##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633477.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	509177
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.931202705542475	33.0	33.0	33.0	33.0	33.0
2	32.04136675458632	33.0	33.0	33.0	33.0	33.0
3	31.763052926585452	33.0	33.0	33.0	33.0	33.0
4	32.02201591980785	33.0	33.0	33.0	33.0	33.0
5	32.12300437765256	33.0	33.0	33.0	33.0	33.0
6	35.3109900879262	37.0	37.0	37.0	33.0	37.0
7	35.458492822731586	37.0	37.0	37.0	33.0	37.0
8	35.58943353686439	37.0	37.0	37.0	33.0	37.0
9	35.6940395972324	37.0	37.0	37.0	33.0	37.0
10	35.71309387501792	37.0	37.0	37.0	33.0	37.0
11	35.725346981501524	37.0	37.0	37.0	33.0	37.0
12	35.72169402781351	37.0	37.0	37.0	33.0	37.0
13	35.71899555557301	37.0	37.0	37.0	33.0	37.0
14	35.70274383956856	37.0	37.0	37.0	33.0	37.0
15	35.71089031908354	37.0	37.0	37.0	33.0	37.0
16	35.70682493514043	37.0	37.0	37.0	33.0	37.0
17	35.69602908222485	37.0	37.0	37.0	33.0	37.0
18	35.67589266600809	37.0	37.0	37.0	33.0	37.0
19	35.66958641101228	37.0	37.0	37.0	33.0	37.0
20	35.66929574588011	37.0	37.0	37.0	33.0	37.0
21	35.666389094558475	37.0	37.0	37.0	33.0	37.0
22	35.56667327864377	37.0	37.0	37.0	33.0	37.0
23	35.62107282929119	37.0	37.0	37.0	33.0	37.0
24	35.600875530512965	37.0	37.0	37.0	33.0	37.0
25	35.630046133269964	37.0	37.0	37.0	33.0	37.0
26	35.52485481473044	37.0	37.0	37.0	33.0	37.0
27	35.54735975898362	37.0	37.0	37.0	33.0	37.0
28	35.55684761880446	37.0	37.0	37.0	33.0	37.0
29	35.565015701808996	37.0	37.0	37.0	33.0	37.0
30	35.56723104146495	37.0	37.0	37.0	33.0	37.0
31	35.560406302719876	37.0	37.0	37.0	33.0	37.0
32	35.54483018675235	37.0	37.0	37.0	33.0	37.0
33	35.52718602764854	37.0	37.0	37.0	33.0	37.0
34	35.50689445909772	37.0	37.0	37.0	33.0	37.0
35	35.45928233207706	37.0	37.0	37.0	33.0	37.0
36	35.46491495098954	37.0	37.0	37.0	33.0	37.0
37	35.4697777000925	37.0	37.0	37.0	33.0	37.0
38	35.45033652344863	37.0	37.0	37.0	33.0	37.0
39	35.341050361662056	37.0	37.0	37.0	33.0	37.0
40	35.135336042279995	37.0	37.0	37.0	33.0	37.0
41	35.292340384581394	37.0	37.0	37.0	33.0	37.0
42	35.333188655418446	37.0	37.0	37.0	33.0	37.0
43	34.99179067396996	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	13.0
16	26.0
17	32.0
18	21.0
19	19.0
20	25.0
21	57.0
22	183.0
23	487.0
24	988.0
25	1833.0
26	3075.0
27	4446.0
28	6615.0
29	8871.0
30	11797.0
31	15274.0
32	19537.0
33	26222.0
34	38246.0
35	78502.0
36	292906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.711972457514776	21.433803962079985	14.378104274152209	23.476119306253032
2	15.254616763915102	22.805429153319963	39.813856478199135	22.1260976045658
3	17.53162456277483	27.91544001398335	31.399493692763023	23.15344173047879
4	12.022734726823876	18.316813210337465	41.03779235904214	28.622659703796522
5	13.030439316779823	37.93867358502053	35.66520090263307	13.36568619556657
6	27.614955113840573	41.326493537610695	17.909685629947937	13.1488657186008
7	24.69180658199408	33.57241195105042	24.167234576581425	17.568546890374073
8	24.01954526618445	35.766344512811855	21.606435483142405	18.607674737861295
9	25.830114086064377	15.360670258083143	21.078917547336193	37.730298108516294
10	14.215881707147023	28.656636101002203	34.85978353303468	22.26769865881609
11	32.54742456945227	24.384251448906767	25.118770093700228	17.949553887940738
12	21.858607124830854	26.571702963802373	31.727081152526527	19.842608758840246
13	27.764804773192818	23.10493207666489	27.08331287548338	22.04695027465891
14	21.534358386180052	21.980175852404958	29.462053470600598	27.02341229081439
