FastQCFastQC Report
Fri 10 Feb 2017
ERR1633478.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633478.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899478
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91881.0214813480707698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66520.7395400443368265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35470.3943398282114738No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16340.1816609188885109No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14700.1634281216438868No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11250.12507254207440316No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.10150331636793784No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9100.10116978958907277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGT358.8678894E-426.42857210
GGTATCA15350.026.1530931
CGTGTCG451.3228998E-424.6666669
TACACCG400.001930862423.1255
GTATCAA39150.022.5874821
GTTATCG450.003825171220.55555511
ATAATAC1306.9485395E-1018.53
GACGTAT609.2346856E-418.528
CGTAGTA609.2346856E-418.52
ACGTATA609.2346856E-418.529
GGACCGT1700.018.56
ATCGGCG609.2346856E-418.512
CGCCGTT1103.8487997E-818.525
TAGGACC1601.8189894E-1218.54
ACGGTGT609.2346856E-418.535
ATTACGC500.007033722518.4999983
AGTACGT500.007033722518.4999985
CTGTGCG500.007033722518.4999989
GAGTCGC1351.1514203E-917.81481622
GACCGTT1156.400296E-817.6956527
Produced by FastQC (version 0.11.5)