##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633482.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	886230
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.444764903016146	33.0	33.0	33.0	27.0	33.0
2	31.64668991119687	33.0	33.0	33.0	27.0	33.0
3	31.38539656748248	33.0	33.0	33.0	27.0	33.0
4	31.700919625830768	33.0	33.0	33.0	27.0	33.0
5	31.8058833485664	33.0	33.0	33.0	33.0	33.0
6	34.819049231012265	37.0	37.0	37.0	27.0	37.0
7	35.004868939214425	37.0	37.0	37.0	33.0	37.0
8	35.16338083793146	37.0	37.0	37.0	33.0	37.0
9	35.295391715468895	37.0	37.0	37.0	33.0	37.0
10	35.289256739221194	37.0	37.0	37.0	33.0	37.0
11	35.30757478306986	37.0	37.0	37.0	33.0	37.0
12	35.30167112374891	37.0	37.0	37.0	33.0	37.0
13	35.28290737167553	37.0	37.0	37.0	33.0	37.0
14	35.293242160612934	37.0	37.0	37.0	33.0	37.0
15	35.293571646186656	37.0	37.0	37.0	33.0	37.0
16	35.270416257630636	37.0	37.0	37.0	33.0	37.0
17	35.27333649278404	37.0	37.0	37.0	33.0	37.0
18	35.25392279656523	37.0	37.0	37.0	33.0	37.0
19	35.23326901594394	37.0	37.0	37.0	33.0	37.0
20	35.22319939519087	37.0	37.0	37.0	33.0	37.0
21	35.214376629091774	37.0	37.0	37.0	33.0	37.0
22	35.1082337542173	37.0	37.0	37.0	33.0	37.0
23	35.14345937284904	37.0	37.0	37.0	33.0	37.0
24	35.153935208693	37.0	37.0	37.0	33.0	37.0
25	35.16598851313993	37.0	37.0	37.0	33.0	37.0
26	35.04579962312267	37.0	37.0	37.0	33.0	37.0
27	35.055431434277786	37.0	37.0	37.0	27.0	37.0
28	35.053180325649095	37.0	37.0	37.0	27.0	37.0
29	35.056760660325196	37.0	37.0	37.0	27.0	37.0
30	35.06465703034201	37.0	37.0	37.0	27.0	37.0
31	35.05720862530043	37.0	37.0	37.0	27.0	37.0
32	35.03441318844995	37.0	37.0	37.0	27.0	37.0
33	35.01959987813547	37.0	37.0	37.0	27.0	37.0
34	34.97539803437031	37.0	37.0	37.0	27.0	37.0
35	34.907819640499646	37.0	37.0	37.0	27.0	37.0
36	34.934058878620675	37.0	37.0	37.0	27.0	37.0
37	34.932678875686896	37.0	37.0	37.0	27.0	37.0
38	34.905437640341674	37.0	37.0	37.0	27.0	37.0
39	34.78708010335917	37.0	37.0	37.0	27.0	37.0
40	34.55734064520497	37.0	37.0	37.0	27.0	37.0
41	34.71613350935987	37.0	37.0	37.0	27.0	37.0
42	34.73667783758167	37.0	37.0	37.0	27.0	37.0
43	34.341597553682455	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	27.0
16	37.0
17	36.0
18	36.0
19	42.0
20	93.0
21	283.0
22	733.0
23	1683.0
24	3108.0
25	5234.0
26	8320.0
27	12216.0
28	16420.0
29	21958.0
30	28036.0
31	35231.0
32	43370.0
33	56230.0
34	79732.0
35	151899.0
36	421504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8885278088081	19.640950994662784	13.530009139839544	23.940512056689574
2	16.879026889182267	22.30899427913747	37.78691761732282	23.025061214357446
3	19.030048632973383	27.119257980433975	29.70414000880133	24.146553377791317
4	12.801417239317106	18.306647258612323	38.924996896968054	29.966938605102513
5	14.792096859731672	36.45520914435305	33.702312040892316	15.050381955022962
6	28.860340995001298	39.70583257168004	17.289078455931303	14.14474797738736
7	26.768558951965066	32.4097581891834	21.940128409103732	18.88155444974781
8	25.25766448890243	33.872132516389655	20.374959096397095	20.49524389831082
9	26.941990228270313	14.27169019329068	19.470340656488723	39.31597892195028
10	16.066483869875768	26.8126784243368	32.166818997325755	24.954018708461685
11	34.69178429978674	23.480924816356925	23.348904911817474	18.47838597203886
12	23.972445076334587	25.20429233946041	29.638242894056848	21.185019690148156
13	30.05980388838112	22.22233505974747	24.937544429775567	22.780316622095846
14	22.95735869920901	20.998724935964706	28.12734843099421	27.916567933832077
15	25.329316317434525	27.219796215429405	24.79209685973167	22.6587906074044
16	22.386965009083422	27.32439660133374	25.272784717285578	25.01585367229726
17	22.9163986775442	26.32409194001557	25.69152477347867	25.06798460896156
18	24.12082642203491	24.967446373966126	27.79718583212033	23.114541371878634
19	26.069981833158433	25.153741128149576	27.44919490425736	21.327082134434626
20	25.958611195739255	24.17690667208287	27.41884160996581	22.445640522212067
21	24.325739367884182	25.664782279995034	27.536079798697855	22.47339855342293
