##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633481.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369691
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.219034274569843	33.0	33.0	33.0	14.0	33.0
2	30.791063347498316	33.0	33.0	33.0	27.0	33.0
3	30.59533502303275	33.0	33.0	33.0	27.0	33.0
4	30.933639174337486	33.0	33.0	33.0	27.0	33.0
5	31.070269495335292	33.0	33.0	33.0	27.0	33.0
6	32.85313410388676	37.0	33.0	37.0	14.0	37.0
7	33.13667089542348	37.0	33.0	37.0	14.0	37.0
8	33.31846596211431	37.0	33.0	37.0	27.0	37.0
9	33.264334268348435	37.0	33.0	37.0	27.0	37.0
10	33.10345396560912	37.0	33.0	37.0	14.0	37.0
11	33.21477125491289	37.0	33.0	37.0	27.0	37.0
12	33.33004860816195	37.0	33.0	37.0	27.0	37.0
13	33.38796454336189	37.0	33.0	37.0	27.0	37.0
14	33.46724967608083	37.0	33.0	37.0	27.0	37.0
15	33.539631746512626	37.0	33.0	37.0	27.0	37.0
16	33.53202809914226	37.0	33.0	37.0	27.0	37.0
17	33.49688253162776	37.0	33.0	37.0	27.0	37.0
18	33.47334936473974	37.0	33.0	37.0	27.0	37.0
19	33.43200402498302	37.0	33.0	37.0	27.0	37.0
20	33.40103762331244	37.0	33.0	37.0	27.0	37.0
21	33.41630713217254	37.0	33.0	37.0	27.0	37.0
22	33.251729146773926	37.0	33.0	37.0	22.0	37.0
23	33.31932343497678	37.0	33.0	37.0	27.0	37.0
24	33.320859853228775	37.0	33.0	37.0	27.0	37.0
25	33.32148199442237	37.0	33.0	37.0	27.0	37.0
26	33.18178424684399	37.0	33.0	37.0	22.0	37.0
27	33.14218631235275	37.0	33.0	37.0	14.0	37.0
28	33.16909256649472	37.0	33.0	37.0	22.0	37.0
29	33.177358929484356	37.0	33.0	37.0	22.0	37.0
30	33.16864895277407	37.0	33.0	37.0	22.0	37.0
31	33.15925732571255	37.0	33.0	37.0	22.0	37.0
32	33.10147393363647	37.0	33.0	37.0	14.0	37.0
33	33.075609089753335	37.0	33.0	37.0	14.0	37.0
34	33.06453768146912	37.0	33.0	37.0	14.0	37.0
35	32.954359181045795	37.0	33.0	37.0	14.0	37.0
36	33.01039787281811	37.0	33.0	37.0	14.0	37.0
37	32.98634805824324	37.0	33.0	37.0	14.0	37.0
38	32.96606625533216	37.0	33.0	37.0	14.0	37.0
39	32.832911810133325	37.0	33.0	37.0	14.0	37.0
40	32.58095003665223	37.0	33.0	37.0	14.0	37.0
41	32.795569813709285	37.0	33.0	37.0	14.0	37.0
42	32.77668647600293	37.0	33.0	37.0	14.0	37.0
43	32.24372516507029	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	18.0
16	46.0
17	40.0
18	63.0
19	142.0
20	346.0
21	719.0
22	1584.0
23	2747.0
24	4411.0
25	6602.0
26	9102.0
27	12129.0
28	15100.0
29	18525.0
30	22325.0
31	26742.0
32	32054.0
33	38744.0
34	49402.0
35	66152.0
36	62696.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81113416339592	22.562897122191234	12.690057372237895	22.935911342174954
2	17.975823052224698	23.752268786635327	35.7257818015586	22.546126359581383
3	19.254458453140597	28.527608191706044	29.334498270176983	22.883435084976373
4	13.217254409763829	20.92666578304584	38.33850431847137	27.51757548871896
5	14.869715519176827	37.63305030417294	34.46094170537016	13.03629247128007
6	26.968738757502887	41.739993670389595	17.290385754589643	14.000881817517874
7	26.682012816108585	32.72056934034099	22.64269349267361	17.954724350876813
8	24.615151572529488	34.969745003259476	19.92177250731016	20.49333091690087
9	26.8107689935649	14.16155654316713	20.297221192834016	38.730453270433955
10	17.010151721302385	26.832679183426162	32.48145072506499	23.675718370206468
11	34.614854026741256	23.789056265908556	23.268892128831915	18.327197578518277
12	23.34733601845866	27.294145651368307	28.431311554784944	20.927206775388093
13	29.3488345672467	23.069265954540413	24.76338347430692	22.818516003905962
14	22.334598353760303	21.583430486541463	28.37288438182157	27.709086777876657
15	25.694972287667266	27.56383033398162	25.5524208054835	21.18877657286761
16	22.13686565266669	27.513247549980928	25.745284575496836	24.60460222185555
17	22.495002583238435	27.43101671395841	25.433402490187753	24.6405782126154
18	22.787679440397522	26.15643875560941	28.53951002323562	22.516371780757442
19	26.389065462778373	25.355770089074387	28.179750115637113	20.07541433251012
20	25.060117774032907	24.629487869599206	28.964459508075663	21.345934848292224
21	23.351663957196685	26.79670319266631	28.60442910430603	21.24720374583098
22	22.87369722281581	26.616552742695927	27.897893105323096	22.611856929165167
23	22.7165389473912	26.485903092041735	29.51410772780511	21.283450232761954
24	22.488240178960268	27.612249148613298	26.39447538620091	23.505035286225525
25	23.083872747781257	27.136716879772564	28.627691775022925	21.15171859742325
26	23.06385603111788	27.23274302052254	28.32662953655891	21.376771411800664
27	23.48637105041778	26.486173588212857	27.599806324741472	22.427649036627887
28	21.94995279841814	26.717447814526185	29.210070031458706	22.12252935559697
29	24.70279233197454	26.57949476725157	26.417197064575547	22.30051583619834
30	23.95514091498035	25.60381507799757	28.581977922102514	21.85906608491957
31	23.57806925242973	26.07501940810028	27.875712419290704	22.471198920179287
32	21.404362021255587	26.11911028399393	28.75347249459684	23.72305520015364
