##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633476.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	393400
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.722155566853075	33.0	33.0	33.0	27.0	33.0
2	31.87156329435689	33.0	33.0	33.0	33.0	33.0
3	31.594865277071683	33.0	33.0	33.0	27.0	33.0
4	31.88318505338078	33.0	33.0	33.0	33.0	33.0
5	31.983309608540925	33.0	33.0	33.0	33.0	33.0
6	35.1110930350788	37.0	37.0	37.0	33.0	37.0
7	35.257859684799186	37.0	37.0	37.0	33.0	37.0
8	35.368022369089985	37.0	37.0	37.0	33.0	37.0
9	35.50178444331469	37.0	37.0	37.0	33.0	37.0
10	35.51772241992882	37.0	37.0	37.0	33.0	37.0
11	35.5396568378241	37.0	37.0	37.0	33.0	37.0
12	35.527031011692934	37.0	37.0	37.0	33.0	37.0
13	35.53231825114388	37.0	37.0	37.0	33.0	37.0
14	35.509494153533296	37.0	37.0	37.0	33.0	37.0
15	35.51113624809354	37.0	37.0	37.0	33.0	37.0
16	35.50033299440773	37.0	37.0	37.0	33.0	37.0
17	35.488965429588205	37.0	37.0	37.0	33.0	37.0
18	35.47840874428063	37.0	37.0	37.0	33.0	37.0
19	35.474039145907476	37.0	37.0	37.0	33.0	37.0
20	35.46711743772242	37.0	37.0	37.0	33.0	37.0
21	35.474717844433144	37.0	37.0	37.0	33.0	37.0
22	35.38570157600407	37.0	37.0	37.0	33.0	37.0
23	35.41251906456533	37.0	37.0	37.0	33.0	37.0
24	35.40580325368582	37.0	37.0	37.0	33.0	37.0
25	35.42833502796136	37.0	37.0	37.0	33.0	37.0
26	35.315582104728016	37.0	37.0	37.0	33.0	37.0
27	35.33540925266904	37.0	37.0	37.0	33.0	37.0
28	35.35447890188104	37.0	37.0	37.0	33.0	37.0
29	35.352089476359936	37.0	37.0	37.0	33.0	37.0
30	35.36942297915608	37.0	37.0	37.0	33.0	37.0
31	35.34944839857651	37.0	37.0	37.0	33.0	37.0
32	35.32280884595831	37.0	37.0	37.0	33.0	37.0
33	35.333863751906456	37.0	37.0	37.0	33.0	37.0
34	35.298523131672596	37.0	37.0	37.0	33.0	37.0
35	35.24570665988816	37.0	37.0	37.0	33.0	37.0
36	35.279641586171834	37.0	37.0	37.0	33.0	37.0
37	35.28679461108287	37.0	37.0	37.0	33.0	37.0
38	35.2722267412303	37.0	37.0	37.0	33.0	37.0
39	35.177486019318756	37.0	37.0	37.0	33.0	37.0
40	34.965205897305545	37.0	37.0	37.0	27.0	37.0
41	35.13150228774784	37.0	37.0	37.0	33.0	37.0
42	35.18713014743264	37.0	37.0	37.0	33.0	37.0
43	34.850523640061006	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	13.0
16	16.0
17	20.0
18	16.0
19	14.0
20	29.0
21	87.0
22	234.0
23	556.0
24	1059.0
25	1816.0
26	2942.0
27	4440.0
28	6107.0
29	8087.0
30	10377.0
31	13110.0
32	16643.0
33	22125.0
34	30939.0
35	62834.0
36	211936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97559735638027	20.223690899847483	13.365785460091509	24.43492628368073
2	16.427046263345197	21.579816980172854	37.754448398576514	24.23868835790544
3	18.581596339603458	25.649974580579567	29.951957295373667	25.816471784443312
4	13.024656837824097	17.13802745297407	38.830452465683784	31.006863243518048
5	14.253685815963395	36.67920691408236	34.86375190645653	14.203355363497714
6	30.655058464667007	39.51016776817489	16.342653787493646	13.492119979664462
7	27.482206405693947	31.715302491103202	22.4087442806304	18.393746822572446
8	25.500762582613117	35.25597356380275	20.115404168784952	19.127859684799187
9	25.55236400610066	15.14031520081342	20.13294356888663	39.17437722419928
10	14.77783426537875	28.240976105744792	33.43492628368073	23.54626334519573
11	34.487544483985765	23.327147941026944	23.803507880020337	18.381799694966954
12	23.51779359430605	25.604982206405698	30.095322826639553	20.781901372648704
13	28.609303507880018	22.34544992374174	25.919674631418406	23.125571936959837
14	22.677427554651754	20.536349771225215	27.20055922724962	29.58566344687341
15	23.743518047788513	29.095322826639553	24.41891204880529	22.74224707676665
16	23.521860701576003	28.18073207930859	24.43111337061515	23.866293848500252
17	22.323335027961363	27.197763091001526	26.19547534316218	24.283426537874934
