FastQCFastQC Report
Fri 10 Feb 2017
ERR1633477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509177
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31780.6241444527148712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23590.46329665322667757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12180.23920954795680088No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.13256686771004975No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5780.11351651783171667No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCA358.862584E-426.4285720
GGTATCA6000.024.6666661
GACCGAG400.001929717623.12500226
CCGTGTC400.001929717623.1250029
GTATCAA15600.022.6506421
TTCGCCG502.699238E-422.224
GAGACCG450.003822925520.55555524
TGCGGGT609.227184E-418.521
TGCGTGG500.00702963118.510
TAGTACC500.00702963118.54
ATATAGC953.5984103E-617.5263166
TGGACCG650.001578300817.0769215
GCCGCTC650.001578300817.07692127
TCTAATA1301.387707E-817.0769212
GGACCGT1005.864862E-616.6500026
CTATAAG904.4381355E-516.4444451
TGCTCGC904.4381355E-516.44444510
CCGGCAG700.002589835315.85714216
GCCGGCA700.002589835315.85714215
TCTGGGG1059.32259E-615.8571423
Produced by FastQC (version 0.11.5)