22	23.379935231260507	25.74816921115286	27.516558906830056	23.355336650756577
23	23.58958735317017	25.421617413086896	28.223824515080736	22.764970718662198
24	23.434322918429753	25.587488575200567	27.1678909538156	23.810297552554076
25	23.81413402841249	26.010516457353056	27.93857125125532	22.236778262979136
26	24.19924850208185	25.77197792898006	27.291448043961502	22.737325524976587
27	24.03428004017016	25.73530573327466	27.614501878744797	22.615912347810387
28	23.15313180551324	26.000135405030296	27.316610812091668	23.530121977364793
29	24.279588820058	25.812148087968133	26.594789163084076	23.313473928889792
30	24.14677905284181	25.315888651930084	27.938796926305816	22.598535368922292
31	24.433160691919706	24.967784886541867	27.08834049851619	23.510713923022237
32	22.37229613080126	25.376595240513183	28.000631890141385	24.250476738544172
33	22.826580007447276	25.576881847827316	28.264671699220294	23.331866445505117
34	22.68609728851427	25.728873994335554	27.652979474854156	23.93204924229602
35	23.269015943942318	26.14874242578112	27.304085846789206	23.278155783487357
36	23.85577107522878	25.489771278336327	27.47345497218555	23.18100267424935
37	24.302156325107475	25.407964072531964	26.862214097920408	23.427665504440156
38	23.716416731548243	25.709804452568747	26.660234927727565	23.913543888155445
39	23.388398045654064	25.250555724811846	27.526827121627566	23.834219107906527
40	23.415704726763934	26.19714972411225	27.451000304661317	22.936145244462498
41	23.793597598817463	25.05252586800266	27.419857147693037	23.73401938548684
42	22.819358405831444	26.228518556131025	27.565643230312673	23.386479807724857
43	23.53147602766776	25.6685059183282	27.003938029631136	23.796080024372905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	48.5
2	77.0
3	333.5
4	590.0
5	590.0
6	808.0
7	1026.0
8	1033.0
9	1040.0
10	1365.0
11	1690.0
12	1690.0
13	2701.0
14	3712.0
15	6673.5
16	9635.0
17	10497.0
18	11359.0
19	11359.0
20	9512.0
21	7665.0
22	6921.5
23	6178.0
24	7238.0
25	8298.0
26	8298.0
27	10392.0
28	12486.0
29	14805.0
30	17124.0
31	20229.0
32	23334.0
33	23334.0
34	28904.0
35	34474.0
36	36789.0
37	39104.0
38	43028.0
39	46952.0
40	46952.0
41	51837.5
42	56723.0
43	61365.0
44	66007.0
45	68711.5
46	71416.0
47	71416.0
48	74668.5
49	77921.0
50	77720.0
51	77519.0
52	77277.5
53	77036.0
54	77036.0
55	71398.0
56	65760.0
57	58611.0
58	51462.0
59	45662.0
60	39862.0
61	39862.0
62	34332.0
63	28802.0
64	23101.5
65	17401.0
66	14861.0
67	12321.0
68	12321.0
69	10183.0
70	8045.0
71	6525.0
72	5005.0
73	3903.0
74	2801.0
75	2801.0
76	2092.5
77	1384.0
78	1066.0
79	748.0
80	624.0
81	500.0
82	500.0
83	414.5
84	329.0
85	277.5
86	226.0
87	173.5
88	121.0
89	121.0
90	93.0
91	65.0
92	37.0
93	9.0
94	6.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	886230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.438364272948405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.29713353507834	38.21744265721446
2	9.426273771723775	8.754814702956958
3	2.8522759561218516	3.973650895720762
4	1.3613698975234803	2.528791648456873
5	0.8477632356135347	1.9684368976317357
6	0.5773089832241071	1.6085570916603924
7	0.3787824244647655	1.231302534523974
8	0.2784957560117435	1.0346309892914805
9	0.2281809530629597	0.9536715196637637
>10	1.4250273833298557	12.69293643562291
>50	0.17520113042771174	5.745796891728832
>100	0.13338012792278056	12.386760777604335
>500	0.013610217134676368	4.2147622695513896
>1k	0.004701711373797291	3.2330376446153943
>5k	4.949169867155043E-4	1.4554070437568054
