##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633483.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336570
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.70190450723475	33.0	33.0	33.0	27.0	33.0
2	31.113616781055946	33.0	33.0	33.0	27.0	33.0
3	30.882232522209346	33.0	33.0	33.0	27.0	33.0
4	31.216813738598212	33.0	33.0	33.0	27.0	33.0
5	31.356731140624536	33.0	33.0	33.0	27.0	33.0
6	33.765775321627004	37.0	33.0	37.0	27.0	37.0
7	33.98861455269335	37.0	33.0	37.0	27.0	37.0
8	34.153852096146416	37.0	33.0	37.0	27.0	37.0
9	34.20594230026443	37.0	33.0	37.0	27.0	37.0
10	34.134471283833975	37.0	33.0	37.0	27.0	37.0
11	34.191799625635085	37.0	33.0	37.0	27.0	37.0
12	34.24558635647859	37.0	33.0	37.0	27.0	37.0
13	34.262397123926675	37.0	33.0	37.0	27.0	37.0
14	34.306081944320646	37.0	37.0	37.0	27.0	37.0
15	34.35098790741896	37.0	37.0	37.0	27.0	37.0
16	34.356300323855365	37.0	37.0	37.0	27.0	37.0
17	34.356466708262765	37.0	37.0	37.0	27.0	37.0
18	34.34146834239534	37.0	37.0	37.0	27.0	37.0
19	34.304269542739995	37.0	37.0	37.0	27.0	37.0
20	34.29975933683929	37.0	37.0	37.0	27.0	37.0
21	34.30766853849125	37.0	37.0	37.0	27.0	37.0
22	34.181546186528806	37.0	33.0	37.0	27.0	37.0
23	34.22664527438572	37.0	33.0	37.0	27.0	37.0
24	34.2120628695368	37.0	37.0	37.0	27.0	37.0
25	34.228962771488845	37.0	37.0	37.0	27.0	37.0
26	34.08964257063909	37.0	33.0	37.0	27.0	37.0
27	34.09475592001664	37.0	33.0	37.0	27.0	37.0
28	34.08768458270196	37.0	33.0	37.0	27.0	37.0
29	34.082672252428914	37.0	33.0	37.0	27.0	37.0
30	34.06555842766735	37.0	33.0	37.0	27.0	37.0
31	34.04315001337017	37.0	33.0	37.0	27.0	37.0
32	33.98786582286003	37.0	33.0	37.0	27.0	37.0
33	33.93449802418516	37.0	33.0	37.0	27.0	37.0
34	33.875615770864904	37.0	33.0	37.0	27.0	37.0
35	33.76377870873815	37.0	33.0	37.0	27.0	37.0
36	33.773503283120895	37.0	33.0	37.0	27.0	37.0
37	33.71647799863327	37.0	33.0	37.0	27.0	37.0
38	33.67957037169088	37.0	33.0	37.0	27.0	37.0
39	33.54065424725911	37.0	33.0	37.0	22.0	37.0
40	33.25079181151023	37.0	33.0	37.0	14.0	37.0
41	33.385046201384554	37.0	33.0	37.0	22.0	37.0
42	33.351995127313785	37.0	33.0	37.0	14.0	37.0
43	32.85810084083549	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	17.0
16	31.0
17	33.0
18	33.0
19	48.0
20	150.0
21	391.0
22	858.0
23	1579.0
24	2694.0
25	4043.0
26	5802.0
27	8007.0
28	10370.0
29	12894.0
30	15867.0
31	19321.0
32	23762.0
33	29676.0
34	40790.0
35	66740.0
36	93462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92985114537838	20.51995127313783	14.067504531003951	22.482693050479842
2	16.295867130166087	24.127224648661496	37.10996226639332	22.466945954779092
3	18.895623495855247	29.237602876073325	30.052292242327006	21.814481385744422
4	12.644917847698844	19.16778084796625	38.395578928603264	29.791722375731645
5	14.391657010428737	37.59336839290489	33.6708559883531	14.344118608313277
6	27.796000831922036	40.85777104317081	16.470570757940397	14.875657366966752
7	25.58457378851353	33.41830822711472	23.10009804795436	17.897019936417387
8	25.711739014172387	34.20506878212556	20.155094036901687	19.928098166800368
9	27.177110259381404	14.30787057670024	20.40259084291529	38.11242832100306
10	16.173752859732	28.12876964673025	32.31155480286419	23.38592269067356
11	33.295896841667414	24.25765813946579	23.302730486971505	19.1437145318953
12	23.486347565142466	27.3886561487952	29.1520337522655	19.97296253379683
13	28.925929227203852	22.730784086519893	26.346673797426984	21.996612888849274
14	22.017113824761562	21.96660427251389	30.019312475859405	25.996969426865142
15	24.615384615384617	27.909201651959474	26.929316338354575	20.546097394301334
16	21.03039486585257	28.77529191550049	26.725495439284547	23.468817779362393
17	21.468045280328017	27.204147725584576	27.489081023264106	23.838725970823305
18	22.967584752057522	25.416406691030097	30.041596101851027	21.574412455061353
