317952677	21.936276199273014
16	22.11250809931483	28.66112007205518	25.621407006079654	23.604964822550333
17	21.92869386198308	27.37154831327461	26.791844162289593	23.907913662452724
18	22.80709614863219	26.742444086006685	28.773936060217544	21.676523705143584
19	24.5698746846437	25.88069996461576	28.67904196019503	20.870383390545516
20	23.699169619182854	26.05854023923423	28.81299658565054	21.429293555932375
21	22.559061812132658	26.926717858931763	28.84321105091195	21.67100927802363
22	22.29264605189995	26.79873719618953	28.63033118730211	22.278285564608407
23	22.04196938573877	26.811948844497753	29.233127001851926	21.91295476791155
24	22.39719039938238	27.321114281906706	27.827982041349014	22.4537132773619
25	22.320333071398046	26.88834663688876	29.0774593196116	21.713860972101596
26	22.496679855338193	27.13615120559163	28.89628741194149	21.470881527128682
27	22.536429684161185	26.8757094080722	28.22065520584897	22.36720570191764
28	21.703751189048347	26.684772369043845	29.39350492392388	22.217971517983926
29	23.344523025030902	26.58367453851138	28.416302484708954	21.655499951748762
30	22.720588573187936	26.43535942576432	28.936611660256144	21.907440340791595
31	22.153176999324483	26.862497759763983	28.939713525511117	22.044611715400418
32	21.31050360505673	26.636865783439255	29.061490457743407	22.991140153760607
33	21.411716319487525	26.76438691058816	29.54090096548428	22.282995804440034
34	22.38087688581919	26.674777469888934	29.017489924682117	21.92685571960976
35	22.234170147648786	27.282972827660366	28.525557072023012	21.95729995266783
36	22.892914420686456	27.232998331885796	27.94527850154634	21.928808745881412
37	22.13985046711793	26.67627096056725	28.480292816080073	22.703585756234748
38	22.43280440786541	26.964055125889775	28.178837466855995	22.424302999388818
39	21.655499951748762	27.168433581023017	28.492470509303296	22.683595957924922
40	22.034272164550504	27.563174655692958	28.70661409579479	21.69593908396175
41	21.76107825431619	26.632385311404295	28.749350905974424	22.857185528305095
42	21.520511371208258	27.686330194705235	28.400678274535757	22.392480159550757
43	22.312406082413112	26.438346407120967	28.409064799114013	22.840182711351908
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	93.5
2	144.0
3	495.0
4	846.0
5	846.0
6	1105.0
7	1364.0
8	1324.5
9	1285.0
10	1760.0
11	2235.0
12	2235.0
13	3493.0
14	4751.0
15	7685.0
16	10619.0
17	11557.5
18	12496.0
19	12496.0
20	10795.0
21	9094.0
22	8779.5
23	8465.0
24	10489.5
25	12514.0
26	12514.0
27	15525.0
28	18536.0
29	21473.0
30	24410.0
31	28512.5
32	32615.0
33	32615.0
34	39623.0
35	46631.0
36	50318.0
37	54005.0
38	57457.5
39	60910.0
40	60910.0
41	62983.0
42	65056.0
43	66658.5
44	68261.0
45	67194.0
46	66127.0
47	66127.0
48	63182.5
49	60238.0
50	61380.5
51	62523.0
52	59370.5
53	56218.0
54	56218.0
55	53595.0
56	50972.0
57	44042.5
58	37113.0
59	34032.5
60	30952.0
61	30952.0
62	27483.0
63	24014.0
64	19402.5
65	14791.0
66	12634.0
67	10477.0
68	10477.0
69	8777.5
70	7078.0
71	6108.5
72	5139.0
73	4979.5
74	4820.0
75	4820.0
76	3942.5
77	3065.0
78	2229.5
79	1394.0
80	952.5
81	511.0
82	511.0
83	404.5
84	298.0
85	264.0
86	230.0
87	175.0
88	120.0
89	120.0
90	94.0
91	68.0
92	40.5
93	13.0
94	8.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	870444.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.35466772578046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.96760308269283	45.86772897658856
2	8.010155738961764	8.547583957681168
3	2.0270743897118115	3.2446164155553876
4	0.9572834299737822	2.043021573025863
5	0.5552749077161987	1.4813254098830597
6	0.3680900554928123	1.1783593562389856
7	0.27812728348843724	1.0387572157199663
8	0.20435058448052745	0.8722446027622057
9	0.1664848793571327	0.7994470877519816
>10	1.1907564718169616	12.63228174256785
>50	0.15409762688825426	5.794165422060364
>100	0.11044300905448474	10.915260801700859
>500	0.00851240583479666	2.968451576447929
>1k	0.0013096008976610245	1.0541554990194844
>5k	4.3653363255367477E-4	1.5626003629964031
