FastQCFastQC Report
Fri 10 Feb 2017
ERR1633466.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633466.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601334
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34660.5763851703046893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24140.4014407966288286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13700.2278267984181836No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.11624155627321922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5300.026.877361
GTATCAA15350.024.3452761
CCGTCCG551.900703E-523.54545428
CGTCCGC603.7224832E-521.58333229
TATAGCC705.0952494E-621.14285731
GCCGTCC705.0952494E-621.14285727
TTCGCCG806.948758E-720.812524
GTCCGCC555.139174E-420.18181830
GCGATAG555.139174E-420.18181836
AGTTCGC656.895137E-519.9230778
CGAGTCG759.253728E-619.73333221
CGCCGTC851.2432884E-619.58823426
GTCGCCA609.2298345E-418.512
GGCGTCA500.007031075718.525
AGGACCG609.2298345E-418.55
ACACTAG500.007031075718.525
CGATAGA609.2298345E-418.537
CTGCTCG2350.018.1063849
ATAGTAC1351.1477823E-917.8148143
ACTGCTC2600.017.7884628
Produced by FastQC (version 0.11.5)