18	23.97610574478902	26.473817996949673	27.476614133197764	22.073462125063546
19	25.05007625826131	25.892984239959326	26.965429588205385	22.09150991357397
20	24.025419420437213	26.763853584138282	27.27198779867819	21.938739196746315
21	23.254702592780884	26.509405185561768	28.041687849517032	22.194204372140316
22	22.859684799186578	26.283680732079308	27.776563294356887	23.080071174377224
23	23.09481443823081	26.2399593289273	27.603965429588207	23.061260803253685
24	22.82308083375699	27.04117946110829	26.450432130147433	23.68530757498729
25	23.475597356380273	27.04956786985257	27.185053380782918	22.28978139298424
26	23.797153024911033	26.122267412303003	27.314438230808335	22.76614133197763
27	23.156075241484494	26.614387391967465	27.03990849008643	23.189628876461615
28	22.978393492628367	25.844941535332993	27.95856634468734	23.218098627351296
29	23.494153533299443	26.026944585663447	26.994153533299443	23.48474834773767
30	23.108540925266905	26.488561260803255	27.56329435688866	22.83960345704118
31	23.22470767666497	26.45958312150483	27.663192679206915	22.652516522623284
32	22.9178952719878	26.471021860701576	27.543213014743262	23.06786985256736
33	23.289273004575495	26.121504829689883	28.00355871886121	22.58566344687341
34	23.5866802236909	25.942297915607526	27.390696492119982	23.080325368581597
35	23.108032536858158	26.874173868835786	27.370360955770206	22.647432638535843
36	23.75063548551093	25.738942552109812	27.831215048296897	22.679206914082357
37	23.680223690899847	25.94178952719878	26.995932892730046	23.382053889171328
38	23.575241484494153	26.322572445348246	27.451957295373663	22.650228774783933
39	23.298423995932893	26.22928317234367	27.25165226232842	23.22064056939502
40	22.84290798169802	27.045754956786983	27.47864768683274	22.632689374682258
41	23.782409761057448	25.78749364514489	27.266903914590745	23.163192679206915
42	22.906964921199798	26.455516014234874	27.289018810371125	23.348500254194203
43	23.544229791560753	25.96568378240976	27.410015251652265	23.080071174377224
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	47.5
2	57.0
3	136.5
4	216.0
5	216.0
6	276.5
7	337.0
8	268.5
9	200.0
10	296.0
11	392.0
12	392.0
13	618.0
14	844.0
15	1648.5
16	2453.0
17	2743.0
18	3033.0
19	3033.0
20	2991.0
21	2949.0
22	3019.5
23	3090.0
24	3929.5
25	4769.0
26	4769.0
27	6088.0
28	7407.0
29	8736.5
30	10066.0
31	11732.0
32	13398.0
33	13398.0
34	15953.0
35	18508.0
36	21198.5
37	23889.0
38	24604.5
39	25320.0
40	25320.0
41	26523.0
42	27726.0
43	29442.0
44	31158.0
45	30901.0
46	30644.0
47	30644.0
48	29883.5
49	29123.0
50	29692.0
51	30261.0
52	29499.5
53	28738.0
54	28738.0
55	26862.0
56	24986.0
57	21908.0
58	18830.0
59	17996.0
60	17162.0
61	17162.0
62	16480.0
63	15798.0
64	12287.5
65	8777.0
66	7096.5
67	5416.0
68	5416.0
69	4261.5
70	3107.0
71	2628.0
72	2149.0
73	1651.0
74	1153.0
75	1153.0
76	857.5
77	562.0
78	436.5
79	311.0
80	255.0
81	199.0
82	199.0
83	168.5
84	138.0
85	117.5
86	97.0
87	80.0
88	63.0
89	63.0
90	45.0
91	27.0
92	17.5
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	393400.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.450119766476924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.58411320641056	47.74783320843119
2	8.140547544582013	9.190697677127085
3	2.413381797752334	4.08707074576064
4	1.205312506403986	2.7216014137015
5	0.748512260272345	2.1126803369525113
6	0.4915234013184049	1.6647932923470035
7	0.34497298910926083	1.3631636585992122
8	0.24862631194828502	1.1227988069262513
9	0.19295968879977732	0.9803337788564591
>10	1.4188745659015112	15.766512388741209
>50	0.14078381833440748	5.286994389356621
>100	0.0671922769322949	6.132810280438039
>500	0.0022854515963365617	0.9833448324953453
>1k	9.141806385346246E-4	0.8393651902669531
