FastQCFastQC Report
Fri 10 Feb 2017
ERR1633463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804739
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78620.976962717104552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57600.7157600166016559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30220.3755254809323271No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC25240.313642062830309No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG24340.30245831257090805No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC23750.29512674295641195No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA21280.2644335616889451No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC19710.24492413068087915No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG18810.23374038042147824No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG18460.22939114420948903No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG18340.22789997750823557No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT18250.2267816024822955No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA17930.22280515794561967No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG17500.21746181059946143No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC17280.2147280049804968No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG16780.20851481039194075No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT16510.2051596853141205No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG15420.1916149211110683No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT15340.19062080997689934No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15160.18838405992501916No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC15100.18763847657439245No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT14680.18241939312000538No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA14630.18179807366114975No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14420.17918853193395623No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT13650.16962021226757992No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA13560.16850183724163983No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG13450.16713493443215752No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13400.1665136149733019No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC13390.16638935108153077No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC13140.16328275378725277No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT13020.16179158708599933No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG13000.16154305930245708No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG12830.15943057314234801No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA12780.1588092536834924No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG12470.15495707303858766No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT12440.1545842813632743No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC12010.1492409340171161No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT11800.14663139228992256No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA11760.1461343367228381No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC11630.14451890612981352No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11330.1407909893766799No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11330.1407909893766799No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG10910.13557190592229282No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG10870.13507485035520833No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC10730.13333515587041264No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA10700.1329623641950993No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT10350.12861312798311006No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT10290.12786754463248332No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC10210.12687343349831434No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC9970.12389110009580746No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT9910.12314551674518075No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC9790.12165435004392727No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT9750.1211572944768428No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9450.11742937772370918No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA9400.11680805826485358No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG9350.11618673880599797No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT9210.11444704432120227No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG9190.11419851653766003No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9120.1133286692952622No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGC9080.11283161372817772No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT9020.11208603037755098No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA9010.11196176648577987No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC8940.111091919243382No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG8890.11047059978452642No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA8870.11022207200098418No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA8840.1098492803256708No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT8810.10947648865035743No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTAC8800.10935222475858633No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC8730.10848237751618847No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTT8710.10823384973264623No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG8670.10773679416556176No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA8570.10649415524785055No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG8430.10475446076305486No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA8380.10413314130419925No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG8190.10177212736054794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAT200.001841608237.06
GGGTGGC250.00549537729.5999987
CGATTCT250.00549537729.59999824
ACGATTC250.00549537729.59999823
GCTCTAG250.00549537729.5999981
GGGACGT250.00549537729.59999818
GCCGAAG250.00549537729.59999836
CAGATGG405.9379505E-527.759
AGTCATA556.257833E-726.9090910
ATAGACC358.867073E-426.4285723
GGCCAAA358.867073E-426.42857219
ATATACA358.867073E-426.4285721
GACGTAT809.786163E-1025.437528
CCGGCAG2800.025.10714316
CGCTCTC2750.024.8909129
CAATTTT451.3227304E-424.6666669
TACAATT451.3227304E-424.6666667
CCAAGCA2200.024.38636436
GGTATCA16100.024.3602491
CCGCTCT2750.024.21818428
Produced by FastQC (version 0.11.5)