##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633480.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	913411
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.883801486953846	33.0	33.0	33.0	33.0	33.0
2	31.998986217595366	33.0	33.0	33.0	33.0	33.0
3	31.710187418369166	33.0	33.0	33.0	27.0	33.0
4	31.993104965891586	33.0	33.0	33.0	33.0	33.0
5	32.10050459212775	33.0	33.0	33.0	33.0	33.0
6	35.27806431058965	37.0	37.0	37.0	33.0	37.0
7	35.42640498089031	37.0	37.0	37.0	33.0	37.0
8	35.564270629541355	37.0	37.0	37.0	33.0	37.0
9	35.672093942376435	37.0	37.0	37.0	33.0	37.0
10	35.6754057045514	37.0	37.0	37.0	33.0	37.0
11	35.7008181421069	37.0	37.0	37.0	33.0	37.0
12	35.685027879016125	37.0	37.0	37.0	33.0	37.0
13	35.690083653470346	37.0	37.0	37.0	33.0	37.0
14	35.66555143303508	37.0	37.0	37.0	33.0	37.0
15	35.68508590327903	37.0	37.0	37.0	33.0	37.0
16	35.66073760880918	37.0	37.0	37.0	33.0	37.0
17	35.67112066747609	37.0	37.0	37.0	33.0	37.0
18	35.650309663448326	37.0	37.0	37.0	33.0	37.0
19	35.63924892518264	37.0	37.0	37.0	33.0	37.0
20	35.635157667249466	37.0	37.0	37.0	33.0	37.0
21	35.63510073778398	37.0	37.0	37.0	33.0	37.0
22	35.54338080009985	37.0	37.0	37.0	33.0	37.0
23	35.58830252755879	37.0	37.0	37.0	33.0	37.0
24	35.579227751800666	37.0	37.0	37.0	33.0	37.0
25	35.60663053105338	37.0	37.0	37.0	33.0	37.0
26	35.49719239203382	37.0	37.0	37.0	33.0	37.0
27	35.507649896924825	37.0	37.0	37.0	33.0	37.0
28	35.52253914174452	37.0	37.0	37.0	33.0	37.0
29	35.532070448023944	37.0	37.0	37.0	33.0	37.0
30	35.52927105103836	37.0	37.0	37.0	33.0	37.0
31	35.523881363373114	37.0	37.0	37.0	33.0	37.0
32	35.5094377011006	37.0	37.0	37.0	33.0	37.0
33	35.50881804576472	37.0	37.0	37.0	33.0	37.0
34	35.485952106992364	37.0	37.0	37.0	33.0	37.0
35	35.417696962265616	37.0	37.0	37.0	33.0	37.0
36	35.45103244870053	37.0	37.0	37.0	33.0	37.0
37	35.44274811667475	37.0	37.0	37.0	33.0	37.0
38	35.430443688547655	37.0	37.0	37.0	33.0	37.0
39	35.31526771628544	37.0	37.0	37.0	33.0	37.0
40	35.09995500382632	37.0	37.0	37.0	33.0	37.0
41	35.26895231171948	37.0	37.0	37.0	33.0	37.0
42	35.30671187450118	37.0	37.0	37.0	33.0	37.0
43	34.97418248740162	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	20.0
16	40.0
17	35.0
18	25.0
19	24.0
20	60.0
21	132.0
22	350.0
23	849.0
24	1810.0
25	3333.0
26	5591.0
27	8402.0
28	11981.0
29	16474.0
30	21664.0
31	27991.0
32	36084.0
33	48460.0
34	69264.0
35	141815.0
36	519001.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.912895728209975	20.769401726057602	13.836049708181749	23.481652837550676
2	15.853213942026098	21.991195639202942	39.459345245459055	22.696245173311905
3	17.80162489832069	27.91207900933972	30.43000357998754	23.856292512352052
4	11.946976771683284	18.086600664979947	40.894186735215584	29.072235828121183
5	13.49392551655279	37.404519980600185	35.379801644604676	13.721752858242347
6	28.139358952322667	41.08752795838894	17.530334099326588	13.242778989961804
7	25.846524729831366	32.89493995583587	23.582702638790202	17.675832675542555
8	24.462153400823944	35.34859991832811	20.91183486951657	19.277411811331373
9	26.099422932283495	15.276146225521698	20.635508002421695	37.98892283977312
10	14.955261103709065	28.138811553616062	33.74647338383269	23.159453958842185
11	33.094959443229826	24.493355127100504	24.431061154288706	17.98062427538096
12	23.059827394239832	26.26637953779843	30.373183594241805	20.300609473719934
13	27.998677484724837	22.93348777275509	26.299442419677447	22.76839232284262
14	21.922551841394508	21.588310191140682	28.788573818357783	27.700564149107027
15	23.715611044754223	28.934400833797707	25.505166896391657	21.84482122505641
16	22.115017226637296	28.894440728215447	25.79375549451452	23.19678655063274