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1845	0.46898830706659883	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1408	0.35790543975597355	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	936	0.23792577529232334	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	892	0.22674123029994916	No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG	716	0.18200305033045247	No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT	670	0.170310116929334	No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT	597	0.1517539400101678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.541942043721403E-4	0.0	0.0	0.0	0.0
3	2.541942043721403E-4	0.0	2.541942043721403E-4	0.0	0.0
4	2.541942043721403E-4	0.0	5.083884087442806E-4	0.0	0.0
5	2.541942043721403E-4	0.0	5.083884087442806E-4	0.0	0.0
6	2.541942043721403E-4	0.0	7.62582613116421E-4	0.0	0.0
7	2.541942043721403E-4	0.0	7.62582613116421E-4	0.0	0.0
8	2.541942043721403E-4	0.0	0.0010167768174885613	0.0	0.0
9	2.541942043721403E-4	0.0	0.0010167768174885613	2.541942043721403E-4	0.0
10	2.541942043721403E-4	0.0	0.0010167768174885613	5.083884087442806E-4	2.541942043721403E-4
11	2.541942043721403E-4	0.0	0.0012709710218607015	5.083884087442806E-4	2.541942043721403E-4
12	2.541942043721403E-4	0.0	0.0017793594306049821	5.083884087442806E-4	2.541942043721403E-4
13	2.541942043721403E-4	0.0	0.0017793594306049821	5.083884087442806E-4	2.541942043721403E-4
14	2.541942043721403E-4	0.0	0.0020335536349771225	5.083884087442806E-4	2.541942043721403E-4
15	2.541942043721403E-4	0.0	0.002287747839349263	5.083884087442806E-4	2.541942043721403E-4
16	2.541942043721403E-4	0.0	0.002287747839349263	7.62582613116421E-4	2.541942043721403E-4
17	2.541942043721403E-4	0.0	0.002287747839349263	7.62582613116421E-4	2.541942043721403E-4
18	2.541942043721403E-4	0.0	0.002287747839349263	0.0010167768174885613	2.541942043721403E-4
19	2.541942043721403E-4	0.0	0.002287747839349263	0.0010167768174885613	2.541942043721403E-4
20	2.541942043721403E-4	0.0	0.002541942043721403	0.0010167768174885613	2.541942043721403E-4
21	2.541942043721403E-4	0.0	0.002541942043721403	0.0012709710218607015	2.541942043721403E-4
22	2.541942043721403E-4	0.0	0.002541942043721403	0.0012709710218607015	2.541942043721403E-4
23	5.083884087442806E-4	0.0	0.002541942043721403	0.001525165226232842	2.541942043721403E-4
24	5.083884087442806E-4	0.0	0.0027961362480935434	0.0017793594306049821	2.541942043721403E-4
25	5.083884087442806E-4	0.0	0.0027961362480935434	0.002287747839349263	2.541942043721403E-4
26	5.083884087442806E-4	0.0	0.0027961362480935434	0.003050330452465684	2.541942043721403E-4
27	5.083884087442806E-4	0.0	0.0027961362480935434	0.004067107269954245	2.541942043721403E-4
28	5.083884087442806E-4	0.0	0.0027961362480935434	0.010421962379257754	2.541942043721403E-4
29	5.083884087442806E-4	0.0	0.0027961362480935434	0.018810371123538384	2.541942043721403E-4
30	5.083884087442806E-4	0.0	0.0027961362480935434	0.03457041179461108	2.541942043721403E-4
31	5.083884087442806E-4	0.0	0.0027961362480935434	0.0762582613116421	2.541942043721403E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACCG	20	0.0018404651	37.0	30
GGTATAT	25	0.0054920036	29.6	1
GACTGTT	25	0.0054920036	29.6	7
TTAACGG	45	3.999452E-6	28.777777	35
CCGTACA	40	5.930354E-5	27.75	17
AAAGCGT	35	8.858985E-4	26.428572	35
GGTATCA	385	0.0	25.467535	1
TTAGACT	45	1.3210518E-4	24.666666	4
GTCTAGA	70	1.9162326E-7	23.785715	1
GCTCCGA	65	2.6754042E-6	22.76923	29
AGGTCGC	65	2.6754042E-6	22.76923	35
TAAAGCC	170	0.0	21.764706	4
TCTAGAC	85	5.158836E-8	21.764706	3
GTTAGGT	60	3.7182726E-5	21.583332	1
TAACGGC	60	3.7182726E-5	21.583332	36
AACGGCC	60	3.7182726E-5	21.583332	37
GTTCTAG	95	7.1086106E-9	21.421053	1
TAGAACT	95	7.1086106E-9	21.421053	4
TCCGAGG	70	5.087859E-6	21.142859	31
CTAGAAC	80	6.936207E-7	20.8125	3
>>END_MODULE