##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633472.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	935836
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.877655914070413	33.0	33.0	33.0	33.0	33.0
2	31.999761710385155	33.0	33.0	33.0	33.0	33.0
3	31.715442663030704	33.0	33.0	33.0	27.0	33.0
4	31.997516658901773	33.0	33.0	33.0	33.0	33.0
5	32.09582020781419	33.0	33.0	33.0	33.0	33.0
6	35.27307135010835	37.0	37.0	37.0	33.0	37.0
7	35.41406400266713	37.0	37.0	37.0	33.0	37.0
8	35.546660953414914	37.0	37.0	37.0	33.0	37.0
9	35.66456622741592	37.0	37.0	37.0	33.0	37.0
10	35.66483123111314	37.0	37.0	37.0	33.0	37.0
11	35.685847734004675	37.0	37.0	37.0	33.0	37.0
12	35.68394462277578	37.0	37.0	37.0	33.0	37.0
13	35.69140319457683	37.0	37.0	37.0	33.0	37.0
14	35.66148342230904	37.0	37.0	37.0	33.0	37.0
15	35.67616761911275	37.0	37.0	37.0	33.0	37.0
16	35.67023602426066	37.0	37.0	37.0	33.0	37.0
17	35.65209823088661	37.0	37.0	37.0	33.0	37.0
18	35.64070093477917	37.0	37.0	37.0	33.0	37.0
19	35.628343000269275	37.0	37.0	37.0	33.0	37.0
20	35.63795258998371	37.0	37.0	37.0	33.0	37.0
21	35.64421116520416	37.0	37.0	37.0	33.0	37.0
22	35.53426989344287	37.0	37.0	37.0	33.0	37.0
23	35.58698105223565	37.0	37.0	37.0	33.0	37.0
24	35.57998516834146	37.0	37.0	37.0	33.0	37.0
25	35.608204856406466	37.0	37.0	37.0	33.0	37.0
26	35.49145683645425	37.0	37.0	37.0	33.0	37.0
27	35.50989382755098	37.0	37.0	37.0	33.0	37.0
28	35.528698404421284	37.0	37.0	37.0	33.0	37.0
29	35.542432648455495	37.0	37.0	37.0	33.0	37.0
30	35.546636376459126	37.0	37.0	37.0	33.0	37.0
31	35.53457336541873	37.0	37.0	37.0	33.0	37.0
32	35.529276497164034	37.0	37.0	37.0	33.0	37.0
33	35.52381613872516	37.0	37.0	37.0	33.0	37.0
34	35.50043917951436	37.0	37.0	37.0	33.0	37.0
35	35.43345201509666	37.0	37.0	37.0	33.0	37.0
36	35.466368038844415	37.0	37.0	37.0	33.0	37.0
37	35.457259605315464	37.0	37.0	37.0	33.0	37.0
38	35.43856402190127	37.0	37.0	37.0	33.0	37.0
39	35.34773934749251	37.0	37.0	37.0	33.0	37.0
40	35.13918678058976	37.0	37.0	37.0	33.0	37.0
41	35.30558559405708	37.0	37.0	37.0	33.0	37.0
42	35.33954346701773	37.0	37.0	37.0	33.0	37.0
43	35.021005817258576	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	41.0
16	48.0
17	48.0
18	29.0
19	29.0
20	58.0
21	143.0
22	401.0
23	866.0
24	1868.0
25	3364.0
26	5588.0
27	8631.0
28	12190.0
29	16671.0
30	22161.0
31	28625.0
32	37327.0
33	48623.0
34	71180.0
35	144838.0
36	533103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.426061831346516	20.65255023316051	13.69598946824016	24.22539846725281
2	16.109125957966995	21.47619882116097	39.19094798661304	23.223727234258995
3	17.491846862057027	27.82079338687548	30.0311165631585	24.656243187908995
4	12.116011779841768	17.979752862681067	40.41114041349125	29.493094943985916
5	13.34603498903654	37.61064972922606	35.54661286806663	13.496702413670771
6	28.80932129133737	40.59963497877833	17.3792202907347	13.211823439149594
7	26.26175953906454	32.45739638141726	23.6800037613428	17.600840318175408
8	24.353732919015727	35.74654106061318	20.471856179928963	19.427869840442128
9	26.072837548459344	15.233224624827427	20.475596151462437	38.21834167525079
10	15.41765864959245	27.732957484003606	33.69051842416834	23.158865442235605
11	33.46911210938669	24.05015408682718	23.97257639158998	18.508157412196155
12	23.245205356494086	26.272124603028736	29.68714603840844	20.79552400206874
13	27.400741155501606	22.59316803371531	26.25331788903184	23.752772921751248
14	21.945404964117646	21.088203488645448	28.233686244170986	28.73270530306592
15	24.183510786077903	28.359242431366177	25.30325826319996	22.153988519355956
16	22.695322684743907	28.369928064318962	25.353480738078037	23.58126851285909
17	22.15676678392368	27.47789142542069	26.376950662295528	23.9883911283601
18	22.674378844156454	27.135630601943078	28.284870426014812	21.905120127885656
19	24.952983215007755	25.90165370855577	27.79525472411833	21.35010835231814
20	23.989245978996323	26.141332455686676	28.029697511102373	21.839724054214628
