##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633467.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	776880
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.858550870147255	33.0	33.0	33.0	33.0	33.0
2	31.96740423231387	33.0	33.0	33.0	33.0	33.0
3	31.68717948717949	33.0	33.0	33.0	27.0	33.0
4	31.97161981258367	33.0	33.0	33.0	33.0	33.0
5	32.07212310781588	33.0	33.0	33.0	33.0	33.0
6	35.23171918443003	37.0	37.0	37.0	33.0	37.0
7	35.38147719081454	37.0	37.0	37.0	33.0	37.0
8	35.52321336628565	37.0	37.0	37.0	33.0	37.0
9	35.63439012460097	37.0	37.0	37.0	33.0	37.0
10	35.63043713314798	37.0	37.0	37.0	33.0	37.0
11	35.66475517454433	37.0	37.0	37.0	33.0	37.0
12	35.64985325919061	37.0	37.0	37.0	33.0	37.0
13	35.6473406446298	37.0	37.0	37.0	33.0	37.0
14	35.62324554628771	37.0	37.0	37.0	33.0	37.0
15	35.63844480486047	37.0	37.0	37.0	33.0	37.0
16	35.62679306971476	37.0	37.0	37.0	33.0	37.0
17	35.615151632169706	37.0	37.0	37.0	33.0	37.0
18	35.61895015961281	37.0	37.0	37.0	33.0	37.0
19	35.59270801153331	37.0	37.0	37.0	33.0	37.0
20	35.596718926990015	37.0	37.0	37.0	33.0	37.0
21	35.5989702399341	37.0	37.0	37.0	33.0	37.0
22	35.49582561013284	37.0	37.0	37.0	33.0	37.0
23	35.538049634435176	37.0	37.0	37.0	33.0	37.0
24	35.53031742354032	37.0	37.0	37.0	33.0	37.0
25	35.56078030068994	37.0	37.0	37.0	33.0	37.0
26	35.440416795386675	37.0	37.0	37.0	33.0	37.0
27	35.469547420451036	37.0	37.0	37.0	33.0	37.0
28	35.4820281124498	37.0	37.0	37.0	33.0	37.0
29	35.47743924415611	37.0	37.0	37.0	33.0	37.0
30	35.48440170940171	37.0	37.0	37.0	33.0	37.0
31	35.4641656369066	37.0	37.0	37.0	33.0	37.0
32	35.465795232210894	37.0	37.0	37.0	33.0	37.0
33	35.46486973535166	37.0	37.0	37.0	33.0	37.0
34	35.42603104726599	37.0	37.0	37.0	33.0	37.0
35	35.37259937184636	37.0	37.0	37.0	33.0	37.0
36	35.395223200494286	37.0	37.0	37.0	33.0	37.0
37	35.38326768612913	37.0	37.0	37.0	33.0	37.0
38	35.37143574297189	37.0	37.0	37.0	33.0	37.0
39	35.25876325816085	37.0	37.0	37.0	33.0	37.0
40	35.0499485119967	37.0	37.0	37.0	33.0	37.0
41	35.215342137781896	37.0	37.0	37.0	33.0	37.0
42	35.25047111523015	37.0	37.0	37.0	33.0	37.0
43	34.90749407887962	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	23.0
16	47.0
17	41.0
18	28.0
19	37.0
20	40.0
21	126.0
22	331.0
23	848.0
24	1719.0
25	2997.0
26	4954.0
27	7483.0
28	10717.0
29	14492.0
30	19034.0
31	24820.0
32	31565.0
33	41487.0
34	59658.0
35	121129.0
36	435297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.173308619091756	20.283312738132015	14.05519513953249	23.488183503243746
2	15.95767686129132	22.042271650705388	38.76789208114509	23.2321594068582
3	18.18813716404078	27.591519925857277	30.461589949541757	23.75875296056019
4	12.251441664092265	17.82385954072701	40.6290546802595	29.29564411492122
5	13.784110802183092	36.88652044073731	35.177504891360314	14.151863865719289
6	28.573396148697356	40.78055812995572	17.13649469673566	13.509551024611266
7	25.922150139017607	32.69912985274431	23.42408093914118	17.9546390690969
8	24.863299351251158	34.49657604778087	20.945963340541653	19.69416126042632
9	26.572186180619916	15.247786015858306	20.240963855421686	37.93906394810009
10	15.212645453609309	28.314540212130574	33.21812892596025	23.254685408299867
11	33.64393471321182	24.426938523324065	24.025718257645966	17.903408505818145
12	23.83843064565956	25.8677015755329	29.77551230563279	20.51835547317475
13	28.681392235609103	22.46833487797343	26.028987745855215	22.82128514056225
14	22.347981670270826	21.347569766244465	28.491272783441456	27.81317578004325
15	24.02842137781897	28.750128720008238	25.04762640304809	22.173823499124705
16	22.16944701884461	28.542503346720217	25.754942848316343	23.533106786118836
17	22.4692359180311	27.175754299248272	26.41270209041293	23.942307692307693
