##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633473.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301410
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.842115390995655	33.0	33.0	33.0	33.0	33.0
2	31.952035433462726	33.0	33.0	33.0	33.0	33.0
3	31.68085000497661	33.0	33.0	33.0	27.0	33.0
4	31.963040376895258	33.0	33.0	33.0	33.0	33.0
5	32.05467635446734	33.0	33.0	33.0	33.0	33.0
6	35.23245081450516	37.0	37.0	37.0	33.0	37.0
7	35.36001791579576	37.0	37.0	37.0	33.0	37.0
8	35.4858133439501	37.0	37.0	37.0	33.0	37.0
9	35.602129989051456	37.0	37.0	37.0	33.0	37.0
10	35.618914435486545	37.0	37.0	37.0	33.0	37.0
11	35.63893699611825	37.0	37.0	37.0	33.0	37.0
12	35.63548654656448	37.0	37.0	37.0	33.0	37.0
13	35.63458080355662	37.0	37.0	37.0	33.0	37.0
14	35.61077933711556	37.0	37.0	37.0	33.0	37.0
15	35.615035997478515	37.0	37.0	37.0	33.0	37.0
16	35.61230881523506	37.0	37.0	37.0	33.0	37.0
17	35.57011048074052	37.0	37.0	37.0	33.0	37.0
18	35.58101920971434	37.0	37.0	37.0	33.0	37.0
19	35.5899406124548	37.0	37.0	37.0	33.0	37.0
20	35.58747884940778	37.0	37.0	37.0	33.0	37.0
21	35.59254172057994	37.0	37.0	37.0	33.0	37.0
22	35.50366942039083	37.0	37.0	37.0	33.0	37.0
23	35.54230450217312	37.0	37.0	37.0	33.0	37.0
24	35.54098735941077	37.0	37.0	37.0	33.0	37.0
25	35.53689990378554	37.0	37.0	37.0	33.0	37.0
26	35.43461066321622	37.0	37.0	37.0	33.0	37.0
27	35.44635214491888	37.0	37.0	37.0	33.0	37.0
28	35.467101290600844	37.0	37.0	37.0	33.0	37.0
29	35.47610895458014	37.0	37.0	37.0	33.0	37.0
30	35.46144454397664	37.0	37.0	37.0	33.0	37.0
31	35.47532265021068	37.0	37.0	37.0	33.0	37.0
32	35.459483759662916	37.0	37.0	37.0	33.0	37.0
33	35.464141866560496	37.0	37.0	37.0	33.0	37.0
34	35.432527122524135	37.0	37.0	37.0	33.0	37.0
35	35.398075710825786	37.0	37.0	37.0	33.0	37.0
36	35.41591851630669	37.0	37.0	37.0	33.0	37.0
37	35.41747121860588	37.0	37.0	37.0	33.0	37.0
38	35.410467469559734	37.0	37.0	37.0	33.0	37.0
39	35.317069772071264	37.0	37.0	37.0	33.0	37.0
40	35.11036793736107	37.0	37.0	37.0	33.0	37.0
41	35.27607909492054	37.0	37.0	37.0	33.0	37.0
42	35.323655485883016	37.0	37.0	37.0	33.0	37.0
43	35.00647954613317	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	6.0
16	15.0
17	11.0
18	11.0
19	9.0
20	31.0
21	55.0
22	153.0
23	312.0
24	661.0
25	1191.0
26	2088.0
27	3041.0
28	4177.0
29	5623.0
30	7345.0
31	9210.0
32	12002.0
33	15988.0
34	23088.0
35	47061.0
36	169329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.577253574864805	20.447563120002656	13.48429050131051	24.490892803822035
2	16.330911383165787	21.537772469393847	38.418433363192996	23.712882784247373
3	18.387246607610898	25.857469891509904	30.152284263959388	25.60299923691981
4	12.97136790418367	17.252579542815436	39.26346172986961	30.51259082313128
5	13.86184930825122	37.056501111442884	35.20387512026807	13.877774460037823
6	30.515245015095715	39.636707474868125	16.543246740320495	13.30480076971567
7	27.613549649978435	31.54009488736273	22.807803324375435	18.0385521382834
8	24.69493381108789	36.02435221127368	20.198732623336983	19.08198135430145
9	25.425500149298298	15.234730101854616	20.48737599946916	38.852393749377924
10	14.60966789423045	28.661291927938688	33.55429481437245	23.174745363458413
11	33.781228227331546	23.74904614976278	24.089114495205866	18.38061112769981
12	23.38409475465313	25.763577850768055	30.077966888955242	20.77436050562357
13	27.963571215288148	22.30450217311967	26.56514382402707	23.16678278756511
