FastQCFastQC Report
Fri 10 Feb 2017
ERR1633472.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633472.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935836
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52030.5559734825332644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37010.39547527558247386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20790.22215430908834455No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC18180.19426480708158267No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG17250.1843271684354951No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC14890.1591090746669288No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA14160.15130856261139772No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12790.13666924546608594No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG12170.1300441530353609No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG11650.12448762389991408No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC11290.12064079603691245No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10840.1158322612081604No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10500.1121991460042144No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA10500.1121991460042144No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10230.1093140251069632No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG10070.10760432383451803No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.10450549027821113No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA9710.10375749597151637No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9520.10172722571048774No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9410.10055180608568168No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9400.10044494975615385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAC400.001930920323.12523
GTATCAA24600.022.936991
GGTATCA9000.022.8166681
TCGCGCG1151.3460522E-1020.9130449
CGTGCGA450.00382528520.55555532
GCCGAAC450.00382528520.55555511
CGCACGG450.00382528520.55555525
AATACGC450.00382528520.5555555
AGTACGC450.00382528520.55555521
TATAGGA1303.274181E-1119.9230772
ATCGCGC1600.019.656258
TAGGACC2750.019.5090924
CCGTCCG951.6752892E-719.47368428
GGACCGT951.6752892E-719.4736846
GCCGGCA1052.2580934E-819.3809515
AACGGCC4200.018.94047537
GTCAACC4200.018.94047515
CGCGCGC1306.9667294E-1018.510
TGTCCGG1501.2732926E-1118.514
CCGACAT500.0070339318.49999828
Produced by FastQC (version 0.11.5)