18	23.293558850787765	26.317578004324993	28.548167027082687	21.84069611780455
19	25.59700339820822	25.673463083101637	28.277211409741533	20.452322108948614
20	25.381268664401198	25.50715683245804	27.605679126763466	21.505895376377303
21	23.358047574915044	26.563948100092677	28.028910513850274	22.049093811142004
22	22.612115127175368	26.76642467305118	28.4227937390588	22.19866646071465
23	22.762460096797447	26.239573679332718	28.524997425599835	22.472968798270003
24	22.407450314076822	26.50010297600659	28.299866131191433	22.792580578725158
25	23.04976315518484	26.894114921223355	28.194701884460923	21.861420039130884
26	23.154927401915355	26.775692513644323	27.959015549376996	22.110364535063333
27	23.11631139944393	26.36250128720008	27.939321388116568	22.58186592523942
28	22.617907527546084	26.680439707548143	28.06598187622284	22.635670888682938
29	23.363839975285757	26.847260838224695	27.707496653279783	22.08140253320976
30	23.68164967562558	26.26596128102152	27.819483060446913	22.232905982905983
31	22.67261353104727	26.631912264442388	28.23705076717125	22.4584234373391
32	21.9098187622284	26.696400988569664	28.36371125527752	23.030068993924417
33	21.802981155390793	26.90492740191535	28.68216455565853	22.60992688703532
34	23.081685717227888	26.12591391205849	28.185047883843062	22.60735248687056
35	22.517892081145092	26.71866954999485	27.816265060240962	22.947173308619092
36	22.789362578519203	26.78650499433632	28.244259087632585	22.179873339511893
37	23.08940891772217	26.200314076820103	28.31762949232829	22.39264751312944
38	23.195345484502113	26.499974255998353	27.766965297085775	22.53771496241376
39	22.90649778601586	26.353490886623415	27.96673874987128	22.773272577489443
40	22.56281536402018	26.754453712285038	28.253526928225725	22.429203995469056
41	22.454819277108435	26.083436309339923	28.218901246009683	23.242843167541963
42	22.061322211924622	26.896174441355164	28.288796210482957	22.753707136237256
43	22.918340026773762	26.649546905571004	27.88976418494491	22.54234888271033
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	81.0
2	93.0
3	329.0
4	565.0
5	565.0
6	680.5
7	796.0
8	729.5
9	663.0
10	946.0
11	1229.0
12	1229.0
13	1986.0
14	2743.0
15	4779.5
16	6816.0
17	7845.0
18	8874.0
19	8874.0
20	8241.0
21	7608.0
22	8232.5
23	8857.0
24	11112.0
25	13367.0
26	13367.0
27	16329.5
28	19292.0
29	22325.0
30	25358.0
31	28478.5
32	31599.0
33	31599.0
34	36027.0
35	40455.0
36	42901.0
37	45347.0
38	47176.0
39	49005.0
40	49005.0
41	50856.5
42	52708.0
43	53413.0
44	54118.0
45	54604.0
46	55090.0
47	55090.0
48	55364.0
49	55638.0
50	55504.5
51	55371.0
52	53742.5
53	52114.0
54	52114.0
55	49060.5
56	46007.0
57	41260.5
58	36514.0
59	34437.0
60	32360.0
61	32360.0
62	28878.5
63	25397.0
64	21513.5
65	17630.0
66	14866.0
67	12102.0
68	12102.0
69	10262.0
70	8422.0
71	7054.0
72	5686.0
73	4062.0
74	2438.0
75	2438.0
76	1726.0
77	1014.0
78	767.5
79	521.0
80	431.0
81	341.0
82	341.0
83	298.5
84	256.0
85	227.5
86	199.0
87	167.0
88	135.0
89	135.0
90	97.5
91	60.0
92	41.0
93	22.0
94	11.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	776880.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.172600074800094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.84465048402183	51.10151721317596
2	7.159639174291033	8.329896527318084
3	1.6876613841234263	2.945269522808869
4	0.7683173326685265	1.7878006769545332
5	0.4650505246418208	1.35265990922823
6	0.3000795187227401	1.0473843499977866
7	0.21936683514558264	0.8932797419419005
8	0.17202516868405432	0.8005721072526013
9	0.1437115143851942	0.7524065207226404
>10	0.997331398951812	11.44728374062956
>50	0.14337366143818667	5.748628696294307
>100	0.09386455380042352	10.417791207189262
>500	0.0035843266365980253	1.3630011912925863