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7629	0.8764492603774624	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5789	0.6650628874459471	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2938	0.33752889330043057	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1630	0.1872607542817229	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1227	0.1409625432537877	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1144	0.13142717969220308	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1091	0.12533833308058875	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1022	0.11741134409565693	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	898	0.10316574070244611	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	883	0.10144248222746091	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.2976779666469068E-4	0.0	1.1488389833234534E-4	0.0	1.1488389833234534E-4
3	2.2976779666469068E-4	0.0	8.041872883264174E-4	0.0	1.1488389833234534E-4
4	2.2976779666469068E-4	0.0	0.0014934906783204893	0.0	1.1488389833234534E-4
5	4.5953559332938135E-4	0.0	0.002067910169982216	0.0	1.1488389833234534E-4
6	4.5953559332938135E-4	0.0	0.004825123729958504	1.1488389833234534E-4	1.1488389833234534E-4
7	5.744194916617267E-4	0.0	0.010109783053246389	1.1488389833234534E-4	1.1488389833234534E-4
8	5.744194916617267E-4	0.0	0.012752112714890332	2.2976779666469068E-4	1.1488389833234534E-4
9	5.744194916617267E-4	0.0	0.015624210173198965	0.0012637228816557986	1.1488389833234534E-4
10	0.0016083745766528347	0.0	0.01746235254651649	0.001378606779988144	1.1488389833234534E-4
11	0.00172325847498518	0.0	0.025159573734783627	0.0016083745766528347	1.1488389833234534E-4
12	0.00172325847498518	0.0	0.028261438989756953	0.0016083745766528347	1.1488389833234534E-4
13	0.00172325847498518	0.0	0.030214465261406823	0.0016083745766528347	1.1488389833234534E-4
14	0.00172325847498518	0.0	0.03170795593972731	0.0016083745766528347	1.1488389833234534E-4
15	0.00172325847498518	0.0	0.034809821194700635	0.00172325847498518	1.1488389833234534E-4
16	0.00172325847498518	0.0	0.03664796356801816	0.00172325847498518	1.1488389833234534E-4
17	0.0018381423733175254	0.0	0.037452150856344575	0.0018381423733175254	1.1488389833234534E-4
18	0.0019530262716498706	0.0	0.03802657034800631	0.002182794068314561	1.1488389833234534E-4
19	0.0019530262716498706	0.0	0.038945641534665065	0.002412561864979252	1.1488389833234534E-4
20	0.0019530262716498706	0.0	0.040439132212985555	0.002757213559976288	1.1488389833234534E-4
21	0.0019530262716498706	0.0	0.04135820339964432	0.0033316330516380147	1.1488389833234534E-4
22	0.002067910169982216	0.0	0.041702855094641354	0.004020936441632087	1.1488389833234534E-4
23	0.002182794068314561	0.0	0.04262192628130012	0.00516977542495554	1.1488389833234534E-4
24	0.002182794068314561	0.0	0.04285169407796481	0.007352569493270102	1.1488389833234534E-4
25	0.0022976779666469068	0.0	0.04296657797629715	0.008041872883264174	1.1488389833234534E-4
26	0.0022976779666469068	0.0	0.04296657797629715	0.00884606017159059	1.1488389833234534E-4
27	0.0022976779666469068	0.0	0.04296657797629715	0.011373505934902188	1.1488389833234534E-4
28	0.0026423296616439424	0.0	0.04296657797629715	0.028146555091424608	1.1488389833234534E-4
29	0.0028720974583086333	0.0	0.04296657797629715	0.06307126018445759	1.1488389833234534E-4
30	0.0029869813566409786	0.0	0.04296657797629715	0.11603273731566878	1.1488389833234534E-4
31	0.0029869813566409786	0.0	0.04296657797629715	0.20518264242156875	1.1488389833234534E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAT	55	1.9019702E-5	23.545456	28
ACGTATA	55	1.9019702E-5	23.545456	29
GCCGCTC	215	0.0	23.232557	27
CGTCAGT	40	0.0019308127	23.125	15
AGCTTCG	230	0.0	22.52174	21
GCCGGCA	230	0.0	22.52174	15
GCTTCGC	235	0.0	22.042551	22
CGCCGCT	245	0.0	21.897959	26
ACGGTGT	60	3.7249476E-5	21.583334	35
CCGCTCT	245	0.0	21.142857	28
TTCGCCG	245	0.0	21.142857	24
GGACGTA	80	6.9561247E-7	20.8125	27
GTATCAA	3475	0.0	20.656115	1
ACAACGC	45	0.0038250738	20.555555	3
TGCATCA	295	0.0	19.440678	14
CGCTCTC	250	0.0	19.24	29
CCGGCAG	260	0.0	19.211538	16
TGCTCGC	270	0.0	19.185184	10
CTTCGCC	290	0.0	18.500002	23
TCGCCGC	280	0.0	18.5	25
>>END_MODULE