FastQCFastQC Report
Fri 10 Feb 2017
ERR1633468.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633468.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842611
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32190.3820268190185032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22250.26406016536693677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13340.15831742049415448No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8570.10170766818852353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.027.0597021
GTATCAA18800.021.8457451
CCTACCG1001.2885721E-820.3524
CTACCGA1101.7535058E-920.18181825
CCTATAG2000.019.4250012
CGAACCG1900.018.531
CTAGTAC500.007033361618.53
CTAGCAC500.007033361618.53
TACCGAG1156.398841E-817.69565226
TGCGGTA953.6040765E-617.52631636
GTATATG2050.017.1463411
CGAGCCT1201.0401891E-716.95833429
GCGAACC2100.016.73809430
ATTCGGA1005.8740443E-616.65000236
AACCGGG2150.016.34883733
CCTAGTC803.3812248E-416.18752
CCGCCGT803.3812248E-416.187524
CTTGCGG1201.9339095E-615.41666734
ATAATAT2550.015.2352943
GTCTATA855.362042E-415.2352931
Produced by FastQC (version 0.11.5)