17	22.1222428895645	27.643306244396005	26.674301053961468	23.560149812078024
18	22.81579705083473	26.90891613961294	28.78167659465454	21.493610214897785
19	24.858907983372216	26.32090044897642	28.34145855480173	20.478733012849638
20	24.404019658182353	26.168066730091933	28.286061805693162	21.14185180603255
21	22.85915102839795	27.10028672744252	28.385469410812874	21.655092833346654
22	22.212454196413226	27.13192637268437	28.500861058165494	22.154758372736914
23	22.3744842135687	26.770862185806827	28.890937376493163	21.963716224131304
24	22.278908399395235	27.180863817054973	27.929486288209798	22.610741495339994
25	22.565088443209028	27.271841482092945	28.530530068063552	21.632540006634475
26	22.71660840519766	27.305561242419895	28.270515682425547	21.707314669956897
27	22.762918335776554	27.05167772229588	28.031302447638577	22.154101494288987
28	22.15224033868653	27.073354711077492	28.522209607723138	22.252195342512845
29	23.201384699768234	27.030000733514264	27.882081560217692	21.88653300649981
30	23.052382771829986	26.701999428515748	28.49889042282171	21.746727376832553
31	22.649716283250363	26.92993624994663	28.25891082984549	22.161436636957514
32	21.394421569260714	26.91449960642033	28.825140051959085	22.86593877235987
33	21.555356789002982	27.084521644692256	29.21959555993961	22.140526006365153
34	22.337151621778148	26.59142488978127	28.709310485641183	22.362113002799397
35	22.25099106535831	27.412413469949453	28.103121157945328	22.23347430674691
36	22.756240071555958	27.09689285546156	28.213476737197162	21.93339033578531
37	22.735110481480955	26.522562132490197	28.22792806305157	22.51439932297728
38	22.727446899588465	26.86348204696462	28.023638865746086	22.385432187700825
39	22.297957874385137	26.784656633213306	28.503707531439844	22.413677960961714
40	22.23183211062709	27.436608492781456	28.432874138804987	21.898685257786475
41	22.25077210587567	26.335789693796112	28.52604139866938	22.887396801658838
42	21.73950171390535	27.384167696688564	28.558118962876517	22.318211626529568
43	22.540893420377024	26.541611607480093	28.31934364705483	22.598151325088047
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	71.0
2	92.0
3	337.5
4	583.0
5	583.0
6	757.0
7	931.0
8	852.0
9	773.0
10	1109.0
11	1445.0
12	1445.0
13	2198.0
14	2951.0
15	5291.5
16	7632.0
17	8968.0
18	10304.0
19	10304.0
20	9999.0
21	9694.0
22	10799.5
23	11905.0
24	14508.5
25	17112.0
26	17112.0
27	20484.5
28	23857.0
29	27149.5
30	30442.0
31	34495.5
32	38549.0
33	38549.0
34	44155.0
35	49761.0
36	53074.0
37	56387.0
38	58869.5
39	61352.0
40	61352.0
41	63378.0
42	65404.0
43	66692.5
44	67981.0
45	66834.0
46	65687.0
47	65687.0
48	65028.0
49	64369.0
50	64198.0
51	64027.0
52	62023.5
53	60020.0
54	60020.0
55	57291.0
56	54562.0
57	47852.5
58	41143.0
59	37476.5
60	33810.0
61	33810.0
62	29856.0
63	25902.0
64	21372.5
65	16843.0
66	14049.5
67	11256.0
68	11256.0
69	9488.0
70	7720.0
71	6448.5
72	5177.0
73	3844.5
74	2512.0
75	2512.0
76	1852.5
77	1193.0
78	936.5
79	680.0
80	555.5
81	431.0
82	431.0
83	376.5
84	322.0
85	298.5
86	275.0
87	219.0
88	163.0
89	163.0
90	122.0
91	81.0
92	53.0
93	25.0
94	16.5
95	8.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	913411.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.8109913947442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.92099498811477	48.51146903304277
2	7.671361751705279	8.562926094207864
3	1.8335088872885894	3.0698984619195144
4	0.8207533517093514	1.8322823299783229
5	0.47230077448629476	1.317978723029281
6	0.33328898497994286	1.116071320360717
7	0.25155649468650954	0.9827732152168561
8	0.19203652934413187	0.857419926936151
9	0.14325573935404606	0.7195720352702745
