##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633462.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	914693
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.837005421491146	33.0	33.0	33.0	27.0	33.0
2	31.94751025753996	33.0	33.0	33.0	33.0	33.0
3	31.678022024876107	33.0	33.0	33.0	27.0	33.0
4	31.957535479117038	33.0	33.0	33.0	33.0	33.0
5	32.06607134852896	33.0	33.0	33.0	33.0	33.0
6	35.22560902947765	37.0	37.0	37.0	33.0	37.0
7	35.36911838179586	37.0	37.0	37.0	33.0	37.0
8	35.51447753508554	37.0	37.0	37.0	33.0	37.0
9	35.63104998070391	37.0	37.0	37.0	33.0	37.0
10	35.64391440625434	37.0	37.0	37.0	33.0	37.0
11	35.659616942515136	37.0	37.0	37.0	33.0	37.0
12	35.61794613055965	37.0	37.0	37.0	33.0	37.0
13	35.645232881414856	37.0	37.0	37.0	33.0	37.0
14	35.619414382749184	37.0	37.0	37.0	33.0	37.0
15	35.644521167211295	37.0	37.0	37.0	33.0	37.0
16	35.61941110296023	37.0	37.0	37.0	33.0	37.0
17	35.60195387960769	37.0	37.0	37.0	33.0	37.0
18	35.60041347206112	37.0	37.0	37.0	33.0	37.0
19	35.58311258531551	37.0	37.0	37.0	33.0	37.0
20	35.58639456079799	37.0	37.0	37.0	33.0	37.0
21	35.58108567574038	37.0	37.0	37.0	33.0	37.0
22	35.485898547381474	37.0	37.0	37.0	33.0	37.0
23	35.536794312408645	37.0	37.0	37.0	33.0	37.0
24	35.522085552201666	37.0	37.0	37.0	33.0	37.0
25	35.547076450787316	37.0	37.0	37.0	33.0	37.0
26	35.43429434793969	37.0	37.0	37.0	33.0	37.0
27	35.44194828210121	37.0	37.0	37.0	33.0	37.0
28	35.47797020421059	37.0	37.0	37.0	33.0	37.0
29	35.474238897641065	37.0	37.0	37.0	33.0	37.0
30	35.47816480502201	37.0	37.0	37.0	33.0	37.0
31	35.47247109139351	37.0	37.0	37.0	33.0	37.0
32	35.456242695636675	37.0	37.0	37.0	33.0	37.0
33	35.46164450804805	37.0	37.0	37.0	33.0	37.0
34	35.43930695872823	37.0	37.0	37.0	33.0	37.0
35	35.37092991856284	37.0	37.0	37.0	33.0	37.0
36	35.40221363889305	37.0	37.0	37.0	33.0	37.0
37	35.38928033777453	37.0	37.0	37.0	33.0	37.0
38	35.38673194175532	37.0	37.0	37.0	33.0	37.0
39	35.279597635490816	37.0	37.0	37.0	33.0	37.0
40	35.07602550801198	37.0	37.0	37.0	33.0	37.0
41	35.24585407344322	37.0	37.0	37.0	33.0	37.0
42	35.28494041170097	37.0	37.0	37.0	33.0	37.0
43	34.95898405257283	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	25.0
16	43.0
17	53.0
18	35.0
19	48.0
20	59.0
21	154.0
22	382.0
23	930.0
24	1977.0
25	3594.0
26	5897.0
27	9016.0
28	12744.0
29	17164.0
30	22613.0
31	28715.0
32	37051.0
33	48800.0
34	70263.0
35	143089.0
36	512038.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92346503143678	20.918056659447487	13.541701969950573	23.616776339165163
2	16.186195805587232	22.247027144626667	38.92978299823	22.636994051556094
3	17.796244204339597	27.358906212248264	30.04024301049642	24.804606572915723
4	11.959641103627119	18.16215932558793	40.17336964424129	29.704829926543656
5	13.769975281323898	36.995144819081375	35.4709175646911	13.763962334903624
6	29.050184050823606	40.57437850732432	17.397640519824684	12.977796922027391
7	27.053776512993977	32.06310751257526	23.47804126630465	17.405074708126115
8	24.533258699913524	35.57280967494012	20.82939303132308	19.064538593823283
9	26.003697415416976	15.1295571300972	20.635776156590243	38.23096929789558
10	15.025369167578631	28.12615817547527	34.20710555344799	22.641367103498116
11	33.78237288357952	24.351339739125585	24.027187263923526	17.839100113371373
12	22.698544757639997	25.860261311718798	30.79623436497273	20.64495956566848
13	27.91319054589901	22.671213183002383	26.42668086450864	22.98891540658997
14	21.99863779432006	21.47387156127794	28.194159133173645	28.33333151122836
15	23.321486006780418	29.28731279237952	25.318658828699903	22.072542372140163
16	23.221889748800965	28.73379374281863	25.184187481482855	22.86012902689755
17	22.621797696057584	27.45172423971759	26.41028191972607	23.516196144498753
18	22.606710666857623	26.83916898893946	28.5906856180161	21.96343472618682
