##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633475.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	749442
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.923068095996754	33.0	33.0	33.0	33.0	33.0
2	32.038307167199065	33.0	33.0	33.0	33.0	33.0
3	31.75605050157317	33.0	33.0	33.0	27.0	33.0
4	32.026871992762615	33.0	33.0	33.0	33.0	33.0
5	32.12903333413393	33.0	33.0	33.0	33.0	33.0
6	35.31757227377169	37.0	37.0	37.0	33.0	37.0
7	35.45783129314877	37.0	37.0	37.0	33.0	37.0
8	35.57542278121589	37.0	37.0	37.0	33.0	37.0
9	35.681341584805764	37.0	37.0	37.0	33.0	37.0
10	35.704223942613304	37.0	37.0	37.0	33.0	37.0
11	35.72266566325346	37.0	37.0	37.0	33.0	37.0
12	35.71030580084916	37.0	37.0	37.0	33.0	37.0
13	35.70481504906317	37.0	37.0	37.0	33.0	37.0
14	35.6900627400119	37.0	37.0	37.0	33.0	37.0
15	35.69744289751575	37.0	37.0	37.0	33.0	37.0
16	35.685129736523976	37.0	37.0	37.0	33.0	37.0
17	35.67286327694471	37.0	37.0	37.0	33.0	37.0
18	35.66333218581291	37.0	37.0	37.0	33.0	37.0
19	35.665505802984086	37.0	37.0	37.0	33.0	37.0
20	35.66142143087791	37.0	37.0	37.0	33.0	37.0
21	35.66116790892424	37.0	37.0	37.0	33.0	37.0
22	35.56267596425074	37.0	37.0	37.0	33.0	37.0
23	35.61657473160031	37.0	37.0	37.0	33.0	37.0
24	35.5966332284553	37.0	37.0	37.0	33.0	37.0
25	35.63000739216644	37.0	37.0	37.0	33.0	37.0
26	35.52035380990123	37.0	37.0	37.0	33.0	37.0
27	35.52996362627128	37.0	37.0	37.0	33.0	37.0
28	35.553441093506905	37.0	37.0	37.0	33.0	37.0
29	35.55507564294502	37.0	37.0	37.0	33.0	37.0
30	35.565619487565414	37.0	37.0	37.0	33.0	37.0
31	35.55900256457471	37.0	37.0	37.0	33.0	37.0
32	35.54081036291001	37.0	37.0	37.0	33.0	37.0
33	35.54017388937369	37.0	37.0	37.0	33.0	37.0
34	35.52364826097283	37.0	37.0	37.0	33.0	37.0
35	35.45695464091951	37.0	37.0	37.0	33.0	37.0
36	35.47797161087849	37.0	37.0	37.0	33.0	37.0
37	35.48385865750785	37.0	37.0	37.0	33.0	37.0
38	35.46054798103122	37.0	37.0	37.0	33.0	37.0
39	35.37033552963405	37.0	37.0	37.0	33.0	37.0
40	35.163788525329515	37.0	37.0	37.0	33.0	37.0
41	35.32835095978074	37.0	37.0	37.0	33.0	37.0
42	35.37237838285017	37.0	37.0	37.0	33.0	37.0
43	35.04169635542177	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	31.0
16	42.0
17	39.0
18	18.0
19	39.0
20	37.0
21	127.0
22	304.0
23	752.0
24	1548.0
25	2637.0
26	4348.0
27	6677.0
28	9663.0
29	13085.0
30	17633.0
31	22292.0
32	28673.0
33	38220.0
34	55564.0
35	115356.0
36	432351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.48665540495462	21.5460302465034	14.280891650054306	23.686422698487675
2	15.30778899501229	22.512215755188528	39.887676431264865	22.292318818534323
3	17.34583863727947	28.04940208848717	31.275802530415962	23.3289567438174
4	12.131692645995287	18.362595104090776	40.63650022283245	28.86921202708148
5	12.843689037977587	38.23031001731955	35.89150327843916	13.0344976662637
6	27.752514537482554	41.22493802055396	18.006596908099624	13.015950533863862
7	24.774165312325707	33.37189535681214	24.38294090803558	17.470998422826582
8	23.686956428916446	36.51690190835315	21.28183368426109	18.51430797846931
9	25.458407722011845	15.788413246121783	21.032448141417216	37.72073089044916
10	14.161069168794919	28.916847467849415	34.79361978645446	22.128463576901215
11	32.40410865684069	24.711051689123376	25.074388678510147	17.81045097552579
12	21.83864795407783	26.70266678408736	31.4352544960117	20.023430765823104
13	27.28176429930535	23.061424366395265	27.285900710128335	22.37091062417105
14	21.378572324476078	22.119390159612085	28.949271591397334	27.552765924514507
15	22.922654454914458	29.639118170585583	25.724739205969243	21.71348816853072
