FastQCFastQC Report
Fri 10 Feb 2017
ERR1633469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933927
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT217482.3286616619928537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT161361.7277581652527445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90010.9637798243331653No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT48700.5214540322744712No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA47510.5087121370299821No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA43880.4698440027967925No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG43070.4611709480505436No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC42720.45742333180216443No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG38060.40752649832374477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37460.40110201332652334No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA34890.373583802588425No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA34560.3700503358399532No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA30750.32925485610759725No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC28220.3021649443693136No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC27880.29852440287088816No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG26480.2835339378773716No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC25690.27507503263103006No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT25290.2707920426328825No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT23130.24766389664288538No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA23090.24723559764307063No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC23060.2469143733932095No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22480.2407040378958955No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA21970.23524322564825734No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC21900.23449370239858147No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT21420.22935411440080433No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG20260.2169334434061763No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT19570.20954528565937167No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA19410.2078320896601126No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC19020.2036561744119187No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC18740.20065808141321537No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA18720.200443931913308No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG18210.1949831196656698No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT18040.19316284891645707No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC17940.19209210141692018No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA17610.18855863466844838No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA17130.18341904667067127No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA17060.18266952342099543No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16970.1817058506714122No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC16890.1808492526717827No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG16840.18031387892201425No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA16830.18020680417206056No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA16740.17924313142247736No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC16440.17603088892386665No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC16260.17410354342470022No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16150.17292572117520963No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG16040.17174789892571904No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC15940.17067715142618212No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG15850.16971347867659892No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA15810.16928517967678416No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC15660.16767905842747882No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT15650.1675719836775251No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT15640.16746490892757143No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG15430.16521633917854392No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG15060.1612545734302574No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC14880.15932722793109097No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT14820.15868477943136883No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA14810.15857770468141516No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14790.15836355518150777No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA14400.15418763993331383No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG14210.15215321968419374No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC13910.14894097718558302No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG13880.14861975293572197No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG13290.14230234268845424No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT13120.1404820719392415No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA13110.1403749971892878No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13060.13983962343951936No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT13040.13962547393961197No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG13000.13919717493979722No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG12970.13887595068993616No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG12970.13887595068993616No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG12940.13855472644007508No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG12910.13823350219021402No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG12850.13759105369049188No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG12800.13705567994072343No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG12620.13512833444155697No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA12510.1339505121920664No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12490.13373636269215902No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC12410.13287976469252952No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG12230.13095241919336306No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC12230.13095241919336306No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.12934629794405772No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12000.12848969994442822No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA11930.12774017669475238No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA11880.12720480294498393No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA11440.12249351394702154No Hit
GTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCG11440.12249351394702154No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG11180.11970957044822562No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG11180.11970957044822562No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11030.11810344919892025No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC10860.11628317844970752No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT10790.11553365520003168No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC10420.11157188945174516No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT10410.11146481470179148No Hit
GAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGT10320.11050114195220825No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG10190.1091091702028103No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC10160.1087879459529492No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10100.10814549745322707No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA10070.10782427320336602No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC9990.10696767520373647No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC9930.10632522670401434No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.10493325495461636No Hit
GATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACG9780.10471910545470897No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC9750.10439788120484791No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG9710.10396958220503316No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT9710.10396958220503316No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA9690.10375543270512579No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG9620.10300590945544995No Hit
GTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGC9590.10268468520558888No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG9540.10214931145582042No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT9530.10204223670586673No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9520.10193516195591305No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA9510.10182808720595934No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC9440.10107856395628351No Hit
CTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAA9420.10086441445637613No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA9400.10065026495646877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCAA200.001841759137.01
CTTCCCT352.387832E-531.71428735
CTATACC250.00549582229.64
ACGATCG250.00549582229.616
ATAGTAT250.00549582229.622
TAGTATT250.00549582229.623
TATTATA950.029.2105272
GACATCT950.029.21052718
GGATCTC454.007199E-628.7777798
CGATTTA454.007199E-628.77777917
CCTAACC454.007199E-628.77777929
CTAACCA454.007199E-628.77777930
ACCTAAC454.007199E-628.77777928
TCTCACC454.007199E-628.77777911
GATCTCA454.007199E-628.7777799
CCAAACT454.007199E-628.77777934
ATTATAG1050.028.1904753
ACTAGTG2000.027.7500028
TGGTGAC905.456968E-1226.72222314
CTAGTGC1250.026.649
Produced by FastQC (version 0.11.5)