FastQCFastQC Report
Fri 10 Feb 2017
ERR1633482.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633482.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886230
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72570.8188619207203547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54080.6102253365379191No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28370.3201200591268632No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA27790.3135754826625143No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22400.25275605655416766No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG19480.21980749918192793No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT16970.19148528034483148No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16070.1813299030725658No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC14590.16462994933595115No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT14250.1607934734775397No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.1454475700438938No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12050.1359692179231125No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC11170.12603951570134164No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC11130.12558816560035205No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11130.12558816560035205No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11080.12502397797411507No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10960.12366992767114632No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10540.11893075161075568No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10270.11588413842907597No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10160.11464292565135462No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC10040.11328887534838585No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA9230.10414903580334677No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG9000.10155377272265664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAA200.001841708637.010
GGTATCA12250.025.975511
GTATCAA33150.023.7179491
GACCGTT1351.8189894E-1220.5555537
TTAACGG1403.6379788E-1219.8214335
TTTAACG1500.019.73333434
ATTAGAG1650.019.0606063
TAGGACC2150.018.9302334
GGACCGT2250.018.9111126
AACGATT902.1510841E-618.522
AACGGCC1552.0008883E-1117.90322737
CCGCTCT1258.569259E-917.76000228
GGGCTAC1054.794201E-717.6190471
CTTGCTA953.6045021E-617.52631616
TGCGGTA852.7219758E-517.41176436
CGAGGGT1603.45608E-1117.3437516
TTCGCCG1401.8662831E-917.17857224
CGCTCTC1301.3917088E-817.07692329
TCTCGCG650.001579547917.07692320
GTGACAC1851.8189894E-1217.024
Produced by FastQC (version 0.11.5)