16	21.839048251899413	29.582142447314137	25.955444183806087	22.623365116980366
17	21.42981044563822	28.370307508786535	27.44962251915425	22.75025952642099
18	21.90122784685139	27.82163262800857	29.101117898383063	21.176021626756974
19	23.811849349249176	27.02770861520972	29.079635248624975	20.080806786916135
20	23.416888831957642	26.87786379733188	28.973022595477705	20.732224775232773
21	21.86706909940996	27.898222944537405	28.51841770277086	21.716290253281777
22	21.470507390832115	27.963204624240433	28.72243615916909	21.843851825758364
23	21.650507977935586	27.523677616146415	29.1332751567166	21.692539249201406
24	22.03372642579412	28.003100973791167	28.1510777351683	21.812094865246408
25	22.090435283851185	27.597065550102613	28.92498685688819	21.387512309158012
26	21.977951595987413	27.690068077316187	28.65571985557255	21.676260471123847
27	21.824504097715366	27.74464201365816	28.3015897160821	22.12926417254437
28	21.28410203858337	27.851121234198246	29.055483946723026	21.809292780495355
29	22.45137048630848	27.699541792426903	28.35442902853056	21.494658692734063
30	21.854259569119424	27.964005219883592	28.747921787142968	21.433813423854016
31	22.096039453353296	27.65350754294528	28.653051203428685	21.597401800272735
32	21.16427955732398	27.538755500759233	28.95340800222032	22.34355693969647
33	21.248875830284398	27.436012393220555	29.443906266262097	21.871205510232947
34	21.721360692355113	27.439481641007575	28.809300786451786	22.029856880185523
35	21.810226808745707	28.066481462207882	28.475986133683463	21.64730559536295
36	22.30726327053995	27.80655474339575	28.246482049311357	21.639699936752944
37	22.346492457054715	27.15033318122016	28.253954275314168	22.249220086410958
38	22.206655084716363	27.72382652693604	28.437690975419045	21.631827412928555
39	21.757254063690052	27.423603160751597	28.692814120372223	22.126328655186125
40	21.80422234142202	27.781069115421875	28.868544864045514	21.54616367911059
41	21.599803587202214	27.511401816284646	28.852399518575155	22.036395077937986
42	21.612879982707135	27.670720349273193	28.697884559445562	22.018515108574114
43	21.78060476994884	27.312053501138177	28.708826033235397	22.198515695677585
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	78.5
2	86.0
3	288.0
4	490.0
5	490.0
6	579.5
7	669.0
8	596.5
9	524.0
10	809.0
11	1094.0
12	1094.0
13	1703.5
14	2313.0
15	4360.5
16	6408.0
17	7345.5
18	8283.0
19	8283.0
20	8329.5
21	8376.0
22	9745.5
23	11115.0
24	13610.0
25	16105.0
26	16105.0
27	19275.5
28	22446.0
29	27168.0
30	31890.0
31	34423.0
32	36956.0
33	36956.0
34	40671.0
35	44386.0
36	46623.5
37	48861.0
38	51321.5
39	53782.0
40	53782.0
41	55946.5
42	58111.0
43	56279.0
44	54447.0
45	53820.5
46	53194.0
47	53194.0
48	51171.0
49	49148.0
50	49552.0
51	49956.0
52	48686.5
53	47417.0
54	47417.0
55	43255.5
56	39094.0
57	34600.0
58	30106.0
59	26806.5
60	23507.0
61	23507.0
62	20449.5
63	17392.0
64	14410.5
65	11429.0
66	9693.0
67	7957.0
68	7957.0
69	6513.0
70	5069.0
71	4340.5
72	3612.0
73	2957.5
74	2303.0
75	2303.0
76	1723.5
77	1144.0
78	921.5
79	699.0
80	546.5
81	394.0
82	394.0
83	311.5
84	229.0
85	202.0
86	175.0
87	152.5
88	130.0
89	130.0
90	92.5
91	55.0
92	35.5
93	16.0
94	9.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	749442.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.906698504964496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.97306185176512	53.58148754764051
2	7.191173560068198	8.759812798398919
3	1.7222690081362975	3.146931576690051
4	0.746205565381094	1.8179566957357143
5	0.42989409836416137	1.3091715119064766
6	0.29465320893219077	1.0767812495971953
7	0.18889029083463613	0.805327879606617
8	0.16555032580214338	0.8066499024823833
