FastQCFastQC Report
Fri 10 Feb 2017
ERR1633465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938328
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56750.6047991747022363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40600.4326845196988686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22080.23531217228943396No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA18270.1947080338644909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.11190116888763843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG303.598979E-430.83333423
CGTTCGG250.005495834629.624
CGTCCTA358.8681746E-426.42857233
GGTATCA10650.024.6666661
TCGATCA400.001930924523.12534
GTATCAA28200.022.3705671
TCACGGT909.4736606E-820.5555551
ACGGTAC909.4736606E-820.5555553
TCGTATG759.2628E-619.73333237
CACGGTA951.6752892E-719.4736842
GCTTTAT4100.018.9512181
CTCGTAT701.2188941E-418.536
TTACTCG701.2188941E-418.519
CGATCAA701.2188941E-418.535
GTCGGCT902.1513588E-618.523
GGTCGGC953.6049587E-617.52631622
GCCGGCA1705.456968E-1217.41176415
CGGTCAC650.001579641417.07692330
ATTGAGC4450.017.0449436
CTAGCGC1005.875474E-616.65000228
Produced by FastQC (version 0.11.5)