33	21.152530085936633	26.87677005931981	29.456221547183997	22.514478307559557
34	22.391943542039165	26.331449778328388	28.513271894636333	22.76333478499612
35	23.111733853407305	27.018239556819072	27.66905334454991	22.200973245223715
36	23.904558130979655	26.75721075168181	27.092355507707786	22.245875609630748
37	23.803122066807145	25.88729506533835	27.290899697314785	23.018683170539724
38	23.183415338755882	26.65523369516705	27.112101728200038	23.04924923787704
39	22.808778141745403	26.519174121090316	27.959025239997725	22.713022497166552
40	22.686513872396137	27.912770394735066	28.08778141745404	21.312934315414765
41	23.33976212566711	25.681447479110936	27.576002661682324	23.402787733539633
42	21.763580936511843	27.900327570863237	27.788883148359034	22.547208344265886
43	23.26916262500304	26.031199028377756	27.29495713988169	23.404681206737518
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	21.5
2	23.0
3	106.5
4	190.0
5	190.0
6	288.0
7	386.0
8	444.0
9	502.0
10	680.5
11	859.0
12	859.0
13	1314.5
14	1770.0
15	3254.0
16	4738.0
17	4646.5
18	4555.0
19	4555.0
20	3728.0
21	2901.0
22	2087.0
23	1273.0
24	1450.5
25	1628.0
26	1628.0
27	2248.5
28	2869.0
29	3783.0
30	4697.0
31	6539.5
32	8382.0
33	8382.0
34	12799.5
35	17217.0
36	18788.5
37	20360.0
38	22574.5
39	24789.0
40	24789.0
41	28033.0
42	31277.0
43	33183.5
44	35090.0
45	35126.0
46	35162.0
47	35162.0
48	35067.5
49	34973.0
50	34351.5
51	33730.0
52	32230.0
53	30730.0
54	30730.0
55	28784.0
56	26838.0
57	21768.0
58	16698.0
59	14478.5
60	12259.0
61	12259.0
62	10269.0
63	8279.0
64	5922.0
65	3565.0
66	2811.0
67	2057.0
68	2057.0
69	1552.5
70	1048.0
71	752.0
72	456.0
73	368.5
74	281.0
75	281.0
76	166.0
77	51.0
78	37.0
79	23.0
80	13.5
81	4.0
82	4.0
83	6.0
84	8.0
85	4.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	369691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.835384145137425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.49491976453511	20.259351728876275
2	8.891420046770422	4.7720934510172
3	3.6811547455850335	2.9635560508641
4	2.192363519071043	2.3533166888022703
5	1.4293202161116039	1.9178178532882866
6	1.0704781872429643	1.723601602419318
7	0.816466413998871	1.5337132902883759
8	0.6350294331102331	1.3633007024785564
9	0.5392710265301186	1.3024390639750494
>10	4.239577453431175	22.953764089469313
>50	0.5584227078461415	10.570449375289092
>100	0.4283928715426175	22.682456429829237
>500	0.019151681316022903	3.3790381697147076
>1k	0.004031932908636401	2.225101503688215
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2811	0.7603647370371472	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2586	0.6995030985336403	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1506	0.4073672337168067	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1323	0.35786643440062105	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	847	0.22911025694431295	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	803	0.21720842541473828	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	787	0.2128804866767111	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	767	0.20747056325417712	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	710	0.19205228149995537	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	702	0.1898883121309418	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	698	0.188806327446435	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	687	0.18583086956404132	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	669	0.18096193848376077	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	646	0.17474052654784672	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	628	0.16987159546756617	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	628	0.16987159546756617	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	600	0.16229770267601862	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	588	0.15905174862249824	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	562	0.1520188481732041	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	550	0.14877289411968372	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	550	0.14877289411968372	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	537	0.14525644389503667	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	533	0.14417445921052988	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	496	0.13416610087884207	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	484	0.1309201468253217	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	482	0.13037915448306828	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	474	0.12821518511405472	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	470	0.12713320042954793	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	469	0.12686270425842122	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	456	0.12334625403377417	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	455	0.12307575786264745	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	454	0.12280526169152076	