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7257	0.8188619207203547	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5408	0.6102253365379191	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2837	0.3201200591268632	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	2779	0.3135754826625143	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2240	0.25275605655416766	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1948	0.21980749918192793	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1697	0.19148528034483148	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1607	0.1813299030725658	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1459	0.16462994933595115	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1425	0.1607934734775397	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1289	0.1454475700438938	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1205	0.1359692179231125	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	1117	0.12603951570134164	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	1113	0.12558816560035205	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1113	0.12558816560035205	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1108	0.12502397797411507	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1096	0.12366992767114632	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1054	0.11893075161075568	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1027	0.11588413842907597	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	1016	0.11464292565135462	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	1004	0.11328887534838585	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	923	0.10414903580334677	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	900	0.10155377272265664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.1283752524739628E-4	0.0	1.1283752524739628E-4	0.0	0.0
3	1.1283752524739628E-4	0.0	2.2567505049479256E-4	0.0	0.0
4	1.1283752524739628E-4	0.0	5.641876262369814E-4	0.0	0.0
5	2.2567505049479256E-4	0.0	7.898626767317739E-4	0.0	0.0
6	2.2567505049479256E-4	0.0	0.0022567505049479256	0.0	0.0
7	2.2567505049479256E-4	0.0	0.006770251514843777	0.0	0.0
8	3.385125757421888E-4	0.0	0.0077857892420703425	0.0	0.0
9	3.385125757421888E-4	0.0	0.00880132696929691	2.2567505049479256E-4	0.0
10	6.770251514843776E-4	0.0	0.009816864696523476	3.385125757421888E-4	0.0
11	6.770251514843776E-4	0.0	0.013766178080182346	4.513501009895851E-4	0.0
12	6.770251514843776E-4	0.0	0.01455604075691412	4.513501009895851E-4	0.0
13	6.770251514843776E-4	0.0	0.015684416009388084	4.513501009895851E-4	0.0
14	6.770251514843776E-4	0.0	0.01692562878710944	6.770251514843776E-4	0.0
15	7.898626767317739E-4	0.0	0.01794116651433601	7.898626767317739E-4	0.0
16	7.898626767317739E-4	0.0	0.019182379292057367	7.898626767317739E-4	0.0
17	7.898626767317739E-4	0.0	0.019408054342552158	7.898626767317739E-4	0.0
18	7.898626767317739E-4	0.0	0.01997224196878914	9.027002019791702E-4	0.0
19	7.898626767317739E-4	0.0	0.02053642959502612	9.027002019791702E-4	0.0
20	7.898626767317739E-4	0.0	0.021100617221263104	0.0013540503029687552	0.0
21	7.898626767317739E-4	0.0	0.021439129797005293	0.002143912979700529	0.0
22	7.898626767317739E-4	0.0	0.021551967322252688	0.002482425555442718	0.0
23	7.898626767317739E-4	0.0	0.021551967322252688	0.002820938131184907	0.0
24	7.898626767317739E-4	0.0	0.021777642372747482	0.004174988434153662	0.0
25	7.898626767317739E-4	0.0	0.021890479897994877	0.004626338535143247	0.0
26	7.898626767317739E-4	0.0	0.021890479897994877	0.004964851110885436	0.0
27	7.898626767317739E-4	0.0	0.021890479897994877	0.007108764090585965	0.0
28	7.898626767317739E-4	0.0	0.021890479897994877	0.02516276813016937	0.0
29	7.898626767317739E-4	0.0	0.021890479897994877	0.06544576464348983	0.0
30	7.898626767317739E-4	0.0	0.021890479897994877	0.11069361226769575	0.0
31	7.898626767317739E-4	0.0	0.021890479897994877	0.200173769788881	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCAA	20	0.0018417086	37.0	10
GGTATCA	1225	0.0	25.97551	1
GTATCAA	3315	0.0	23.717949	1
GACCGTT	135	1.8189894E-12	20.555553	7
TTAACGG	140	3.6379788E-12	19.82143	35
TTTAACG	150	0.0	19.733334	34
ATTAGAG	165	0.0	19.060606	3
TAGGACC	215	0.0	18.930233	4
GGACCGT	225	0.0	18.911112	6
AACGATT	90	2.1510841E-6	18.5	22
AACGGCC	155	2.0008883E-11	17.903227	37
CCGCTCT	125	8.569259E-9	17.760002	28
GGGCTAC	105	4.794201E-7	17.619047	1
CTTGCTA	95	3.6045021E-6	17.526316	16
TGCGGTA	85	2.7219758E-5	17.411764	36
CGAGGGT	160	3.45608E-11	17.34375	16
TTCGCCG	140	1.8662831E-9	17.178572	24
CGCTCTC	130	1.3917088E-8	17.076923	29
TCTCGCG	65	0.0015795479	17.076923	20
GTGACAC	185	1.8189894E-12	17.0	24
>>END_MODULE