FastQCFastQC Report
Fri 10 Feb 2017
ERR1633476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393400
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18450.46898830706659883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14080.35790543975597355No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG9360.23792577529232334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8920.22674123029994916No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG7160.18200305033045247No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT6700.170310116929334No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT5970.1517539400101678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACCG200.001840465137.030
GGTATAT250.005492003629.61
GACTGTT250.005492003629.67
TTAACGG453.999452E-628.77777735
CCGTACA405.930354E-527.7517
AAAGCGT358.858985E-426.42857235
GGTATCA3850.025.4675351
TTAGACT451.3210518E-424.6666664
GTCTAGA701.9162326E-723.7857151
GCTCCGA652.6754042E-622.7692329
AGGTCGC652.6754042E-622.7692335
TAAAGCC1700.021.7647064
TCTAGAC855.158836E-821.7647063
GTTAGGT603.7182726E-521.5833321
TAACGGC603.7182726E-521.58333236
AACGGCC603.7182726E-521.58333237
GTTCTAG957.1086106E-921.4210531
TAGAACT957.1086106E-921.4210534
TCCGAGG705.087859E-621.14285931
CTAGAAC806.936207E-720.81253
Produced by FastQC (version 0.11.5)