FastQCFastQC Report
Fri 10 Feb 2017
ERR1633474.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633474.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714295
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53380.7473102849662955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40060.5608327091747807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21440.3001560979707264No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13300.1861975794314674No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12420.17387773958938535No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12270.17177776688903046No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11310.15833794160675912No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG11310.15833794160675912No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10850.15189802532567076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.14699808902484268No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10090.14125816364387264No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10060.14083816910380165No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9600.1343982528227133No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA9550.133698261922595No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9400.1315982892222401No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8950.12529837112117542No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC8890.12445838204103346No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT8790.12305840024079687No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG8760.1226384057007259No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT8510.1191384512001344No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8440.11815846393996877No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8380.11731847485982683No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT8360.11703847849977951No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT8010.11213854219895142No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7760.10863858769835993No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA7600.10639861681798136No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC7490.1048586368377211No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7490.1048586368377211No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT7430.10401864775757914No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG7370.1031786586774372No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG7290.10205867323724793No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7280.10191867505722427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGC250.005494968529.59999817
GGTATCA8800.027.9602281
TTAGACT358.866094E-426.428574
GTATCAA24450.024.4396721
TTCGCCG1054.0017767E-1122.9047624
TCTAGAC652.6804846E-622.769233
GTGTATC502.700715E-422.1999991
GCCGCTC1107.4578566E-1121.86363627
ATTAGAC603.7237445E-521.5833323
TAGGACC1900.021.4210534
TGCTCGC1450.020.41379410
GCTCGGT1202.382876E-1020.04166626
CGCTCTC1303.274181E-1119.92307729
AGCTTCG1403.6379788E-1219.82142821
TGCGTTA759.257721E-619.73333437
CTTGCTA1600.019.6562516
CCGCTCT1254.110916E-1019.2428
CTAGACA801.6152742E-518.54
GCCGGCA1501.2732926E-1118.515
TCTAAGC701.2183774E-418.53
Produced by FastQC (version 0.11.5)