##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633461.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	787448
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.673834970690127	33.0	33.0	33.0	27.0	33.0
2	31.84884716197133	33.0	33.0	33.0	33.0	33.0
3	31.562545082341945	33.0	33.0	33.0	27.0	33.0
4	31.860306966301266	33.0	33.0	33.0	27.0	33.0
5	31.963605215836473	33.0	33.0	33.0	33.0	33.0
6	35.067690818949316	37.0	37.0	37.0	33.0	37.0
7	35.2094766892544	37.0	37.0	37.0	33.0	37.0
8	35.331082179394706	37.0	37.0	37.0	33.0	37.0
9	35.458733783056154	37.0	37.0	37.0	33.0	37.0
10	35.483178571791406	37.0	37.0	37.0	33.0	37.0
11	35.4862352815678	37.0	37.0	37.0	33.0	37.0
12	35.4883052595219	37.0	37.0	37.0	33.0	37.0
13	35.48806016397273	37.0	37.0	37.0	33.0	37.0
14	35.465663764464445	37.0	37.0	37.0	33.0	37.0
15	35.47309155652183	37.0	37.0	37.0	33.0	37.0
16	35.4619898710772	37.0	37.0	37.0	33.0	37.0
17	35.45276894474302	37.0	37.0	37.0	33.0	37.0
18	35.43124117402038	37.0	37.0	37.0	33.0	37.0
19	35.42817557476811	37.0	37.0	37.0	33.0	37.0
20	35.435878940577666	37.0	37.0	37.0	33.0	37.0
21	35.4267392894515	37.0	37.0	37.0	33.0	37.0
22	35.33342646117585	37.0	37.0	37.0	33.0	37.0
23	35.38011398847924	37.0	37.0	37.0	33.0	37.0
24	35.36405959504628	37.0	37.0	37.0	33.0	37.0
25	35.3810943706759	37.0	37.0	37.0	33.0	37.0
26	35.26773704422387	37.0	37.0	37.0	33.0	37.0
27	35.28128714530991	37.0	37.0	37.0	33.0	37.0
28	35.30275141977629	37.0	37.0	37.0	33.0	37.0
29	35.30541826253924	37.0	37.0	37.0	33.0	37.0
30	35.31511922057076	37.0	37.0	37.0	33.0	37.0
31	35.3031400168646	37.0	37.0	37.0	33.0	37.0
32	35.29765648017393	37.0	37.0	37.0	33.0	37.0
33	35.28511723948756	37.0	37.0	37.0	33.0	37.0
34	35.26856630533064	37.0	37.0	37.0	33.0	37.0
35	35.20958717274029	37.0	37.0	37.0	33.0	37.0
36	35.24028126301673	37.0	37.0	37.0	33.0	37.0
37	35.23217406101736	37.0	37.0	37.0	33.0	37.0
38	35.21939099470695	37.0	37.0	37.0	33.0	37.0
39	35.11190961180929	37.0	37.0	37.0	33.0	37.0
40	34.90443432455223	37.0	37.0	37.0	27.0	37.0
41	35.08509133301501	37.0	37.0	37.0	27.0	37.0
42	35.130974997714134	37.0	37.0	37.0	33.0	37.0
43	34.78189797929514	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	29.0
16	52.0
17	22.0
18	33.0
19	42.0
20	67.0
21	167.0
22	483.0
23	1028.0
24	2124.0
25	3829.0
26	6195.0
27	9014.0
28	12478.0
29	16454.0
30	21081.0
31	27080.0
32	34187.0
33	45259.0
34	65018.0
35	129731.0
36	413069.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.508198636608384	21.377665572837827	14.247416972295312	23.866718818258477
2	15.465656144913694	22.34039581026303	39.652650079751304	22.54129796507198
3	17.612083591551443	27.81491603255072	31.136278205037033	23.436722170860804
4	12.615817171419575	18.463187410470276	39.84402779612114	29.07696762198901
5	13.24544604850098	38.121501356280035	35.722231817212055	12.91082077800693
6	28.284407351342566	40.675066798061586	17.92181832959129	13.118707521004561
7	25.050797005008583	33.18542938708334	24.312589529721325	17.45118407818675
8	23.68194471253975	36.88840405969664	21.004942548587337	18.424708679176277
9	25.22223689691256	16.267232883949163	20.763910759821602	37.746619459316676
10	14.32437443488332	29.07125295892554	34.387667503124014	22.21670510306712
11	32.420680476679095	24.635531489063407	24.980443153071693	17.963344881185805
12	22.018977761071206	26.67071349473235	30.817653991120686	20.492654753075758
13	27.083566152939625	22.97116761995713	27.215892350986987	22.729373876116263
14	21.351632107770925	22.058472432465383	28.521629364732654	28.068266095031035
15	23.314936351352724	29.48270869949508	25.369929188975014	21.83242576017718
16	22.19664028608873	29.68538874947933	25.48574636039459	22.632224604037347
17	21.753817394926394	28.558584185876402	26.925841452387967	22.761756966809237
18	21.803979437372373	28.08477512165883	28.515533724131625	21.595711716837176
19	23.912563115278722	27.31024778779043	28.723674452154302	20.053514644776545
20	23.608797025327384	26.67147544980748	28.790599506253113	20.929128018612023
21	21.934527740244437	28.127698590891082	28.036009996850588	21.9017636720139
22	21.61945931667869	27.97543456837785	28.166304314697605	22.23880180024586
23	21.625427964767198	27.8236785159147	28.580807875567654	21.970085643750444
24	22.5656805274761	28.210497709055076	27.48194166471945	21.741880098749377
25	22.494818705489124	27.454003311964726	28.34663368247808	21.70454430006807