15	23.025391956038863	29.416882537899397	25.83247083037922	21.725254675682525
16	21.508826989435896	29.486406495187335	26.322084461788336	22.682682053588437
17	21.195576390921037	28.227315844981216	27.826080125378798	22.751027638718952
18	22.207994469506676	27.40559766053848	29.23600241173502	21.150405458219833
19	23.681156061644575	26.9961133358341	29.34068113838606	19.98204946413526
20	23.812151766478063	26.63769180461804	28.884651113463487	20.66550531544041
21	22.25846807691628	27.703922211725978	28.608912028626587	21.42869768273115
22	21.582082458555668	27.599047089715363	29.006219841037595	21.81265061069137
23	21.56499606227304	27.4014733579875	29.31632811379933	21.717202465940137
24	21.897886196744942	27.46431987305004	28.721642965020024	21.916150965184993
25	22.014348644970216	27.488280106917635	29.093615776046445	21.403755472065704
26	21.96721375867331	27.551715808058887	28.86304762391074	21.61802280935706
27	22.005903644508688	27.594922787164382	28.736765407706947	21.662408160619982
28	21.557925829328507	27.76126965672055	28.945337279570758	21.735467234380184
29	22.124526441689234	27.656001743990792	28.54802946715975	21.671442347160223
30	22.030256669095422	27.71649151473849	29.01780716725225	21.235444648913834
31	22.190122491785765	27.369264519017943	28.90095192830784	21.539661060888456
32	21.116232665654575	27.4293614990465	29.22107636440766	22.23332947089126
33	21.327357677192804	27.28776044479621	29.72954395033554	21.655337927675443
34	21.518450362054846	27.352178122735317	29.01643239973526	22.112939115474582
35	21.638840717471528	27.925456177321443	28.864618786787304	21.571084318419725
36	22.009635156340526	27.558982436362996	28.85420983273007	21.577172574566408
37	22.27712563607547	27.11827124948692	28.68138191630808	21.923221198129532
38	22.14357679156757	27.41993452178712	28.682167497746363	21.75432118889895
39	21.866855729932812	27.3702464958158	28.896042044318577	21.866855729932812
40	21.62764618197601	27.70667174675997	29.118361591352322	21.5473204799117
41	21.645910950416063	27.072511130707007	29.26978241358113	22.0117955052958
42	21.52021792029098	27.613383950964003	28.987562281878404	21.87883584686661
43	21.615076878963503	27.24691021000556	28.86226204247246	22.27575086855848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	56.0
2	54.0
3	227.5
4	401.0
5	401.0
6	472.0
7	543.0
8	504.5
9	466.0
10	656.5
11	847.0
12	847.0
13	1368.0
14	1889.0
15	3334.5
16	4780.0
17	5506.0
18	6232.0
19	6232.0
20	6193.5
21	6155.0
22	7121.0
23	8087.0
24	10111.5
25	12136.0
26	12136.0
27	14217.5
28	16299.0
29	19065.5
30	21832.0
31	23547.0
32	25262.0
33	25262.0
34	27121.0
35	28980.0
36	30770.5
37	32561.0
38	33331.5
39	34102.0
40	34102.0
41	35091.5
42	36081.0
43	35628.0
44	35175.0
45	34945.0
46	34715.0
47	34715.0
48	34013.0
49	33311.0
50	33429.5
51	33548.0
52	33493.5
53	33439.0
54	33439.0
55	30914.5
56	28390.0
57	25311.5
58	22233.0
59	19561.0
60	16889.0
61	16889.0
62	14764.5
63	12640.0
64	10277.0
65	7914.0
66	6592.5
67	5271.0
68	5271.0
69	4359.0
70	3447.0
71	2904.5
72	2362.0
73	1877.5
74	1393.0
75	1393.0
76	1048.5
77	704.0
78	527.0
79	350.0
80	275.5
81	201.0
82	201.0
83	188.0
84	175.0
85	151.5
86	128.0
87	103.5
88	79.0
89	79.0
90	57.0
91	35.0
92	24.0
93	13.0
94	6.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	509177.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.2323000104837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.7030235092633	60.309405884222635
2	6.180852318490596	8.311058347945071
3	1.4043156981191376	2.8324612297613316
4	0.6479414908160819	1.7425038679914757
