##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633464.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	573324
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.418601000481402	33.0	33.0	33.0	27.0	33.0
2	31.6283340659034	33.0	33.0	33.0	27.0	33.0
3	31.371547327514634	33.0	33.0	33.0	27.0	33.0
4	31.68127271839309	33.0	33.0	33.0	27.0	33.0
5	31.802980513636268	33.0	33.0	33.0	33.0	33.0
6	34.74718832632159	37.0	37.0	37.0	27.0	37.0
7	34.92828139062729	37.0	37.0	37.0	27.0	37.0
8	35.05398169272523	37.0	37.0	37.0	33.0	37.0
9	35.17687904221697	37.0	37.0	37.0	33.0	37.0
10	35.18802108406416	37.0	37.0	37.0	33.0	37.0
11	35.1959467944827	37.0	37.0	37.0	33.0	37.0
12	35.2013119981023	37.0	37.0	37.0	33.0	37.0
13	35.21425232503785	37.0	37.0	37.0	33.0	37.0
14	35.17989478898494	37.0	37.0	37.0	33.0	37.0
15	35.19709448758468	37.0	37.0	37.0	33.0	37.0
16	35.19158276995207	37.0	37.0	37.0	33.0	37.0
17	35.16971206508013	37.0	37.0	37.0	33.0	37.0
18	35.16416371894426	37.0	37.0	37.0	33.0	37.0
19	35.136341754400654	37.0	37.0	37.0	33.0	37.0
20	35.147858802352594	37.0	37.0	37.0	33.0	37.0
21	35.141930217468655	37.0	37.0	37.0	33.0	37.0
22	35.04543329775136	37.0	37.0	37.0	27.0	37.0
23	35.0869874625866	37.0	37.0	37.0	33.0	37.0
24	35.079578039642506	37.0	37.0	37.0	33.0	37.0
25	35.09980743872575	37.0	37.0	37.0	33.0	37.0
26	34.97159721204764	37.0	37.0	37.0	27.0	37.0
27	34.9871904891475	37.0	37.0	37.0	27.0	37.0
28	35.00605416832367	37.0	37.0	37.0	27.0	37.0
29	35.00620765919445	37.0	37.0	37.0	27.0	37.0
30	35.01007283839505	37.0	37.0	37.0	27.0	37.0
31	34.99035623835737	37.0	37.0	37.0	27.0	37.0
32	34.98538348298693	37.0	37.0	37.0	27.0	37.0
33	34.97394143625594	37.0	37.0	37.0	27.0	37.0
34	34.957111511117624	37.0	37.0	37.0	27.0	37.0
35	34.89445409576435	37.0	37.0	37.0	27.0	37.0
36	34.926476128681166	37.0	37.0	37.0	27.0	37.0
37	34.91667189930999	37.0	37.0	37.0	27.0	37.0
38	34.89612156476966	37.0	37.0	37.0	27.0	37.0
39	34.77518471230927	37.0	37.0	37.0	27.0	37.0
40	34.56697957873733	37.0	37.0	37.0	27.0	37.0
41	34.75200584660681	37.0	37.0	37.0	27.0	37.0
42	34.78490870781617	37.0	37.0	37.0	27.0	37.0
43	34.38923191772889	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	19.0
16	46.0
17	38.0
18	32.0
19	40.0
20	89.0
21	205.0
22	484.0
23	1127.0
24	2154.0
25	3631.0
26	5608.0
27	8069.0
28	10882.0
29	14376.0
30	18184.0
31	22646.0
32	28790.0
33	37375.0
34	53936.0
35	102833.0
36	262753.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.379568969727416	20.989004472165824	13.970808827120443	23.66061773098632
2	16.106599409757834	22.18396578548953	38.309751554095065	23.399683250657567
3	18.13145795396669	27.694288046549598	30.279388269111358	23.894865730372356
4	12.494505724511793	18.216924461561	40.096175984260206	29.192393829666997
5	13.936796645526789	37.00473030956318	35.513601384208584	13.544871660701455
6	28.8095387599333	40.70194165951539	17.19900091396837	13.289518666582945
7	25.99455107408725	32.67663659640971	23.65451298044387	17.674299349059172
8	24.795054803217727	35.20836385708604	20.75946585176968	19.23711548792655
9	25.962980792710578	15.524555050896177	20.508298972308854	38.00416518408439
10	14.910940410657847	28.618024014344417	33.70031605165666	22.770719523341075
11	33.597407399655346	24.413420683592523	24.12161360766338	17.867558309088754
12	23.278809189917045	26.15606533129609	30.076710551101993	20.488414927684868
13	28.164353838318295	22.52234338698537	26.204380071303486	23.108922703392846
14	21.837564797566472	21.373080492008008	28.591163111957634	28.19819159846788
15	23.802073522127102	29.211405767070627	24.92133592872442	22.06518478207785