9	0.12346484656351794	0.67678525670453
>10	0.9499154489324315	11.914281830196261
>50	0.13684925800796505	5.933000878144597
>100	0.07518917646305374	8.156146646193191
>500	0.002217969807033509	0.8193161091720821
>1k	6.653909421100527E-4	1.196350117531403
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4140	0.5524109937793719	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3059	0.408170345403647	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1657	0.22109783011894182	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	851	0.11355114872131533	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	832	0.1110159291846467	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.6686521438617E-4	0.0	0.0
2	1.33432607193085E-4	0.0	2.6686521438617E-4	0.0	0.0
3	1.33432607193085E-4	0.0	4.0029782157925497E-4	0.0	0.0
4	1.33432607193085E-4	0.0	4.0029782157925497E-4	1.33432607193085E-4	0.0
5	1.33432607193085E-4	0.0	8.005956431585099E-4	1.33432607193085E-4	0.0
6	1.33432607193085E-4	0.0	0.001200893464737765	1.33432607193085E-4	0.0
7	1.33432607193085E-4	0.0	0.0032023825726340397	4.0029782157925497E-4	0.0
8	2.6686521438617E-4	0.0	0.004136410822985635	4.0029782157925497E-4	0.0
9	2.6686521438617E-4	0.0	0.004670141251757975	0.00133432607193085	0.0
10	5.3373042877234E-4	0.0	0.004937006466144145	0.0014677586791239348	0.0
11	5.3373042877234E-4	0.0	0.00747222600281276	0.0016011912863170199	0.0
12	5.3373042877234E-4	0.0	0.008139389038778184	0.0016011912863170199	0.0
13	5.3373042877234E-4	0.0	0.00907341728912978	0.0016011912863170199	0.0
14	5.3373042877234E-4	0.0	0.010541175968253715	0.001734623893510105	0.0
15	6.67163035965425E-4	0.0	0.011208339004219138	0.00186805650070319	0.0
16	6.67163035965425E-4	0.0	0.011475204218605308	0.00186805650070319	0.0
17	6.67163035965425E-4	0.0	0.011475204218605308	0.00186805650070319	0.0
18	6.67163035965425E-4	0.0	0.011608636825798394	0.0020014891078962748	0.0
19	6.67163035965425E-4	0.0	0.012142367254570734	0.0020014891078962748	0.0
20	6.67163035965425E-4	0.0	0.012409232468956904	0.00240178692947553	0.0
21	6.67163035965425E-4	0.0	0.012542665076149989	0.00240178692947553	0.0
22	6.67163035965425E-4	0.0	0.012676097683343074	0.0026686521438617	0.0
23	6.67163035965425E-4	0.0	0.012809530290536159	0.0030689499654409547	0.0
24	6.67163035965425E-4	0.0	0.012942962897729244	0.0033358151798271248	0.0
25	6.67163035965425E-4	0.0	0.012942962897729244	0.00346924778702021	0.0
26	6.67163035965425E-4	0.0	0.013076395504922329	0.00373611300140638	0.0
27	6.67163035965425E-4	0.0	0.013076395504922329	0.004403276037371805	0.0
28	6.67163035965425E-4	0.0	0.013076395504922329	0.010941473789832968	0.0
29	6.67163035965425E-4	0.0	0.013076395504922329	0.026686521438616998	0.0
30	6.67163035965425E-4	0.0	0.013076395504922329	0.04896976683986219	0.0
31	6.67163035965425E-4	0.0	0.013076395504922329	0.1012753488595515	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	675	0.0	27.955555	1
TATTGCG	35	8.8665023E-4	26.428572	9
CCGGTTC	60	1.3363697E-6	24.666668	21
GTATCAA	1935	0.0	23.997414	1
TTCGCCG	85	1.9736035E-9	23.941175	24
CCCGGTT	70	1.9210711E-7	23.785715	20
CGGTTCG	65	2.6807775E-6	22.76923	22
GTCGCCA	110	7.4578566E-11	21.863636	12
AGACCCG	45	0.0038245847	20.555555	5
GCCGGCA	110	1.7516868E-9	20.181818	15
TTGGCCG	140	3.6379788E-12	19.82143	31
ATCGTAC	75	9.258714E-6	19.733334	25
GGTTCGC	75	9.258714E-6	19.733334	23
CGAACCG	180	0.0	19.527777	31
TCGCCAT	130	6.9485395E-10	18.5	13
CCGCTGA	100	2.8716022E-7	18.5	26
GTTCGCC	80	1.6154469E-5	18.5	24
CTACGGT	50	0.007032654	18.5	27
CGGGATT	90	2.1501837E-6	18.5	17
GGCCGCA	155	2.0008883E-11	17.903227	33
>>END_MODULE