FastQCFastQC Report
Fri 10 Feb 2017
ERR1633480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913411
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58590.6414418044013046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40910.44788162174530416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23020.2520223645215571No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12760.13969614992593696No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12090.13236100725741207No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10980.1202087559707514No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.11747176243771972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.025.9143981
GACGGAC502.7015136E-422.27
GTATCAA31500.021.4365081
GTGTATC450.003825216320.5555551
CCACGTT555.141981E-420.1818237
GTACTGG4650.018.6989251
TGCGGGT1306.9485395E-1018.521
TGTACGC500.007033802618.534
CGCTTCA500.007033802618.532
GAAAGCG1451.546141E-1017.86206834
TATAACT2200.017.6590924
ACTATCG4450.017.46067411
GGTCGGC852.722124E-517.41176422
GTCGCCC2450.017.36734637
TTGTCCG752.0666813E-417.26666613
TCGGCTC752.0666813E-417.26666624
AAAGCGT1401.8681021E-917.17857235
GTCGGCT650.001579598217.07692323
ACGGTAC1107.8032826E-716.8181843
TCTGCGG1554.0017767E-1016.70967919
Produced by FastQC (version 0.11.5)