14	22.58518297335855	20.694402972695	27.3398361036462	29.380577950300257
15	23.479313891377192	29.42238147374009	24.517766497461928	22.58053813742079
16	23.45874390365283	28.372980325802068	24.860489034869445	23.30778673567566
17	22.20331110447563	27.914800437941672	26.458976145449718	23.422912312132976
18	23.36452008891543	26.966258584652138	27.828870973093128	21.840350353339304
19	24.713844928834476	25.913871470754124	27.54819017285425	21.82409342755715
20	23.570219966159055	27.069108523273943	27.607577718058458	21.753093792508544
21	22.675093726153744	26.96791745462991	28.276765867091335	22.080222952125013
22	22.654191964433828	26.56016721409376	27.82323081516871	22.962410006303706
23	22.651537772469393	26.50442918284065	27.81659533525762	23.027437709432334
24	22.650210676487177	27.208453601406724	26.914833615341227	23.226502106764872
25	23.414949736239674	27.219070369264458	27.27912146245977	22.086858432036095
26	23.67771474071862	26.443714541654224	27.48415779171229	22.394412925914867
27	22.981321124050297	26.65737699479115	27.30798580007299	23.053316081085566
28	22.307819913075214	26.442055671676457	28.14903287880296	23.101091536445374
29	23.374805082777613	26.417836170000996	26.9556418167944	23.251716930426994
30	22.734813045353505	26.686904880395474	28.290036826913507	22.288245247337514
31	23.165455691582892	26.4417238976809	28.154341262731826	22.238479148004377
32	22.822069606184268	26.500116120898443	27.88162303838625	22.796191234531037
33	22.882452473375135	26.58140074980923	28.129458213065263	22.406688563750375
34	23.39902458445307	25.92681065658074	27.661656879333798	23.012507879632395
35	22.5500149298298	27.230682459108856	27.78806277163996	22.431239839421384
36	23.368833150857636	26.201187750904083	28.046846488172257	22.383132610066024
37	23.224511462791547	26.21014564878405	27.535582761023193	23.029760127401214
38	23.275604658106896	26.41584552602767	27.990113134932482	22.318436680932948
39	23.202614379084967	26.522344978600575	27.812614047310973	22.462426595003485
40	22.94383066255267	27.44135894628579	27.526956637138785	22.08785375402276
41	23.678710062705285	25.84021764374108	27.48283069573007	22.99824159782356
42	22.791878172588834	26.688563750373245	27.425765568494743	23.09379250854318
43	23.08748880262765	26.21279984074848	27.59696095020072	23.102750406423144
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	34.5
2	44.0
3	106.5
4	169.0
5	169.0
6	189.0
7	209.0
8	172.5
9	136.0
10	200.0
11	264.0
12	264.0
13	431.0
14	598.0
15	1129.5
16	1661.0
17	1960.0
18	2259.0
19	2259.0
20	2284.0
21	2309.0
22	2453.0
23	2597.0
24	3284.0
25	3971.0
26	3971.0
27	5009.0
28	6047.0
29	7288.0
30	8529.0
31	10028.5
32	11528.0
33	11528.0
34	13464.0
35	15400.0
36	17064.0
37	18728.0
38	19702.5
39	20677.0
40	20677.0
41	21554.0
42	22431.0
43	23271.0
44	24111.0
45	23935.5
46	23760.0
47	23760.0
48	22750.0
49	21740.0
50	22127.5
51	22515.0
52	21596.5
53	20678.0
54	20678.0
55	19709.5
56	18741.0
57	16384.5
58	14028.0
59	13152.0
60	12276.0
61	12276.0
62	11252.0
63	10228.0
64	8327.5
65	6427.0
66	5216.5
67	4006.0
68	4006.0
69	3115.0
70	2224.0
71	1809.0
72	1394.0
73	1067.0
74	740.0
75	740.0
76	568.5
77	397.0
78	306.5
79	216.0
80	172.0
81	128.0
82	128.0
83	110.5
84	93.0
85	72.5
86	52.0
87	48.5
88	45.0
89	45.0
90	32.5
91	20.0
92	13.5
93	7.0
94	4.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	301410.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.332688862542824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.16627942668184	50.26310768239557
