FastQCFastQC Report
Fri 10 Feb 2017
ERR1633471.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633471.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078183
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT135271.2546107664468833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT94060.8723936474605888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54210.5027903426412771No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA37640.34910585679796474No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA34130.31655108641111945No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG31240.28974673130628104No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA25520.23669451289808874No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC25360.23521053476079665No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG23390.21693905394538776No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC23020.2135073545028998No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.21044664959473486No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG22350.20729319605298913No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG20930.19412289008452183No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT20850.1933809010158758No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC20740.19236066604648747No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA20560.19069119064203385No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG19200.1780773764750511No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT19050.17668614697133975No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC18810.17446017976540162No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT18330.17000824535352532No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT17520.16249560603348412No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC17340.1608261306290305No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC16830.15609595031641196No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG16650.15442647491195838No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG16640.1543337262783776No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT15610.14478061701955977No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG15490.1436676334165907No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA15420.14301839298152538No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA15400.1428328957143639No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA15120.14023593397410272No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA15040.13949394490545666No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT14970.13884470447039138No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT14850.1377317208674223No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT14830.1375462236002608No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG14660.13596949682938797No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14560.1350420104935804No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT14500.13448551869209585No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC14470.1342072727913536No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC14370.13327978645554603No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC14310.1327232946540615No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG14080.13059007608170414No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC14050.13031183018096185No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT13950.12938434384515432No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA13730.12734387390637766No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA13580.12595264440266632No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT13540.12558164986834333No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG13540.12558164986834333No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG13510.12530340396760103No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC13060.12112971545646704No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.12001673185349797No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT12910.11973848595275571No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12710.11788351328114058No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG12420.11519380290729868No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC12360.11463731110581414No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA12210.1132460816021028No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12210.1132460816021028No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG12100.11222584663271448No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT11970.11102011439616466No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA11960.1109273657625839No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG11910.11046362259468012No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG11840.10981438215961484No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG11590.10749566632009594No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTAC11560.10721742041935368No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11480.10647543135070762No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA11450.10619718544996537No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC11450.10619718544996537No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG11350.1052696991141578No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT11320.10499145321341555No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC11230.10415671551118873No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA11220.10406396687760798No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTT11200.10387846961044647No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10970.10174525103808908No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGC10880.10091051333586228No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG10880.10091051333586228No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC10860.10072501606870077No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT10860.10072501606870077No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT10830.10044677016795851No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG200.001841885537.01
ATTCACG200.001841885537.022
ATCCACA200.001841885537.025
GCGCTAC200.001841885537.031
GGTCGCT555.002221E-1033.63636428
TCTCGCG652.9904186E-928.46153820
GTCCGCT652.9904186E-928.46153810
GGTATCA23250.027.5311811
TGCTCTC754.620233E-1027.13333317
AGATCTT358.8690384E-426.428578
GGTCCGC706.5920176E-926.428579
ATAGATC358.8690384E-426.428576
CGCGAGG706.5920176E-926.4285723
GACGGTC358.8690384E-426.4285720
GTGACAT358.8690384E-426.4285716
TAGTTTC509.090407E-625.89999820
CCGCTCT5450.025.79816428
GCCGGCA5900.025.39830615
GCTCTCT5600.025.10714130
CGCTCTC5700.024.99122829
Produced by FastQC (version 0.11.5)