FastQCFastQC Report
Fri 10 Feb 2017
ERR1633483.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633483.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336570
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50811.5096413821790415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43451.2909647324479305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23920.7106991116261105No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11890.35326975072050393No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.18629111328995454No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4930.14647770151825773No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4840.14380366639926315No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4810.1429123213595983No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4150.1233027304869715No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4060.12062869536797695No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3820.11349793505065811No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.11082389993166354No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3660.10874409483911222No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3430.101910449535015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTGG250.005490890229.61
CCCGGCG250.005490890229.620
CGAGTCG751.3664248E-824.66666621
TACATCC400.001928365823.1257
TTATGCG502.6966038E-422.24
TATGCGG502.6966038E-422.25
AGCTTCG1301.8189894E-1221.34615321
GCTTCGC1301.8189894E-1221.34615322
AGTCGCC1059.749783E-1021.14285723
CCGGCAG1151.3460522E-1020.91304216
GCCGCTC1151.3460522E-1020.91304227
CTCTAAT806.9300586E-720.81251
TGCGGAG450.003820272420.5555557
CAAGACA450.003820272420.5555554
GAGTCGC1001.2816599E-820.3522
GCCGGCA1202.3646862E-1020.04166815
CCGCTCT1202.3646862E-1020.04166828
GTATTAT759.233881E-619.7333341
TCTAATA951.6673403E-719.4736822
GGCGAGT951.6673403E-719.47368219
Produced by FastQC (version 0.11.5)