>1k	0.0011201020739368829	1.3072248733010743
>5k	2.2402041478737658E-4	0.7052837218926741
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5412	0.6966326845844919	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3739	0.4812841108021831	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2120	0.2728864174647307	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2069	0.2663216970445886	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1089	0.14017608897126968	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1014	0.13052208835341367	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	967	0.12447224796622387	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	895	0.11520440737308207	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	807	0.10387704664813098	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	800	0.10297600659046442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.5744001647616104E-4	0.0	0.0
2	0.0	0.0	2.5744001647616104E-4	0.0	0.0
3	0.0	0.0	2.5744001647616104E-4	1.2872000823808052E-4	0.0
4	0.0	0.0	6.436000411904027E-4	2.5744001647616104E-4	0.0
5	0.0	0.0	7.723200494284832E-4	2.5744001647616104E-4	0.0
6	0.0	0.0	0.0018020801153331273	2.5744001647616104E-4	0.0
7	0.0	0.0	0.004247760271856658	5.148800329523221E-4	0.0
8	0.0	0.0	0.0050200803212851405	5.148800329523221E-4	0.0
9	0.0	0.0	0.006178560395427865	0.0015446400988569664	0.0
10	0.0	0.0	0.006564720420142107	0.0016733601070950468	0.0
11	1.2872000823808052E-4	0.0	0.010297600659046443	0.0019308001235712078	0.0
12	1.2872000823808052E-4	0.0	0.011327360724951087	0.0019308001235712078	0.0
13	1.2872000823808052E-4	0.0	0.01197096076614149	0.0020595201318092883	0.0
14	1.2872000823808052E-4	0.0	0.013258160848522294	0.002188240140047369	0.0
15	1.2872000823808052E-4	0.0	0.013773040881474616	0.002188240140047369	0.0
16	1.2872000823808052E-4	0.0	0.015188960972093503	0.00244568015652353	0.0
17	1.2872000823808052E-4	0.0	0.015446400988569663	0.00244568015652353	0.0
18	1.2872000823808052E-4	0.0	0.015961281021521986	0.00244568015652353	0.0
19	1.2872000823808052E-4	0.0	0.01660488106271239	0.00244568015652353	0.0
20	1.2872000823808052E-4	0.0	0.017634641128617033	0.0025744001647616107	0.0
21	1.2872000823808052E-4	0.0	0.018020801153331274	0.0028318401812377717	0.0
22	1.2872000823808052E-4	0.0	0.018278241169807436	0.0033467202141900937	0.0
23	1.2872000823808052E-4	0.0	0.018278241169807436	0.003990320255380497	0.0
24	1.2872000823808052E-4	0.0	0.018278241169807436	0.0051488003295232215	0.0
25	2.5744001647616104E-4	0.0	0.018278241169807436	0.0054062403459993824	0.0
26	2.5744001647616104E-4	0.0	0.018278241169807436	0.0060498403871897845	0.0
27	2.5744001647616104E-4	0.0	0.018278241169807436	0.007980640510760993	0.0
28	2.5744001647616104E-4	0.0	0.018278241169807436	0.01737720111214087	0.0
29	2.5744001647616104E-4	0.0	0.018278241169807436	0.03758624240551951	0.0
30	2.5744001647616104E-4	0.0	0.018406961178045515	0.06461744413551643	0.0
31	2.5744001647616104E-4	0.0	0.018406961178045515	0.12962104829574708	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGGT	25	0.0054952595	29.6	26
GGTATCA	965	0.0	26.45596	1
GTATCAA	2605	0.0	24.145872	1
AACGATT	40	0.0019306267	23.125	22
GCTTTAT	430	0.0	19.790697	1
TTCGCCG	150	0.0	19.733334	24
CTGACGC	85	1.2442451E-6	19.588236	27
TTAACGG	105	2.2566383E-8	19.38095	35
TTATTGA	455	0.0	19.109892	4
AACGGCC	115	6.397022E-8	17.695652	37
TAACCGT	95	3.6033434E-6	17.526316	7
GTATTAG	275	0.0	17.49091	1
GTGAGTA	75	2.0661796E-4	17.266666	2
GGATCCG	140	1.8662831E-9	17.178572	19
TATTGAG	510	0.0	17.04902	5
GCCGGCA	175	7.2759576E-12	16.914286	15
TAGCCGG	230	0.0	16.891304	30
CCGCTCT	165	5.4569682E-11	16.818182	28
GCCGCTC	165	5.4569682E-11	16.818182	27
TAGGACC	205	0.0	16.243904	4
>>END_MODULE