##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633474.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	714295
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.908071595069263	33.0	33.0	33.0	33.0	33.0
2	32.01513380326056	33.0	33.0	33.0	33.0	33.0
3	31.7455729075522	33.0	33.0	33.0	27.0	33.0
4	32.02280150358045	33.0	33.0	33.0	33.0	33.0
5	32.118673657242454	33.0	33.0	33.0	33.0	33.0
6	35.2911010156868	37.0	37.0	37.0	33.0	37.0
7	35.436822321309826	37.0	37.0	37.0	33.0	37.0
8	35.566106440616274	37.0	37.0	37.0	33.0	37.0
9	35.680622151912026	37.0	37.0	37.0	33.0	37.0
10	35.69227840038079	37.0	37.0	37.0	33.0	37.0
11	35.703804450542144	37.0	37.0	37.0	33.0	37.0
12	35.69550255846674	37.0	37.0	37.0	33.0	37.0
13	35.705165232851975	37.0	37.0	37.0	33.0	37.0
14	35.670564682659126	37.0	37.0	37.0	33.0	37.0
15	35.68614648009576	37.0	37.0	37.0	33.0	37.0
16	35.683896709342775	37.0	37.0	37.0	33.0	37.0
17	35.66101120685431	37.0	37.0	37.0	33.0	37.0
18	35.65686166079841	37.0	37.0	37.0	33.0	37.0
19	35.64487501662478	37.0	37.0	37.0	33.0	37.0
20	35.64043147439083	37.0	37.0	37.0	33.0	37.0
21	35.63578213483225	37.0	37.0	37.0	33.0	37.0
22	35.54524671179275	37.0	37.0	37.0	33.0	37.0
23	35.59952400618792	37.0	37.0	37.0	33.0	37.0
24	35.59333048670367	37.0	37.0	37.0	33.0	37.0
25	35.598440420274535	37.0	37.0	37.0	33.0	37.0
26	35.50683121119425	37.0	37.0	37.0	33.0	37.0
27	35.506540014979805	37.0	37.0	37.0	33.0	37.0
28	35.53423725491569	37.0	37.0	37.0	33.0	37.0
29	35.54211075256022	37.0	37.0	37.0	33.0	37.0
30	35.54445152213021	37.0	37.0	37.0	33.0	37.0
31	35.52385498988513	37.0	37.0	37.0	33.0	37.0
32	35.52803953548604	37.0	37.0	37.0	33.0	37.0
33	35.5201282383329	37.0	37.0	37.0	33.0	37.0
34	35.49380018059765	37.0	37.0	37.0	33.0	37.0
35	35.425159072932054	37.0	37.0	37.0	33.0	37.0
36	35.44432342379549	37.0	37.0	37.0	33.0	37.0
37	35.435412539636985	37.0	37.0	37.0	33.0	37.0
38	35.40910968157414	37.0	37.0	37.0	33.0	37.0
39	35.30667441323263	37.0	37.0	37.0	33.0	37.0
40	35.099659104431645	37.0	37.0	37.0	33.0	37.0
41	35.25331270693481	37.0	37.0	37.0	33.0	37.0
42	35.27282425328471	37.0	37.0	37.0	33.0	37.0
43	34.931377092097804	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	22.0
16	53.0
17	28.0
18	23.0
19	23.0
20	50.0
21	95.0
22	275.0
23	738.0
24	1356.0
25	2576.0
26	4356.0
27	6625.0
28	9229.0
29	12867.0
30	16956.0
31	21741.0
32	28492.0
33	37242.0
34	53736.0
35	110522.0
36	407288.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92259500626492	20.718330661701398	13.590743320336834	23.768331011696848
2	16.316787881757538	22.056013271827464	38.76605604127146	22.861142805143533
3	17.491792606696112	27.650900538293	30.823959288529252	24.033347566481638
4	11.771466970929378	17.863907769199	41.19404447742179	29.17058078244983
5	13.416725582567427	37.33443465234952	35.30852098922714	13.940318775855914
6	28.018955753575202	41.64875856613864	16.984439202290368	13.347846477995787
7	25.993462084992895	33.17116877480593	23.02480067759119	17.810568462609986
8	24.462581986434177	34.863886769471996	20.56769261999594	20.105838624097885
9	26.383917009078882	14.350233446965188	20.658831435191345	38.607018108764585
10	15.457759049132362	27.42284350303446	33.35498638517699	23.764411062656183
11	33.5236841921055	23.67383224018088	24.170685781084845	18.631797786628773
12	23.25243771830966	26.69485296691143	29.551375832114186	20.501333482664723
13	28.072015063804173	22.82166331837686	25.898823315296905	23.20749830252207
14	22.002253970698384	21.55733975458319	28.670227287045268	27.770178987673162
15	24.393002890962418	28.076775001924975	25.997942026753652	21.532280080358955
16	22.18327161746897	28.736166429836413	25.52628815825394	23.55427379444067
17	22.055173282747322	27.09006782911822	26.465255951672628	24.389502936461827
18	22.830623201898376	26.530495103563656	28.846204999335008	21.79267669520296
19	25.494228575028526	25.442149252059725	28.363491274613427	20.700130898298323
20	24.92667595321261	25.329450717140677	28.651327532742076	21.09254579690464
21	23.16703882849523	27.017548771865968	28.44651019536746	21.368902204271343
22	22.196711442751244	26.872230661001407	28.56690863018781	22.364149266059542
23	22.299190110528563	26.393576883500515	29.502796463645975	21.80443654232495
24	22.78526379157071	26.848570968577405	27.42648345571508	22.939681784136805
