FastQCFastQC Report
Fri 10 Feb 2017
ERR1633462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914693
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49170.5375574099725263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35990.39346534848304293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19230.21023447211250113No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG17710.19361687473283387No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA17570.19208630655312767No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG15770.172407572814048No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG14560.15917909068944444No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT11520.1259438959301099No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT10810.11818172873302846No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC10750.11752577094172581No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA10700.11697913944897359No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC10340.11304339270115765No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC10060.10998225634174526No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG9770.10681179368378242No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.1018921102490125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.024.2349991
TGCGGTA502.7015177E-422.236
GTATCAA24700.021.7955471
GCCGGCA1750.019.0285715
TGCTCGC1950.018.9743610
TAAGCCG500.007033810518.55
CTTAGAC609.234849E-418.53
TTCGCCG1552.0008883E-1117.90322724
GCCGCTC1451.546141E-1017.8620727
ATTAGAC1054.7946196E-717.619053
TTGCCGA1054.7946196E-717.6190510
CCGCTCT1502.5102054E-1017.26666628
CCGGCAG2050.017.14634116
TAGGACC2050.017.1463414
GCTTCGC1757.2759576E-1216.91428622
GTATTAT2200.016.8181821
CGAACTA1901.8189894E-1216.55263129
GCGAACT1901.8189894E-1216.55263128
CGCTCTC1606.2937033E-1016.187529
GCGACAA1151.2414403E-616.08695616
Produced by FastQC (version 0.11.5)