FastQCFastQC Report
Fri 10 Feb 2017
ERR1633464.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633464.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573324
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33220.5794280372006055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24250.4229720018697979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14030.2447132860302377No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA13640.23791084971150692No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA7730.13482777626612527No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6670.11633910319470318No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC6620.11546699597435306No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG5740.10011790889619133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6500.027.6076931
GTATCAA17850.022.5938381
TTGCCGA705.0945655E-621.14285710
GACCGCA450.00382350520.55555515
CGACATT450.00382350520.55555530
GGCGTCA555.138773E-420.18181825
GCCGCTC555.138773E-420.18181827
TTTGCCG656.894421E-519.9230779
CCGAGTT851.243081E-619.58823613
TGCGGTA701.2178451E-418.536
ACATGCG609.2291174E-418.527
GATCTCG609.2291174E-418.536
TGCCGAG701.2178451E-418.511
CTTGCGG500.007030687318.49999834
ACAATAC500.007030687318.4999983
AGACCGC500.007030687318.49999814
CGCAACC500.007030687318.49999818
CGTCAAT500.007030687318.49999830
CGAGTTC852.7192615E-517.41176414
TTGTCCG852.7192615E-517.41176413