5	0.35950193424253696	1.2085070948671714
6	0.255038312625724	1.0288087409172126
7	0.18517550126828256	0.8714842399102615
8	0.13226021251441242	0.7113726629775446
9	0.10597172834304724	0.6412250729330309
>10	0.8941111666047318	12.152739593301527
>50	0.08757721326131801	4.0139625944937
>100	0.04275655063586572	4.588886747366646
>500	5.897455260119397E-4	0.24840692506374043
>1k	8.846182890179095E-4	1.3391769982486563
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3178	0.6241444527148712	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2359	0.46329665322667757	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1218	0.23920954795680088	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	675	0.13256686771004975	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	578	0.11351651783171667	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	1.9639535957044406E-4	0.0	0.0
5	0.0	0.0	1.9639535957044406E-4	0.0	0.0
6	0.0	0.0	0.0011783721574226643	1.9639535957044406E-4	0.0
7	0.0	0.0	0.002945930393556661	1.9639535957044406E-4	0.0
8	0.0	0.0	0.003338721112697549	1.9639535957044406E-4	0.0
9	0.0	0.0	0.004517093270120213	5.891860787113322E-4	0.0
10	1.9639535957044406E-4	0.0	0.005106279348831546	7.855814382817763E-4	0.0
11	1.9639535957044406E-4	0.0	0.007463023663676875	9.819767978522203E-4	0.0
12	1.9639535957044406E-4	0.0	0.008641395821099539	9.819767978522203E-4	0.0
13	1.9639535957044406E-4	0.0	0.009426977259381315	9.819767978522203E-4	0.0
14	1.9639535957044406E-4	0.0	0.009819767978522204	0.0011783721574226643	0.0
15	1.9639535957044406E-4	0.0	0.010212558697663092	0.0013747675169931085	0.0
16	1.9639535957044406E-4	0.0	0.010998140135944868	0.0013747675169931085	0.0
17	1.9639535957044406E-4	0.0	0.011980116933797089	0.0013747675169931085	0.0
18	1.9639535957044406E-4	0.0	0.011980116933797089	0.0013747675169931085	0.0
19	1.9639535957044406E-4	0.0	0.012372907652937977	0.0013747675169931085	0.0
20	1.9639535957044406E-4	0.0	0.01256930301250842	0.0017675582361339967	0.0
21	1.9639535957044406E-4	0.0	0.013354884450790196	0.002749535033986217	0.0
22	1.9639535957044406E-4	0.0	0.013944070529501529	0.002749535033986217	0.0
23	1.9639535957044406E-4	0.0	0.014336861248642417	0.002945930393556661	0.0
24	1.9639535957044406E-4	0.0	0.014533256608212862	0.0037315118318384374	0.0
25	1.9639535957044406E-4	0.0	0.014533256608212862	0.0037315118318384374	0.0
26	1.9639535957044406E-4	0.0	0.014533256608212862	0.004124302550979325	0.0
27	1.9639535957044406E-4	0.0	0.014533256608212862	0.005891860787113322	0.0
28	1.9639535957044406E-4	0.0	0.014533256608212862	0.017086396282628633	0.0
29	1.9639535957044406E-4	0.0	0.014533256608212862	0.03240523432912327	0.0
30	1.9639535957044406E-4	0.0	0.014533256608212862	0.05832942179242189	0.0
31	1.9639535957044406E-4	0.0	0.014533256608212862	0.11174895959558268	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGCA	35	8.862584E-4	26.42857	20
GGTATCA	600	0.0	24.666666	1
GACCGAG	40	0.0019297176	23.125002	26
CCGTGTC	40	0.0019297176	23.125002	9
GTATCAA	1560	0.0	22.650642	1
TTCGCCG	50	2.699238E-4	22.2	24
GAGACCG	45	0.0038229255	20.555555	24
TGCGGGT	60	9.227184E-4	18.5	21
TGCGTGG	50	0.007029631	18.5	10
TAGTACC	50	0.007029631	18.5	4
ATATAGC	95	3.5984103E-6	17.526316	6
TGGACCG	65	0.0015783008	17.076921	5
GCCGCTC	65	0.0015783008	17.076921	27
TCTAATA	130	1.387707E-8	17.076921	2
GGACCGT	100	5.864862E-6	16.650002	6
CTATAAG	90	4.4381355E-5	16.444445	1
TGCTCGC	90	4.4381355E-5	16.444445	10
CCGGCAG	70	0.0025898353	15.857142	16
GCCGGCA	70	0.0025898353	15.857142	15
TCTGGGG	105	9.32259E-6	15.857142	3
>>END_MODULE