16	22.32664252673881	28.753026212054618	25.70780222003614	23.212529041170438
17	22.316177240094607	27.796847855662765	26.567525517857266	23.31944938638536
18	22.77508005944283	27.067940640894157	28.664071275578905	21.492908024084112
19	25.080408285716278	26.15065826652992	28.223308286413967	20.545625161339835
20	24.609819229615365	26.151879216638413	27.85737209675506	21.38092945699116
21	23.035840118327506	26.74822613391381	28.266564804543332	21.949368943215354
22	22.41193461288905	27.109976208915025	28.416218403555405	22.061870774640518
23	22.54397164605005	26.582525762047286	28.562557995130156	22.310944596772504
24	22.316177240094607	27.087126999741855	27.92557088138644	22.671124878777096
25	22.71211391813355	27.441725795536204	28.05813117887966	21.788029107450587
26	22.904500770942782	27.201896309939926	28.046444942126964	21.84715797699032
27	22.738277134744052	26.955089966580854	27.7780103397032	22.52862255897189
28	22.358910493891763	26.94567120860107	28.424590632870768	22.2708276646364
29	23.31770517194466	27.29800252562251	27.661496815064428	21.722795487368398
30	23.386950485240458	26.631014923498753	27.962897070417426	22.019137520843362
31	22.498447649147778	26.974450746872623	28.27389050519427	22.25321109878533
32	21.80983178795934	27.0356726737412	28.404183323914577	22.750312214384884
33	21.589537504098903	27.197710195282248	28.826283218564026	22.386469082054823
34	23.05363110562265	26.42502319805206	28.193307798033924	22.328037898291367
35	22.422225478089178	27.005672185361156	27.841325323900623	22.730777012649042
36	22.760951922473158	27.130034674983083	28.01940961829611	22.08960378424765
37	22.834557771870706	26.51066412709044	28.10871339765996	22.546064703378892
38	23.083806015446765	26.783284844171884	27.793010583893224	22.339898556488127
39	22.573797712986025	26.93921761517048	28.06249171498141	22.42449295686209
40	22.400946061912634	27.066894112229733	28.276332405411253	22.25582742044638
41	22.206814994662704	26.464791287300027	28.18493556871856	23.14345814931871
42	22.048963587779337	27.20102420271958	28.18493556871856	22.565076640782525
43	22.80944108392462	26.81555281132483	27.957490005651252	22.417516099099288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	58.5
2	65.0
3	198.5
4	332.0
5	332.0
6	448.5
7	565.0
8	496.5
9	428.0
10	572.5
11	717.0
12	717.0
13	1197.0
14	1677.0
15	3118.0
16	4559.0
17	5232.5
18	5906.0
19	5906.0
20	5802.0
21	5698.0
22	6217.0
23	6736.0
24	8588.0
25	10440.0
26	10440.0
27	12526.0
28	14612.0
29	17037.5
30	19463.0
31	22112.5
32	24762.0
33	24762.0
34	28330.5
35	31899.0
36	33931.5
37	35964.0
38	37412.5
39	38861.0
40	38861.0
41	39969.0
42	41077.0
43	41270.5
44	41464.0
45	40937.5
46	40411.0
47	40411.0
48	39985.0
49	39559.0
50	39231.0
51	38903.0
52	37620.5
53	36338.0
54	36338.0
55	34479.0
56	32620.0
57	28864.0
58	25108.0
59	23915.5
60	22723.0
61	22723.0
62	20375.5
63	18028.0
64	15335.5
65	12643.0
66	10499.0
67	8355.0
68	8355.0
69	7053.5
70	5752.0
71	4881.0
72	4010.0
73	2849.0
74	1688.0
75	1688.0
76	1215.0
77	742.0
78	560.5
79	379.0
80	327.0
81	275.0
82	275.0
83	233.5
84	192.0
85	182.0
86	172.0
87	138.0
88	104.0
89	104.0
90	67.0
91	30.0
92	21.5
93	13.0
94	7.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	573324.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.465461332328054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.56180943872496	53.54930664138229
2	6.41848000987792	7.761927096991878
3	1.5762173829327535	2.8592013365719118
4	0.7808981561908351	1.8886946907057285
5	0.4881948749738796	1.4759464167686922
6	0.32856906985022477	1.1920248232816657
