FastQCFastQC Report
Fri 10 Feb 2017
ERR1633467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776880
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54120.6966326845844919No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37390.4812841108021831No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA21200.2728864174647307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20690.2663216970445886No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA10890.14017608897126968No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10140.13052208835341367No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9670.12447224796622387No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC8950.11520440737308207No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG8070.10387704664813098No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8000.10297600659046442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGT250.005495259529.626
GGTATCA9650.026.455961
GTATCAA26050.024.1458721
AACGATT400.001930626723.12522
GCTTTAT4300.019.7906971
TTCGCCG1500.019.73333424
CTGACGC851.2442451E-619.58823627
TTAACGG1052.2566383E-819.3809535
TTATTGA4550.019.1098924
AACGGCC1156.397022E-817.69565237
TAACCGT953.6033434E-617.5263167
GTATTAG2750.017.490911
GTGAGTA752.0661796E-417.2666662
GGATCCG1401.8662831E-917.17857219
TATTGAG5100.017.049025
GCCGGCA1757.2759576E-1216.91428615
TAGCCGG2300.016.89130430
CCGCTCT1655.4569682E-1116.81818228
GCCGCTC1655.4569682E-1116.81818227
TAGGACC2050.016.2439044
Produced by FastQC (version 0.11.5)