25	22.936881820536335	26.919550045849405	28.562148692067	21.581419441547258
26	22.99722103612653	27.249385757985145	28.18751356232369	21.565879643564635
27	22.829503216458185	26.994309073982038	27.720059639224694	22.456128070335087
28	21.649598555218784	26.855850873938643	28.897864327763738	22.59668624307884
29	23.790030729600513	26.565214652209523	27.54996185049594	22.09479276769402
30	23.434575350520443	25.969242399848802	28.991103115659495	21.60507913397126
31	22.98728116534485	26.702552866812727	28.065995142063155	22.244170825779264
32	21.12894532371079	26.174759728123536	29.233019970740383	23.463274977425293
33	21.39326188759546	26.36893720381635	29.742473347846477	22.49532756074171
34	22.189011542849943	26.396936839821084	29.12326139760183	22.290790219727143
35	22.419028552628816	27.351304433042372	27.977096297748126	22.252570716580685
36	23.531454091096816	26.704512841333063	28.047095387759956	21.716937679810165
37	22.68572508557389	26.142000153997998	28.137674210235268	23.034600550192845
38	22.749704253844698	26.3899369308199	28.211453251107738	22.648905564227665
39	22.247250785739787	26.468615907993197	28.522389208940286	22.761744097326737
40	22.283090319825842	27.658180443654235	28.480949747653277	21.577779488866643
41	22.50554742788344	25.978482279730365	28.56116870480684	22.95480158757936
42	21.69047802378569	27.466522935201844	28.444270224487084	22.398728816525384
43	22.53858699836902	26.035321540819968	28.091334812647435	23.334756648163573
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	58.0
2	92.0
3	323.5
4	555.0
5	555.0
6	675.0
7	795.0
8	795.5
9	796.0
10	1063.0
11	1330.0
12	1330.0
13	2107.5
14	2885.0
15	5015.5
16	7146.0
17	7865.5
18	8585.0
19	8585.0
20	7790.5
21	6996.0
22	7174.0
23	7352.0
24	9205.5
25	11059.0
26	11059.0
27	13036.5
28	15014.0
29	16931.0
30	18848.0
31	23029.5
32	27211.0
33	27211.0
34	33003.0
35	38795.0
36	42183.5
37	45572.0
38	47468.0
39	49364.0
40	49364.0
41	51133.5
42	52903.0
43	54859.5
44	56816.0
45	54412.0
46	52008.0
47	52008.0
48	50688.5
49	49369.0
50	49922.0
51	50475.0
52	49111.0
53	47747.0
54	47747.0
55	46371.0
56	44995.0
57	38618.5
58	32242.0
59	29844.0
60	27446.0
61	27446.0
62	23844.5
63	20243.0
64	16511.5
65	12780.0
66	10829.0
67	8878.0
68	8878.0
69	7421.0
70	5964.0
71	5011.5
72	4059.0
73	3318.5
74	2578.0
75	2578.0
76	1883.0
77	1188.0
78	960.0
79	732.0
80	596.5
81	461.0
82	461.0
83	423.5
84	386.0
85	352.0
86	318.0
87	245.0
88	172.0
89	172.0
90	135.0
91	98.0
92	56.0
93	14.0
94	8.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	714295.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.599453238081594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.36473711603338	42.45878456127956
2	7.165088713534705	6.964387877602722
3	1.7494553593727025	2.5506772178983463
4	0.8444974615603316	1.6416845957111976
5	0.46017359988562884	1.1182092674520645
6	0.33958956961402653	0.9902320445158281
7	0.23280717821483887	0.7920011099799251
8	0.17969567195569064	0.698648912503699
9	0.14387149038410021	0.6292868192293665
>10	1.1425534704925535	11.325861684273177
>50	0.18583859761352933	6.306370268996285
>100	0.1738395605726564	16.635452808356728
>500	0.014340300451279615	4.802137406216992
>1k	0.003219251121715832	2.327037588576732
>5k	2.9265919288325745E-4	0.7592278374074076
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5338	0.7473102849662955	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4006	0.5608327091747807	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2144	0.3001560979707264	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1330	0.1861975794314674	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1242	0.17387773958938535	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1227	0.17177776688903046	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1131	0.15833794160675912	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1131	0.15833794160675912	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1085	0.15189802532567076	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1050	0.14699808902484268	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1009	0.14125816364387264	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1006	0.14083816910380165	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	960	0.1343982528227133	