FastQCFastQC Report
Fri 10 Feb 2017
ERR1633473.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633473.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301410
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12690.4210212003583159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.30556384990544444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6550.21731196708801964No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT4240.14067217411499286No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG3280.10882187054178692No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA3190.10583590458179888No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG3020.100195746657377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGGC250.005489991529.625
GCGGGCT250.005489991529.626
CTAGAGC358.854159E-426.428573
GCCTAAA509.062063E-625.90000211
CGGAGTC509.062063E-625.90000227
GGCCTAA509.062063E-625.90000210
AGGTCCT509.062063E-625.90000218
CCGTCTA451.3200507E-424.6666662
GCCGTAT601.3319441E-624.6666668
GCAATTA701.9131403E-723.78571317
CTTAGGC952.7830538E-1023.3684223
GTTGGCG400.001927900723.12500235
GGCCGTA652.6719554E-622.769237
CCGTATG652.6719554E-622.769239
GGTATCA2400.022.3541661
ATGTGAG502.695698E-422.214
CCCGTCT502.695698E-422.21
TTAACGG502.695698E-422.235
GTATCAA5350.021.7850461
GAAAGCG603.714558E-521.58333234
Produced by FastQC (version 0.11.5)