FastQCFastQC Report
Fri 10 Feb 2017
ERR1633470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073293
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37230.346876388833245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.2415929294237454No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC18180.16938524708537184No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG16240.15131003370002413No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC15010.13984997572890162No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC14690.1368684972323494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14460.1347255595629525No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13480.12559478166726143No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG13200.12298598798277822No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG11740.10938299234225883No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA11030.10276783692803364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.022.8529421
TTCGTAA450.00382564520.55555737
ATACCGT450.00382564520.5555576
TACACCG555.142789E-420.1818185
GTATCAA23200.019.9353451
AACGATT1254.129106E-1019.2422
TAGCGAA701.2191015E-418.510
TTACCGA801.6165075E-518.510
TATACTG1655.4569682E-1116.8181825
CGAACCG1901.8189894E-1216.55263131
AACGGCC4150.016.49397737
ATAAGAC904.445514E-516.4444453
TAACGGC4200.016.29761936
CCGACGC803.3823034E-416.187513
TTAACGG4300.015.91860435
AGGACCG1403.471905E-815.8571435
ATCCGTA700.00259234315.85714310
GCGGCAA700.00259234315.85714336
CCGCCTA700.00259234315.85714315
CCGTTAA3550.015.63380216
Produced by FastQC (version 0.11.5)