2	6.778292546667602	7.907920602881085
3	2.148007343787254	3.7589713217879686
4	1.1250525547811114	2.6250936252822195
5	0.7503330203892733	2.1884471310829743
6	0.4891219863114986	1.7119080386002548
7	0.35616166656425713	1.4543107376320272
8	0.28656449658129607	1.3372862094502367
9	0.20730181446957144	1.0883225019684684
>10	1.530112141551433	17.15136662576098
>50	0.11330841496936192	4.653550113489871
>100	0.0477390783881068	4.905184041760773
>500	0.0011503392382676337	0.5287667612732543
>1k	5.751696191338169E-4	0.42576460663436533
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1269	0.4210212003583159	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	921	0.30556384990544444	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	655	0.21731196708801964	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	424	0.14067217411499286	No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG	328	0.10882187054178692	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	319	0.10583590458179888	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	302	0.100195746657377	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	3.3177399555422846E-4	0.0	0.0
7	0.0	0.0	9.953219866626853E-4	0.0	0.0
8	0.0	0.0	9.953219866626853E-4	0.0	0.0
9	0.0	0.0	9.953219866626853E-4	6.635479911084569E-4	0.0
10	0.0	0.0	9.953219866626853E-4	6.635479911084569E-4	0.0
11	0.0	0.0	0.0013270959822169138	6.635479911084569E-4	0.0
12	0.0	0.0	0.0013270959822169138	6.635479911084569E-4	0.0
13	0.0	0.0	0.0013270959822169138	6.635479911084569E-4	0.0
14	0.0	0.0	0.0013270959822169138	9.953219866626853E-4	0.0
15	0.0	0.0	0.0013270959822169138	0.0013270959822169138	0.0
16	0.0	0.0	0.0013270959822169138	0.0013270959822169138	0.0
17	0.0	0.0	0.0016588699777711424	0.0016588699777711424	0.0
18	0.0	0.0	0.0016588699777711424	0.0016588699777711424	0.0
19	0.0	0.0	0.0016588699777711424	0.0016588699777711424	0.0
20	0.0	0.0	0.0019906439733253707	0.0016588699777711424	0.0
21	0.0	0.0	0.002322417968879599	0.0019906439733253707	0.0
22	0.0	0.0	0.002322417968879599	0.0019906439733253707	0.0
23	0.0	0.0	0.002322417968879599	0.0019906439733253707	0.0
24	0.0	0.0	0.002322417968879599	0.002322417968879599	0.0
25	0.0	0.0	0.002322417968879599	0.0026541919644338277	0.0
26	0.0	0.0	0.0026541919644338277	0.0033177399555422847	0.0
27	0.0	0.0	0.0026541919644338277	0.003981287946650741	0.0
28	0.0	0.0	0.0026541919644338277	0.008957897879964169	0.0
29	0.0	0.0	0.0026541919644338277	0.01692047377326565	0.0
30	0.0	0.0	0.0026541919644338277	0.02786901562655519	0.0
31	0.0	0.0	0.0026541919644338277	0.06071464118642381	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGGGC	25	0.0054899915	29.6	25
GCGGGCT	25	0.0054899915	29.6	26
CTAGAGC	35	8.854159E-4	26.42857	3
GCCTAAA	50	9.062063E-6	25.900002	11
CGGAGTC	50	9.062063E-6	25.900002	27
GGCCTAA	50	9.062063E-6	25.900002	10
AGGTCCT	50	9.062063E-6	25.900002	18
CCGTCTA	45	1.3200507E-4	24.666666	2
GCCGTAT	60	1.3319441E-6	24.666666	8
GCAATTA	70	1.9131403E-7	23.785713	17
CTTAGGC	95	2.7830538E-10	23.368422	3
GTTGGCG	40	0.0019279007	23.125002	35
GGCCGTA	65	2.6719554E-6	22.76923	7
CCGTATG	65	2.6719554E-6	22.76923	9
GGTATCA	240	0.0	22.354166	1
ATGTGAG	50	2.695698E-4	22.2	14
CCCGTCT	50	2.695698E-4	22.2	1
TTAACGG	50	2.695698E-4	22.2	35
GTATCAA	535	0.0	21.785046	1
GAAAGCG	60	3.714558E-5	21.583332	34
>>END_MODULE