FastQCFastQC Report
Fri 10 Feb 2017
ERR1633475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749442
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41400.5524109937793719No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30590.408170345403647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16570.22109783011894182No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8510.11355114872131533No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA8320.1110159291846467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.027.9555551
TATTGCG358.8665023E-426.4285729
CCGGTTC601.3363697E-624.66666821
GTATCAA19350.023.9974141
TTCGCCG851.9736035E-923.94117524
CCCGGTT701.9210711E-723.78571520
CGGTTCG652.6807775E-622.7692322
GTCGCCA1107.4578566E-1121.86363612
AGACCCG450.003824584720.5555555
GCCGGCA1101.7516868E-920.18181815
TTGGCCG1403.6379788E-1219.8214331
ATCGTAC759.258714E-619.73333425
GGTTCGC759.258714E-619.73333423
CGAACCG1800.019.52777731
TCGCCAT1306.9485395E-1018.513
CCGCTGA1002.8716022E-718.526
GTTCGCC801.6154469E-518.524
CTACGGT500.00703265418.527
CGGGATT902.1501837E-618.517
GGCCGCA1552.0008883E-1117.90322733
Produced by FastQC (version 0.11.5)