19	25.25207911288268	26.38765137592613	27.799709848003644	20.56055966318754
20	24.46296188994559	26.683379013505075	27.773799515247195	21.07985958130214
21	22.772777314355746	27.307632178228104	27.729850343229916	22.189740164186237
22	23.197837963119866	26.4174974554304	27.929917469577227	22.454747111872507
23	22.20056346774273	26.780023461423667	28.700339895462196	22.31907317537141
24	22.510284871536133	27.796211406450034	27.16332146414152	22.530182257872315
25	22.78884828024266	27.409633614775668	28.217554961063442	21.583963143918233
26	23.60890484566953	26.90476476806972	27.322828533726618	22.16350185253413
27	22.895769400224992	26.91700931350737	27.095648485338796	23.091572800928837
28	22.377562745095897	26.707430799186177	28.44232983088315	22.47267662483478
29	23.59687895282898	27.254936902326794	27.574388346691187	21.573795798153043
30	23.35756368530206	26.65943655412253	27.584446366157827	22.39855339441758
31	22.808854992877393	26.2326266845816	28.01541063504367	22.943107687497335
32	21.84711154452915	26.822442065261242	28.058375870373993	23.272070519835616
33	22.228332347574543	26.532618047804018	28.557450423256764	22.681599181364675
34	23.083701307433206	26.58301747143577	28.239420220773525	22.0938610003575
35	22.900907736256865	27.309381399004913	27.168350473874842	22.621360390863384
36	23.041282703595634	26.79992084775985	27.130523574576387	23.02827287406813
37	23.02608634809712	26.495556432595418	27.8626817959687	22.61567542333876
38	22.75900220073839	26.51064346179538	27.430842916694452	23.29951142077178
39	22.458901511217423	26.666214784632658	28.07127637360295	22.80360733054697
40	22.982027849781293	27.29735550616436	27.74012701529366	21.980489628760687
41	22.70729086152403	26.536444468253283	27.818185992458673	22.938078677764015
42	21.914237891839118	28.29408337004875	27.363825895683032	22.4278528424291
43	23.405011298872953	26.118490028894943	27.33157463761065	23.144924034621454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	61.5
2	82.0
3	330.0
4	578.0
5	578.0
6	734.0
7	890.0
8	794.0
9	698.0
10	954.0
11	1210.0
12	1210.0
13	1922.5
14	2635.0
15	4702.5
16	6770.0
17	7717.5
18	8665.0
19	8665.0
20	8292.0
21	7919.0
22	8925.5
23	9932.0
24	12636.5
25	15341.0
26	15341.0
27	18417.0
28	21493.0
29	24792.5
30	28092.0
31	32416.0
32	36740.0
33	36740.0
34	45377.0
35	54014.0
36	57175.5
37	60337.0
38	61959.0
39	63581.0
40	63581.0
41	63716.5
42	63852.0
43	65415.5
44	66979.0
45	65594.0
46	64209.0
47	64209.0
48	61731.5
49	59254.0
50	60416.0
51	61578.0
52	62722.5
53	63867.0
54	63867.0
55	61521.0
56	59175.0
57	50284.5
58	41394.0
59	38186.0
60	34978.0
61	34978.0
62	31486.5
63	27995.0
64	21838.0
65	15681.0
66	13451.0
67	11221.0
68	11221.0
69	9621.5
70	8022.0
71	8032.5
72	8043.0
73	7099.5
74	6156.0
75	6156.0
76	3753.0
77	1350.0
78	1043.5
79	737.0
80	602.0
81	467.0
82	467.0
83	374.5
84	282.0
85	251.0
86	220.0
87	175.0
88	130.0
89	130.0
90	98.0
91	66.0
92	42.0
93	18.0
94	9.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	914693.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.84003670700619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.5575921224418	48.89219158094226
2	7.534478239106425	8.414510828796843
3	1.6949301578159301	2.8393488668475997
4	0.7716854619258993	1.7236377808082095
5	0.4233777601471762	1.182071483377419
6	0.2938728089298139	0.9845921062699093
7	0.22342441168999913	0.873321914500757
8	0.17551482125271825	0.7840603249100334
9	0.1282194322219253	0.6443800021641422
>10	0.9359024188055339	10.402324977847211
>50	0.14131961389404862	5.583287805924324
>100	0.11015576655852748	12.52940696108675
>500	0.006946760049097159	2.551432795769972
>1k	0.0025802251610932306	2.5954325707545807