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	955	0.133698261922595	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	940	0.1315982892222401	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	895	0.12529837112117542	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	889	0.12445838204103346	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	879	0.12305840024079687	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	876	0.1226384057007259	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	851	0.1191384512001344	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	844	0.11815846393996877	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	838	0.11731847485982683	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	836	0.11703847849977951	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	801	0.11213854219895142	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	776	0.10863858769835993	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	760	0.10639861681798136	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	749	0.1048586368377211	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	749	0.1048586368377211	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	743	0.10401864775757914	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	737	0.1031786586774372	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	729	0.10205867323724793	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	728	0.10191867505722427	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	1.399981800236597E-4	0.0	0.0
4	0.0	0.0	5.599927200946388E-4	1.399981800236597E-4	0.0
5	0.0	0.0	6.999909001182984E-4	1.399981800236597E-4	0.0
6	0.0	0.0	0.0016799781602839163	1.399981800236597E-4	0.0
7	0.0	0.0	0.00489993630082809	1.399981800236597E-4	0.0
8	1.399981800236597E-4	0.0	0.005459929020922728	1.399981800236597E-4	0.0
9	1.399981800236597E-4	0.0	0.008119894441372262	4.1999454007097907E-4	0.0
10	2.799963600473194E-4	0.0	0.010219867141727158	5.599927200946388E-4	0.0
11	4.1999454007097907E-4	0.0	0.014839807082507928	5.599927200946388E-4	0.0
12	4.1999454007097907E-4	0.0	0.015679796162649886	6.999909001182984E-4	0.0
13	4.1999454007097907E-4	0.0	0.017079777962886483	8.399890801419581E-4	0.0
14	4.1999454007097907E-4	0.0	0.0180597652230521	9.799872601656179E-4	0.0
15	4.1999454007097907E-4	0.0	0.019459747023288697	9.799872601656179E-4	0.0
16	4.1999454007097907E-4	0.0	0.020859728823525294	9.799872601656179E-4	0.0
17	4.1999454007097907E-4	0.0	0.021139725183572614	0.0012599836202129373	0.0
18	4.1999454007097907E-4	0.0	0.021979714263714572	0.0012599836202129373	0.0
19	4.1999454007097907E-4	0.0	0.02239970880378555	0.0013999818002365968	0.0
20	4.1999454007097907E-4	0.0	0.023239697883927508	0.0015399799802602566	0.0
21	4.1999454007097907E-4	0.0	0.023799690604022147	0.001819976340307576	0.0
22	4.1999454007097907E-4	0.0	0.024079686964069466	0.002239970880378555	0.0
23	4.1999454007097907E-4	0.0	0.024219685144093128	0.002659965420449534	0.0
24	4.1999454007097907E-4	0.0	0.024219685144093128	0.0034999545005914923	0.0
25	4.1999454007097907E-4	0.0	0.024219685144093128	0.004339943580733451	0.0
26	4.1999454007097907E-4	0.0	0.024219685144093128	0.00475993812080443	0.0
27	4.1999454007097907E-4	0.0	0.024359683324116786	0.006719912641135665	0.0
28	4.1999454007097907E-4	0.0	0.024359683324116786	0.016659783422815502	0.0
29	4.1999454007097907E-4	0.0	0.024359683324116786	0.043399435807334505	0.0
30	4.1999454007097907E-4	0.0	0.024359683324116786	0.07923896989339138	0.0
31	4.1999454007097907E-4	0.0	0.024359683324116786	0.15315800894588372	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTGCGC	25	0.0054949685	29.599998	17
GGTATCA	880	0.0	27.960228	1
TTAGACT	35	8.866094E-4	26.42857	4
GTATCAA	2445	0.0	24.439672	1
TTCGCCG	105	4.0017767E-11	22.90476	24
TCTAGAC	65	2.6804846E-6	22.76923	3
GTGTATC	50	2.700715E-4	22.199999	1
GCCGCTC	110	7.4578566E-11	21.863636	27
ATTAGAC	60	3.7237445E-5	21.583332	3
TAGGACC	190	0.0	21.421053	4
TGCTCGC	145	0.0	20.413794	10
GCTCGGT	120	2.382876E-10	20.041666	26
CGCTCTC	130	3.274181E-11	19.923077	29
AGCTTCG	140	3.6379788E-12	19.821428	21
TGCGTTA	75	9.257721E-6	19.733334	37
CTTGCTA	160	0.0	19.65625	16
CCGCTCT	125	4.110916E-10	19.24	28
CTAGACA	80	1.6152742E-5	18.5	4
GCCGGCA	150	1.2732926E-11	18.5	15
TCTAAGC	70	1.2183774E-4	18.5	3
>>END_MODULE