FastQCFastQC Report
Fri 10 Feb 2017
ERR1633461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787448
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35740.45387123975170424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24580.312147595777753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13960.17728154747996058No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT10730.13626296593552842No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10680.13562800337292114No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC9930.1261035649338115No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC9720.1234367221708608No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA8320.10565777041785615No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA7980.10134002499212647No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA7980.10134002499212647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6200.025.3629021
CGAGTCG451.3226956E-424.66666421
GTATCAA17250.023.1652161
CCGTATC400.001930650223.1259
GATACTA450.00382475520.5555535
TCGCCGT555.141117E-420.18181825
GAGTCGC555.141117E-420.18181822
CCGAGTT555.141117E-420.18181813
GGACCGT555.141117E-420.1818186
ACTGACG609.2332956E-418.58
CTACACT1205.16593E-918.54
CGAGACT500.00703296118.54
CGCCGTC609.2332956E-418.526
ACCGTTA1258.565621E-917.768
GTCGCCC953.6034671E-617.52631637
GTCCGGC752.0662247E-417.26666615
CGGTTCA650.001579336117.07692333
AGTCGCC650.001579336117.07692323
CCGATCT1201.0399344E-716.9583329
GCGAACT1554.0017767E-1016.70967728
Produced by FastQC (version 0.11.5)