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4917	0.5375574099725263	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3599	0.39346534848304293	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1923	0.21023447211250113	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1771	0.19361687473283387	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1757	0.19208630655312767	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1577	0.172407572814048	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1456	0.15917909068944444	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	1152	0.1259438959301099	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	1081	0.11818172873302846	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1075	0.11752577094172581	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	1070	0.11697913944897359	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	1034	0.11304339270115765	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	1006	0.10998225634174526	No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	977	0.10681179368378242	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	932	0.1018921102490125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.093262985504426E-4	0.0	0.0
2	0.0	0.0	1.093262985504426E-4	0.0	0.0
3	0.0	0.0	2.186525971008852E-4	0.0	0.0
4	0.0	0.0	4.373051942017704E-4	0.0	0.0
5	0.0	0.0	4.373051942017704E-4	0.0	0.0
6	0.0	0.0	7.652840898530982E-4	0.0	0.0
7	0.0	0.0	0.0021865259710088522	1.093262985504426E-4	0.0
8	0.0	0.0	0.002405178568109737	2.186525971008852E-4	0.0
9	0.0	0.0	0.0029518100608619505	5.466314927522131E-4	0.0
10	2.186525971008852E-4	0.0	0.0032797889565132784	6.559577913026557E-4	0.0
11	2.186525971008852E-4	0.0	0.00546631492752213	7.652840898530982E-4	0.0
12	2.186525971008852E-4	0.0	0.006996883107228327	9.839366869539834E-4	0.0
13	2.186525971008852E-4	0.0	0.0074341883014300975	0.0010932629855044261	0.0
14	2.186525971008852E-4	0.0	0.008199472391283196	0.0012025892840548686	0.0
15	2.186525971008852E-4	0.0	0.008855430182585852	0.0012025892840548686	0.0
16	2.186525971008852E-4	0.0	0.009292735376787622	0.0013119155826053113	0.0
17	2.186525971008852E-4	0.0	0.009948693168090278	0.0013119155826053113	0.0
18	2.186525971008852E-4	0.0	0.009948693168090278	0.001421241881155754	0.0
19	2.186525971008852E-4	0.0	0.010167345765191162	0.001421241881155754	0.0
20	2.186525971008852E-4	0.0	0.010385998362292048	0.001421241881155754	0.0
21	2.186525971008852E-4	0.0	0.010604650959392933	0.001421241881155754	0.0
22	2.186525971008852E-4	0.0	0.010823303556493817	0.0017492207768070817	0.0
23	2.186525971008852E-4	0.0	0.010823303556493817	0.002405178568109737	0.0
24	3.279788956513278E-4	0.0	0.010823303556493817	0.002842483762311508	0.0
25	3.279788956513278E-4	0.0	0.010823303556493817	0.0029518100608619505	0.0
26	3.279788956513278E-4	0.0	0.010823303556493817	0.0037170941507150487	0.0
27	3.279788956513278E-4	0.0	0.010823303556493817	0.005138336031870802	0.0
28	3.279788956513278E-4	0.0	0.010823303556493817	0.012025892840548686	0.0
29	3.279788956513278E-4	0.0	0.010823303556493817	0.027987532428913307	0.0
30	3.279788956513278E-4	0.0	0.010823303556493817	0.052695275901313336	0.0
31	4.373051942017704E-4	0.0	0.010823303556493817	0.11413665568666208	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1000	0.0	24.234999	1
TGCGGTA	50	2.7015177E-4	22.2	36
GTATCAA	2470	0.0	21.795547	1
GCCGGCA	175	0.0	19.02857	15
TGCTCGC	195	0.0	18.97436	10
TAAGCCG	50	0.0070338105	18.5	5
CTTAGAC	60	9.234849E-4	18.5	3
TTCGCCG	155	2.0008883E-11	17.903227	24
GCCGCTC	145	1.546141E-10	17.86207	27
ATTAGAC	105	4.7946196E-7	17.61905	3
TTGCCGA	105	4.7946196E-7	17.61905	10
CCGCTCT	150	2.5102054E-10	17.266666	28
CCGGCAG	205	0.0	17.146341	16
TAGGACC	205	0.0	17.146341	4
GCTTCGC	175	7.2759576E-12	16.914286	22
GTATTAT	220	0.0	16.818182	1
CGAACTA	190	1.8189894E-12	16.552631	29
GCGAACT	190	1.8189894E-12	16.552631	28
CGCTCTC	160	6.2937033E-10	16.1875	29
GCGACAA	115	1.2414403E-6	16.086956	16
>>END_MODULE