19	24.44543482782185	25.282704935080368	30.68485010547583	19.587010131621952
20	23.634905071753277	25.00579374275782	30.711887571678997	20.647413613809906
21	21.787443919541253	26.83780491428232	30.676828000118846	20.69792316605758
22	21.68553347000624	26.655673411177467	30.533618563746025	21.125174555070267
23	21.346228124907153	26.616157114418993	31.24550613542502	20.792108625248833
24	21.699200760614435	26.966158599994056	29.98128175416704	21.353358885224473
25	21.52598270790623	26.658644561309686	31.378019431321864	20.43735329946222
26	21.535490388329322	27.213655406007664	30.845292212615504	20.40556199304751
27	21.900644739578688	26.475918828178386	30.656029949193332	20.96740648304959
28	20.84766913272128	26.622099414683426	31.28739935228927	21.24283210030603
29	22.305018272573314	26.769468461241345	30.078735478503727	20.84677778768161
30	21.937487001218173	26.29972962533797	31.017916035297265	20.744867338146598
31	21.356924265383128	26.958433609650296	30.836972992245297	20.84766913272128
32	20.181240158065187	26.907032712362955	30.79478266036783	22.11694446920403
33	20.35980628101138	27.16789969397154	31.347119469946815	21.125174555070267
34	21.11626110467362	27.109665151380096	30.58888195620525	21.185191787741033
35	20.891642154678074	27.84621326915649	30.37139376652702	20.890750809638412
36	21.769617018747958	27.62931930950471	29.695457111447844	20.905606560299493
37	21.2781887868794	27.45105030157174	29.984847134325697	21.285913777223165
38	21.433282823781084	27.584752057521467	29.562052470511336	21.419912648186113
39	21.040496776302106	27.446593576373413	29.695457111447844	21.81745253587664
40	21.030989095879015	28.23008586623882	30.03565380158659	20.70327123629557
41	21.034554476037673	27.224351546483643	29.617612977983775	22.123480999494905
42	20.379118756870785	28.359033781977004	29.607213952521022	21.65463350863119
43	21.243426330332472	27.19285735508215	29.604242802388804	21.95947351219657
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	47.5
2	77.0
3	255.0
4	433.0
5	433.0
6	700.0
7	967.0
8	985.0
9	1003.0
10	1367.0
11	1731.0
12	1731.0
13	2768.0
14	3805.0
15	6075.5
16	8346.0
17	8521.0
18	8696.0
19	8696.0
20	7397.5
21	6099.0
22	5084.5
23	4070.0
24	4485.5
25	4901.0
26	4901.0
27	5652.0
28	6403.0
29	7232.5
30	8062.0
31	9453.5
32	10845.0
33	10845.0
34	13200.5
35	15556.0
36	16857.0
37	18158.0
38	19274.0
39	20390.0
40	20390.0
41	21460.5
42	22531.0
43	23306.5
44	24082.0
45	24897.5
46	25713.0
47	25713.0
48	25366.0
49	25019.0
50	26015.0
51	27011.0
52	25552.5
53	24094.0
54	24094.0
55	22008.5
56	19923.0
57	17085.5
58	14248.0
59	12708.0
60	11168.0
61	11168.0
62	9616.5
63	8065.0
64	6393.0
65	4721.0
66	4119.5
67	3518.0
68	3518.0
69	2908.5
70	2299.0
71	1949.0
72	1599.0
73	1524.0
74	1449.0
75	1449.0
76	1149.0
77	849.0
78	600.0
79	351.0
80	259.5
81	168.0
82	168.0
83	127.0
84	86.0
85	70.5
86	55.0
87	47.0
88	39.0
89	39.0
90	29.5
91	20.0
92	11.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	336570.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.63476421436412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.38390237538269	46.86815000034664
2	6.292211334332057	6.749625426076985
3	1.885806066884841	3.0343429125415744
4	0.9557124543772327	2.050376485890164
5	0.5885666915157935	1.578381786193898
6	0.42690723414368115	1.3738241306821604
7	0.30255524717885945	1.1359235540979749
8	0.2559504080902863	1.0982271830794221
9	0.20209052022124446	0.9755169661822167
>10	1.4381528359754032	15.150238761800495
>50	0.16752025137437432	6.235466672011411
>100	0.09781383805080915	9.639179416092297
>500	5.621484945448803E-4	0.18904490127900753
>1k	0.001686445483634641	2.389744637220755
>5k	5.621484945448803E-4	1.5319571665050036
