FastQCFastQC Report
Fri 10 Feb 2017
ERR1633479.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633479.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634464
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33510.5281623543652595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23620.37228274575074394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13670.21545745700307664No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC10550.16628209007918496No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9420.14847178090482677No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8490.13381373884097444No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8440.13302567206334798No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8290.13066147173046855No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7280.11474252282241389No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7100.11190548242295859No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.10370958793564332No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6570.10355197458011803No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6480.10213345438039038No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6460.10181822766933979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.025.5398241
TTCTGCG601.3358294E-624.66666818
GTATCAA15500.023.6322571
TGACGAG400.001930238923.12530
AGCTAGT502.7002543E-422.1999996
TGCGGGT603.7228994E-521.58333421
GACCGTT450.003823946720.5555577
TTTGGCG450.003823946720.55555722
TGGCGTA555.1396043E-420.18181824
GGGGCGA555.1396043E-420.18181830
GCGGGTA656.895904E-519.92307722
ATTACCC2600.019.2115383
CCGCTCT902.149125E-618.528
CGGGATA609.2305976E-418.516
GGGTTAT902.149125E-618.51
CGCCTAT500.007031494818.49999834
TACTGAC500.007031494818.4999987
CCGTTTA500.007031494818.49999827
GTATTAG2800.017.8392851
ATAGGCG1156.391565E-817.69565232
Produced by FastQC (version 0.11.5)