FastQCFastQC Report
Fri 10 Feb 2017
ERR1633481.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633481.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369691
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28110.7603647370371472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25860.6995030985336403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15060.4073672337168067No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13230.35786643440062105No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8470.22911025694431295No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC8030.21720842541473828No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT7870.2128804866767111No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7670.20747056325417712No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7100.19205228149995537No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7020.1898883121309418No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6980.188806327446435No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6870.18583086956404132No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6690.18096193848376077No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6460.17474052654784672No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6280.16987159546756617No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.16987159546756617No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6000.16229770267601862No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5880.15905174862249824No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5620.1520188481732041No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG5500.14877289411968372No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5500.14877289411968372No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5370.14525644389503667No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5330.14417445921052988No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4960.13416610087884207No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4840.1309201468253217No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4820.13037915448306828No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4740.12821518511405472No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT4700.12713320042954793No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4690.12686270425842122No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4560.12334625403377417No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4550.12307575786264745No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4540.12280526169152076No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4500.12172327700701395No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4470.12091178849363386No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGC4460.12064129232250717No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4450.12037079615138048No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4420.11955930763800039No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG4410.11928881146687367No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4390.11874781912462029No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT4380.11847732295349359No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4350.1176658344401135No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4350.1176658344401135No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4330.11712484209786012No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA4300.11631335358448001No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT4250.11496087272884652No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4240.11469037655771983No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC4200.11360839187321303No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4200.11360839187321303No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4170.11279690335983293No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT4160.11252640718870624No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4130.11171491867532615No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4080.11036243781969265No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3980.10765747610842569No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC3970.107386979937299No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3920.1060344990816655No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.1057640029105388No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3840.10387052971265191No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3800.10278854502814513No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA3720.10062457565913155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAA200.001840321637.011
AAGCCCA200.001840321637.06
CAATCGT200.001840321637.015
GCCCCCT250.005491580329.637
CGGCCCC250.005491580329.635
ATATCTC250.005491580329.621
TATCTCA250.005491580329.622
GCGGCCC250.005491580329.634
TACACTT250.005491580329.65
ACACGAA453.9985935E-628.77777717
AAACACG453.9985935E-628.77777715
TGGACGT509.069319E-625.90000226
GCTCTCT509.069319E-625.90000230
GGACGTA509.069319E-625.90000227
AAGTGGA509.069319E-625.90000223
GCTTGTC601.3333101E-624.66666611
CGCTCTC601.3333101E-624.66666629
ACTGTGC451.3208413E-424.6666668
CCCTTGC701.915596E-723.78571332
GGTATCA6500.023.6230771
Produced by FastQC (version 0.11.5)