21	23.091332241974023	27.06211344722793	27.861398792096054	21.985155518701998
22	22.51751375240961	26.99265683303485	27.712227356075207	22.77760205848033
23	22.44891198885275	26.93912181194141	28.438957253193937	22.173008946011908
24	22.972935428857195	27.503430088177844	26.640992652558783	22.882641830406182
25	22.851119213195474	26.962416491778473	28.08782735436551	22.098636940660544
26	23.035660094290026	27.275398680965466	28.004799986322386	21.684141238422118
27	23.084386580554714	27.009540133100245	27.02556858252942	22.880504703815628
28	22.112528263499158	26.772960219525643	28.48597403818618	22.628537478789017
29	24.1124513269419	26.644198342444614	27.218764826315724	22.02458550429776
30	23.33528524228604	26.29189302399138	28.125440782359302	22.247380951363276
31	22.890869767779826	26.581794246000367	28.042306557986656	22.48502942823315
32	21.637979304066096	26.50410969443364	28.395039301758	23.462871699742262
33	21.77571711282746	26.755008356164968	28.739330395496648	22.729944135510923
34	22.852080920161224	26.530289495167956	28.417372274629315	22.2002573100415
35	22.760184476767297	27.334383374864828	27.626956005112007	22.278476143255872
36	23.65393081693801	27.037002209788895	27.158070431143916	22.150996542129175
37	22.67705025239465	26.465427703144567	27.520954526220404	23.336567518240376
38	23.064190734273954	26.84519509828645	27.425745536611117	22.664868630828476
39	22.18903739544108	26.886334785154663	27.96045460956834	22.964173209835913
40	22.653862428887113	27.503323231848313	28.051175633337465	21.791638705927106
41	22.369197167024993	26.2132467654589	28.128005334267968	23.28955073324813
42	21.951816343889313	27.590945422061132	27.767899503759207	22.689338730290352
43	23.139845015579652	25.934458601720817	27.67536192238811	23.25033446031142
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	73.0
2	90.0
3	338.0
4	586.0
5	586.0
6	699.0
7	812.0
8	741.5
9	671.0
10	982.5
11	1294.0
12	1294.0
13	1981.5
14	2669.0
15	4706.5
16	6744.0
17	7648.0
18	8552.0
19	8552.0
20	7777.0
21	7002.0
22	7347.5
23	7693.0
24	9825.0
25	11957.0
26	11957.0
27	15250.5
28	18544.0
29	21912.5
30	25281.0
31	30209.5
32	35138.0
33	35138.0
34	43452.0
35	51766.0
36	55940.0
37	60114.0
38	64461.0
39	68808.0
40	68808.0
41	71967.5
42	75127.0
43	76919.5
44	78712.0
45	76322.0
46	73932.0
47	73932.0
48	70521.5
49	67111.0
50	67376.0
51	67641.0
52	63867.0
53	60093.0
54	60093.0
55	59025.5
56	57958.0
57	49277.0
58	40596.0
59	36748.5
60	32901.0
61	32901.0
62	29261.5
63	25622.0
64	20560.5
65	15499.0
66	12871.5
67	10244.0
68	10244.0
69	8546.0
70	6848.0
71	5955.5
72	5063.0
73	4954.5
74	4846.0
75	4846.0
76	4077.0
77	3308.0
78	2404.5
79	1501.0
80	966.5
81	432.0
82	432.0
83	348.0
84	264.0
85	228.0
86	192.0
87	154.0
88	116.0
89	116.0
90	79.5
91	43.0
92	25.5
93	8.0
94	5.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	935836.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.385402391269004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.92587800869767	41.94098658891358
2	8.559269327407106	8.454059198184929
3	2.3385876686743936	3.4647627913423378
4	0.9834204600161431	1.9426646055081642
5	0.5771863495125749	1.4252290062713069
6	0.3839243745187283	1.1376155834054198
7	0.26389856781835414	0.9122915873532309
8	0.19581760703271026	0.7736425054884626
9	0.17208153396879527	0.7648484219240202
>10	1.270556882074161	12.646650259952935
>50	0.1738092524921497	6.070281324664844
>100	0.14040848787875185	13.498547610792693
>500	0.011645774422409215	3.9913024013685523
>1k	0.0032959738931346836	2.4125128459592085
>5k	2.1973159287564552E-4	0.5646052688703563