7	0.2369250397908403	1.0028047282493335
8	0.1775990101200602	0.8590884863259391
9	0.14144912308229823	0.7697507834001974
>10	1.0827021151927108	12.942644308327408
>50	0.12991125349906366	5.4687015746701
>100	0.07431859675832587	8.066383205868528
>500	0.001755557403739969	0.6572490593016944
>1k	0.0011703716024933125	1.5062768481546773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3322	0.5794280372006055	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2425	0.4229720018697979	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1403	0.2447132860302377	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1364	0.23791084971150692	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	773	0.13482777626612527	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	667	0.11633910319470318	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	662	0.11546699597435306	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	574	0.10011790889619133	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.4884288814003946E-4	0.0	0.0
2	0.0	0.0	3.4884288814003946E-4	0.0	0.0
3	0.0	0.0	3.4884288814003946E-4	0.0	0.0
4	0.0	0.0	5.232643322100592E-4	1.7442144407001973E-4	0.0
5	0.0	0.0	6.976857762800789E-4	1.7442144407001973E-4	0.0
6	0.0	0.0	0.0010465286644201184	1.7442144407001973E-4	0.0
7	0.0	0.0	0.0012209501084901383	1.7442144407001973E-4	0.0
8	0.0	0.0	0.0015697929966301777	1.7442144407001973E-4	0.0
9	0.0	0.0	0.0024419002169802765	1.7442144407001973E-4	0.0
10	0.0	0.0	0.0029651645491903357	1.7442144407001973E-4	0.0
11	0.0	0.0	0.005581486210240631	1.7442144407001973E-4	0.0
12	0.0	0.0	0.0059303290983806715	1.7442144407001973E-4	0.0
13	0.0	0.0	0.006279171986520711	1.7442144407001973E-4	0.0
14	0.0	0.0	0.00697685776280079	3.4884288814003946E-4	0.0
15	0.0	0.0	0.007674543539080869	3.4884288814003946E-4	0.0
16	0.0	0.0	0.008023386427220908	3.4884288814003946E-4	0.0
17	0.0	0.0	0.008372229315360948	3.4884288814003946E-4	0.0
18	0.0	0.0	0.008546650759430967	3.4884288814003946E-4	0.0
19	0.0	0.0	0.008721072203500987	3.4884288814003946E-4	0.0
20	0.0	0.0	0.008895493647571006	5.232643322100592E-4	0.0
21	0.0	0.0	0.009244336535711047	5.232643322100592E-4	0.0
22	0.0	0.0	0.009244336535711047	6.976857762800789E-4	0.0
23	0.0	0.0	0.009418757979781067	0.0013953715525601579	0.0
24	0.0	0.0	0.009593179423851086	0.0019186358847702173	0.0
25	0.0	0.0	0.009593179423851086	0.002616321661050296	0.0
26	0.0	0.0	0.009767600867921106	0.002616321661050296	0.0
27	0.0	0.0	0.009767600867921106	0.003314007437330375	0.0
28	1.7442144407001973E-4	0.0	0.009767600867921106	0.008895493647571006	0.0
29	1.7442144407001973E-4	0.0	0.009767600867921106	0.02267478772910257	0.0
30	1.7442144407001973E-4	0.0	0.009767600867921106	0.04098903935645464	0.0
31	1.7442144407001973E-4	0.0	0.009767600867921106	0.09035030802827022	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	650	0.0	27.607693	1
GTATCAA	1785	0.0	22.593838	1
TTGCCGA	70	5.0945655E-6	21.142857	10
GACCGCA	45	0.003823505	20.555555	15
CGACATT	45	0.003823505	20.555555	30
GGCGTCA	55	5.138773E-4	20.181818	25
GCCGCTC	55	5.138773E-4	20.181818	27
TTTGCCG	65	6.894421E-5	19.923077	9
CCGAGTT	85	1.243081E-6	19.588236	13
TGCGGTA	70	1.2178451E-4	18.5	36
ACATGCG	60	9.2291174E-4	18.5	27
GATCTCG	60	9.2291174E-4	18.5	36
TGCCGAG	70	1.2178451E-4	18.5	11
CTTGCGG	50	0.0070306873	18.499998	34
ACAATAC	50	0.0070306873	18.499998	3
AGACCGC	50	0.0070306873	18.499998	14
CGCAACC	50	0.0070306873	18.499998	18
CGTCAAT	50	0.0070306873	18.499998	30
CGAGTTC	85	2.7192615E-5	17.411764	14
TTGTCCG	85	2.7192615E-5	17.411764	13
>>END_MODULE