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	450	0.12172327700701395	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	447	0.12091178849363386	No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGC	446	0.12064129232250717	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	445	0.12037079615138048	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	442	0.11955930763800039	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	441	0.11928881146687367	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	439	0.11874781912462029	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	438	0.11847732295349359	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	435	0.1176658344401135	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	435	0.1176658344401135	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	433	0.11712484209786012	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	430	0.11631335358448001	No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT	425	0.11496087272884652	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	424	0.11469037655771983	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	420	0.11360839187321303	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	420	0.11360839187321303	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	417	0.11279690335983293	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	416	0.11252640718870624	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	413	0.11171491867532615	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	408	0.11036243781969265	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	398	0.10765747610842569	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	397	0.107386979937299	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	392	0.1060344990816655	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	391	0.1057640029105388	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	384	0.10387052971265191	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	380	0.10278854502814513	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	372	0.10062457565913155	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.704961711266977E-4	0.0	0.0
2	0.0	0.0	2.704961711266977E-4	0.0	0.0
3	0.0	0.0	8.114885133800931E-4	0.0	0.0
4	0.0	0.0	0.001081984684506791	0.0	0.0
5	0.0	0.0	0.001081984684506791	0.0	0.0
6	2.704961711266977E-4	0.0	0.0016229770267601861	0.0	0.0
7	2.704961711266977E-4	0.0	0.0040574425669004655	0.0	0.0
8	2.704961711266977E-4	0.0	0.0064919081070407446	0.0	0.0
9	2.704961711266977E-4	0.0	0.007844388962674233	5.409923422533955E-4	0.0
10	2.704961711266977E-4	0.0	0.010549350673941211	5.409923422533955E-4	0.0
11	5.409923422533955E-4	0.0	0.013795304727461584	5.409923422533955E-4	0.0
12	5.409923422533955E-4	0.0	0.014877289411968373	5.409923422533955E-4	0.0
13	5.409923422533955E-4	0.0	0.016229770267601862	5.409923422533955E-4	0.0
14	5.409923422533955E-4	0.0	0.01758225112323535	5.409923422533955E-4	0.0
15	5.409923422533955E-4	0.0	0.018664235807742142	5.409923422533955E-4	0.0
16	5.409923422533955E-4	0.0	0.019475724321122233	5.409923422533955E-4	0.0
17	5.409923422533955E-4	0.0	0.02001671666337563	5.409923422533955E-4	0.0
18	8.114885133800931E-4	0.0	0.020557709005629025	5.409923422533955E-4	0.0
19	8.114885133800931E-4	0.0	0.021098701347882422	5.409923422533955E-4	0.0
20	8.114885133800931E-4	0.0	0.02136919751900912	8.114885133800931E-4	0.0
21	8.114885133800931E-4	0.0	0.02245118220351591	8.114885133800931E-4	0.0
22	8.114885133800931E-4	0.0	0.022992174545769305	0.001081984684506791	0.0
23	8.114885133800931E-4	0.0	0.023262670716896004	0.0018934731978868839	0.0
24	8.114885133800931E-4	0.0	0.023262670716896004	0.0032459540535203723	0.0
25	8.114885133800931E-4	0.0	0.023262670716896004	0.00351645022464707	0.0
26	8.114885133800931E-4	0.0	0.023262670716896004	0.004868931080280558	0.0
27	0.001081984684506791	0.0	0.023262670716896004	0.00703290044929414	0.0
28	0.001081984684506791	0.0	0.023262670716896004	0.021910189861262513	0.0
29	0.001081984684506791	0.0	0.023262670716896004	0.05058278400069247	0.0
30	0.001081984684506791	0.0	0.0235331668880227	0.0827718283647695	0.0
31	0.001081984684506791	0.0	0.023803663059149396	0.16256819884714532	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACAA	20	0.0018403216	37.0	11
AAGCCCA	20	0.0018403216	37.0	6
CAATCGT	20	0.0018403216	37.0	15
GCCCCCT	25	0.0054915803	29.6	37
CGGCCCC	25	0.0054915803	29.6	35
ATATCTC	25	0.0054915803	29.6	21
TATCTCA	25	0.0054915803	29.6	22
GCGGCCC	25	0.0054915803	29.6	34
TACACTT	25	0.0054915803	29.6	5
ACACGAA	45	3.9985935E-6	28.777777	17
AAACACG	45	3.9985935E-6	28.777777	15
TGGACGT	50	9.069319E-6	25.900002	26
GCTCTCT	50	9.069319E-6	25.900002	30
GGACGTA	50	9.069319E-6	25.900002	27
AAGTGGA	50	9.069319E-6	25.900002	23
GCTTGTC	60	1.3333101E-6	24.666666	11
CGCTCTC	60	1.3333101E-6	24.666666	29
ACTGTGC	45	1.3208413E-4	24.666666	8
CCCTTGC	70	1.915596E-7	23.785713	32
GGTATCA	650	0.0	23.623077	1
>>END_MODULE