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5203	0.5559734825332644	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3701	0.39547527558247386	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2079	0.22215430908834455	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1818	0.19426480708158267	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1725	0.1843271684354951	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1489	0.1591090746669288	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1416	0.15130856261139772	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1279	0.13666924546608594	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1217	0.1300441530353609	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1165	0.12448762389991408	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1129	0.12064079603691245	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1084	0.1158322612081604	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1050	0.1121991460042144	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1050	0.1121991460042144	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1023	0.1093140251069632	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1007	0.10760432383451803	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	978	0.10450549027821113	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	971	0.10375749597151637	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	952	0.10172722571048774	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	941	0.10055180608568168	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	940	0.10044494975615385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.27425318111293E-4	0.0	0.0
2	0.0	0.0	4.27425318111293E-4	0.0	0.0
3	0.0	0.0	5.342816476391162E-4	0.0	0.0
4	0.0	0.0	8.54850636222586E-4	0.0	0.0
5	0.0	0.0	0.0010685632952782324	0.0	0.0
6	0.0	0.0	0.002243982920084288	0.0	0.0
7	0.0	0.0	0.0037399715334738137	1.0685632952782325E-4	0.0
8	0.0	0.0	0.004381109510640753	1.0685632952782325E-4	0.0
9	0.0	0.0	0.005770241794502456	7.479943066947628E-4	0.0
10	2.137126590556465E-4	0.0	0.0066250924307250415	7.479943066947628E-4	0.0
11	2.137126590556465E-4	0.0	0.010258207634671033	7.479943066947628E-4	0.0
12	2.137126590556465E-4	0.0	0.011006201941365795	7.479943066947628E-4	0.0
13	2.137126590556465E-4	0.0	0.012288477895699673	7.479943066947628E-4	0.0
14	2.137126590556465E-4	0.0	0.01282275954333879	8.54850636222586E-4	0.0
15	2.137126590556465E-4	0.0	0.014746173474839608	9.617069657504092E-4	0.0
16	2.137126590556465E-4	0.0	0.01645587474728478	9.617069657504092E-4	0.0
17	3.205689885834698E-4	0.0	0.01677644373586825	0.0010685632952782324	0.0
18	4.27425318111293E-4	0.0	0.017310725383507367	0.0011754196248060557	0.0
19	5.342816476391162E-4	0.0	0.017524438042563012	0.0011754196248060557	0.0
20	5.342816476391162E-4	0.0	0.017845007031146484	0.0013891322838617023	0.0
21	5.342816476391162E-4	0.0	0.018379288678785598	0.001709701272445172	0.0
22	5.342816476391162E-4	0.0	0.018486145008313422	0.0023508392496121113	0.0
23	6.411379771669396E-4	0.0	0.018486145008313422	0.002457695579139935	0.0
24	6.411379771669396E-4	0.0	0.018486145008313422	0.0032056898858346975	0.0
25	6.411379771669396E-4	0.0	0.01869985766736907	0.003419402544890344	0.0
26	6.411379771669396E-4	0.0	0.01869985766736907	0.0036331152039459904	0.0
27	6.411379771669396E-4	0.0	0.01869985766736907	0.005129103817335516	0.0
28	6.411379771669396E-4	0.0	0.01869985766736907	0.013677610179561376	0.0
29	6.411379771669396E-4	0.0	0.01869985766736907	0.03237746784693044	0.0
30	6.411379771669396E-4	0.0	0.01869985766736907	0.06058753884227578	0.0
31	6.411379771669396E-4	0.0	0.01869985766736907	0.12138879034360721	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCAC	40	0.0019309203	23.125	23
GTATCAA	2460	0.0	22.93699	1
GGTATCA	900	0.0	22.816668	1
TCGCGCG	115	1.3460522E-10	20.913044	9
CGTGCGA	45	0.003825285	20.555555	32
GCCGAAC	45	0.003825285	20.555555	11
CGCACGG	45	0.003825285	20.555555	25
AATACGC	45	0.003825285	20.555555	5
AGTACGC	45	0.003825285	20.555555	21
TATAGGA	130	3.274181E-11	19.923077	2
ATCGCGC	160	0.0	19.65625	8
TAGGACC	275	0.0	19.509092	4
CCGTCCG	95	1.6752892E-7	19.473684	28
GGACCGT	95	1.6752892E-7	19.473684	6
GCCGGCA	105	2.2580934E-8	19.38095	15
AACGGCC	420	0.0	18.940475	37
GTCAACC	420	0.0	18.940475	15
CGCGCGC	130	6.9667294E-10	18.5	10
TGTCCGG	150	1.2732926E-11	18.5	14
CCGACAT	50	0.00703393	18.499998	28
>>END_MODULE