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5081	1.5096413821790415	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4345	1.2909647324479305	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2392	0.7106991116261105	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1189	0.35326975072050393	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	627	0.18629111328995454	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	493	0.14647770151825773	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	484	0.14380366639926315	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	481	0.1429123213595983	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	415	0.1233027304869715	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	406	0.12062869536797695	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	382	0.11349793505065811	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	373	0.11082389993166354	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	366	0.10874409483911222	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	343	0.101910449535015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0011884600528864725	0.0	0.0
2	0.0	0.0	0.0014855750661080904	0.0	0.0
3	0.0	0.0	0.0020798050925513265	0.0	0.0
4	0.0	0.0	0.002971150132216181	0.0	0.0
5	0.0	0.0	0.0032682651454377988	0.0	0.0
6	2.971150132216181E-4	0.0	0.0062394152776539796	0.0	0.0
7	2.971150132216181E-4	0.0	0.02020382089907003	2.971150132216181E-4	0.0
8	2.971150132216181E-4	0.0	0.022877856018064593	2.971150132216181E-4	0.0
9	5.942300264432362E-4	0.0	0.030008616335383428	0.002674035118994563	0.0
10	0.0014855750661080904	0.0	0.03416822652048608	0.002971150132216181	0.0
11	0.0014855750661080904	0.0	0.04813263214190213	0.0032682651454377988	0.0
12	0.0014855750661080904	0.0	0.05318358736666964	0.0032682651454377988	0.0
13	0.0014855750661080904	0.0	0.056748967525329055	0.0032682651454377988	0.0
14	0.0014855750661080904	0.0	0.059125887631102	0.0032682651454377988	0.0
15	0.0014855750661080904	0.0	0.06358261282942627	0.003862495171881035	0.0
16	0.0014855750661080904	0.0	0.06803933802775054	0.003862495171881035	0.0
17	0.0014855750661080904	0.0	0.0704162581335235	0.004159610185102653	0.0
18	0.0014855750661080904	0.0	0.07368452327896129	0.005348070237989126	0.0
19	0.0014855750661080904	0.0	0.07517009834506938	0.005645185251210744	0.0
20	0.0014855750661080904	0.0	0.07635855839795584	0.0065365302908755975	0.0
21	0.0014855750661080904	0.0	0.07932970853017203	0.00772499034376207	0.0
22	0.0014855750661080904	0.0	0.08022105356983689	0.009804795436313397	0.0
23	0.0014855750661080904	0.0	0.08200374364916659	0.010399025462756633	0.0
24	0.0017826900793297086	0.0	0.0823008586623882	0.013073060581751195	0.0
25	0.0017826900793297086	0.0	0.08259797367560982	0.016341325727188993	0.0
26	0.0017826900793297086	0.0	0.08259797367560982	0.01871824583296194	0.0
27	0.0017826900793297086	0.0	0.08259797367560982	0.024363431084172684	0.0
28	0.0017826900793297086	0.0	0.08259797367560982	0.04813263214190213	0.0
29	0.0020798050925513265	0.0	0.08259797367560982	0.10696140475978251	0.0
30	0.0020798050925513265	0.0	0.08259797367560982	0.18005169801230056	0.0
31	0.0020798050925513265	0.0	0.08259797367560982	0.28433906765308853	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATTGG	25	0.0054908902	29.6	1
CCCGGCG	25	0.0054908902	29.6	20
CGAGTCG	75	1.3664248E-8	24.666666	21
TACATCC	40	0.0019283658	23.125	7
TTATGCG	50	2.6966038E-4	22.2	4
TATGCGG	50	2.6966038E-4	22.2	5
AGCTTCG	130	1.8189894E-12	21.346153	21
GCTTCGC	130	1.8189894E-12	21.346153	22
AGTCGCC	105	9.749783E-10	21.142857	23
CCGGCAG	115	1.3460522E-10	20.913042	16
GCCGCTC	115	1.3460522E-10	20.913042	27
CTCTAAT	80	6.9300586E-7	20.8125	1
TGCGGAG	45	0.0038202724	20.555555	7
CAAGACA	45	0.0038202724	20.555555	4
GAGTCGC	100	1.2816599E-8	20.35	22
GCCGGCA	120	2.3646862E-10	20.041668	15
CCGCTCT	120	2.3646862E-10	20.041668	28
GTATTAT	75	9.233881E-6	19.733334	1
TCTAATA	95	1.6673403E-7	19.473682	2
GGCGAGT	95	1.6673403E-7	19.473682	19
>>END_MODULE