>10	1.1097908153122502	12.389833902098934
>50	0.1410127332575574	5.562817409652355
>100	0.10257063134204894	10.906914992574453
>500	0.006174154499930314	2.2906055429979215
>1k	0.0011949976451478027	1.2281700705868897
>5k	1.9916627419130044E-4	0.6512669421276664
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5859	0.6414418044013046	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4091	0.44788162174530416	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2302	0.2520223645215571	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1276	0.13969614992593696	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1209	0.13236100725741207	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1098	0.1202087559707514	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1073	0.11747176243771972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.189594826425344E-4	0.0	0.0
2	0.0	0.0	2.189594826425344E-4	0.0	0.0
3	0.0	0.0	2.189594826425344E-4	0.0	0.0
4	0.0	0.0	2.189594826425344E-4	3.2843922396380164E-4	0.0
5	0.0	0.0	6.568784479276033E-4	3.2843922396380164E-4	0.0
6	0.0	0.0	0.001532716378497741	3.2843922396380164E-4	0.0
7	0.0	0.0	0.0031749124983167488	3.2843922396380164E-4	0.0
8	0.0	0.0	0.003612831463601818	3.2843922396380164E-4	0.0
9	0.0	0.0	0.0038317909462443523	5.47398706606336E-4	0.0
10	3.2843922396380164E-4	0.0	0.004269709911529421	6.568784479276033E-4	0.0
11	3.2843922396380164E-4	0.0	0.006130865513990964	6.568784479276033E-4	0.0
12	3.2843922396380164E-4	0.0	0.006568784479276032	6.568784479276033E-4	0.0
13	3.2843922396380164E-4	0.0	0.006897223703239834	6.568784479276033E-4	0.0
14	4.379189652850688E-4	0.0	0.007335142668524903	6.568784479276033E-4	0.0
15	4.379189652850688E-4	0.0	0.007554102151167437	6.568784479276033E-4	0.0
16	4.379189652850688E-4	0.0	0.008101500857773773	7.663581892488705E-4	0.0
17	4.379189652850688E-4	0.0	0.008101500857773773	8.758379305701376E-4	0.0
18	4.379189652850688E-4	0.0	0.00821098059909504	0.001094797413212672	0.0
19	4.379189652850688E-4	0.0	0.008429940081737575	0.001094797413212672	0.0
20	4.379189652850688E-4	0.0	0.008539419823058843	0.0014232366371764737	0.0
21	4.379189652850688E-4	0.0	0.009086818529665178	0.001642196119819008	0.0
22	4.379189652850688E-4	0.0	0.009524737494950248	0.002189594826425344	0.0
23	4.379189652850688E-4	0.0	0.009524737494950248	0.00273699353303168	0.0
24	4.379189652850688E-4	0.0	0.009634217236271515	0.0031749124983167488	0.0
25	4.379189652850688E-4	0.0	0.009634217236271515	0.004050750428886887	0.0
26	4.379189652850688E-4	0.0	0.009634217236271515	0.005036068100778291	0.0
27	4.379189652850688E-4	0.0	0.009634217236271515	0.007225662927203636	0.0
28	4.379189652850688E-4	0.0	0.009634217236271515	0.01959687369650683	0.0
29	4.379189652850688E-4	0.0	0.009634217236271515	0.045215133165683354	0.0
30	4.379189652850688E-4	0.0	0.009634217236271515	0.07740217711413591	0.0
31	4.379189652850688E-4	0.0	0.009634217236271515	0.14265210294161118	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1285	0.0	25.914398	1
GACGGAC	50	2.7015136E-4	22.2	7
GTATCAA	3150	0.0	21.436508	1
GTGTATC	45	0.0038252163	20.555555	1
CCACGTT	55	5.141981E-4	20.18182	37
GTACTGG	465	0.0	18.698925	1
TGCGGGT	130	6.9485395E-10	18.5	21
TGTACGC	50	0.0070338026	18.5	34
CGCTTCA	50	0.0070338026	18.5	32
GAAAGCG	145	1.546141E-10	17.862068	34
TATAACT	220	0.0	17.659092	4
ACTATCG	445	0.0	17.460674	11
GGTCGGC	85	2.722124E-5	17.411764	22
GTCGCCC	245	0.0	17.367346	37
TTGTCCG	75	2.0666813E-4	17.266666	13
TCGGCTC	75	2.0666813E-4	17.266666	24
AAAGCGT	140	1.8681021E-9	17.178572	35
GTCGGCT	65	0.0015795982	17.076923	23
ACGGTAC	110	7.8032826E-7	16.818184	3
TCTGCGG	155	4.0017767E-10	16.709679	19
>>END_MODULE