26	22.284519104753585	27.51534069551259	28.137477014355234	22.06266318537859
27	22.003865652081153	27.589885300362692	27.93466997185846	22.471579075697697
28	21.37715760278774	27.78088203919497	28.779424165151223	22.06253619286607
29	22.79833081041542	27.87726935619876	27.712687059970943	21.61171277341488
30	21.97643526937652	28.05124909835316	28.20516402352917	21.76715160874115
31	22.397796425922728	27.490323170545867	28.419781369690444	21.692099033840964
32	21.210162448822018	27.657318324511586	28.575474190041756	22.55704503662464
33	21.443828671861507	27.49946663144741	28.832380042872675	22.22432465381841
34	21.851093659517833	27.57566213896029	28.26459651938922	22.308647682132662
35	22.106729587223537	28.18560717660087	27.835234834554157	21.87242840162144
36	22.59006308988022	27.858982434395667	27.633824709695116	21.917129766028996
37	22.677052960957422	27.031626215318344	27.763865042517093	22.527455781207138
38	22.631081671424653	27.781643994270098	27.824186485964788	21.76308784834046
39	22.04450325608802	27.485497455070053	28.223450945332264	22.246548343509666
40	22.21454623035426	27.640047342808664	28.31285367414737	21.8325527526897
41	21.784041612906503	27.50213347421036	28.54194816673609	22.17187674614705
42	22.092506425821135	27.61883959321758	28.028136461074254	22.260517519887028
43	22.06494905060398	27.222495961638103	28.174939805549066	22.537615182208857
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	59.0
1	66.5
2	74.0
3	223.0
4	372.0
5	372.0
6	463.5
7	555.0
8	501.0
9	447.0
10	683.0
11	919.0
12	919.0
13	1407.5
14	1896.0
15	3723.0
16	5550.0
17	6448.0
18	7346.0
19	7346.0
20	7249.0
21	7152.0
22	8711.5
23	10271.0
24	12557.5
25	14844.0
26	14844.0
27	18175.0
28	21506.0
29	26692.0
30	31878.0
31	34681.5
32	37485.0
33	37485.0
34	42008.0
35	46531.0
36	48670.5
37	50810.0
38	54399.5
39	57989.0
40	57989.0
41	61393.5
42	64798.0
43	61122.0
44	57446.0
45	57442.5
46	57439.0
47	57439.0
48	55774.0
49	54109.0
50	54812.5
51	55516.0
52	54805.0
53	54094.0
54	54094.0
55	47449.0
56	40804.0
57	35852.0
58	30900.0
59	27242.0
60	23584.0
61	23584.0
62	20780.5
63	17977.0
64	14891.5
65	11806.0
66	10065.5
67	8325.0
68	8325.0
69	6870.0
70	5415.0
71	4644.5
72	3874.0
73	3177.5
74	2481.0
75	2481.0
76	1899.0
77	1317.0
78	1067.5
79	818.0
80	619.5
81	421.0
82	421.0
83	339.0
84	257.0
85	230.0
86	203.0
87	154.5
88	106.0
89	106.0
90	82.0
91	58.0
92	35.5
93	13.0
94	7.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	787448.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.691601063193474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.02353015458395	48.464797244798774
2	7.433719798127426	8.279915148257516
3	1.9152480744438982	3.199896950969371
4	0.864078572447783	1.9248767657606258
5	0.520896503443778	1.4504780132501636
6	0.33592237083969484	1.1224832799003854
7	0.24530301572191726	0.9562922383828306
8	0.17291725994385662	0.770403124618703
9	0.15479978970858066	0.7758943319804865
>10	1.0554979003718246	11.70393902574616
>50	0.14922306054605328	5.741307271807715
>100	0.122154269130617	12.28618385386484
>500	0.005552466778497283	2.090623857811498
>1k	0.001156763912186934	1.2329088928508707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3574	0.45387123975170424	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2458	0.312147595777753	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1396	0.17728154747996058	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1073	0.13626296593552842	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1068	0.13562800337292114	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	993	0.1261035649338115	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	972	0.1234367221708608	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	832	0.10565777041785615	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	798	0.10134002499212647	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	798	0.10134002499212647	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.539850250429235E-4	0.0	0.0
2	0.0	0.0	3.809775375643852E-4	1.2699251252146175E-4	0.0
3	0.0	0.0	5.07970050085847E-4	2.539850250429235E-4	0.0
4	0.0	0.0	5.07970050085847E-4	2.539850250429235E-4	0.0
5	0.0	0.0	6.349625626073087E-4	2.539850250429235E-4	0.0
6	0.0	0.0	8.889475876502321E-4	3.809775375643852E-4	0.0
7	0.0	0.0	0.001904887687821926	3.809775375643852E-4	0.0
8	0.0	0.0	0.0022858652253863114	3.809775375643852E-4	0.0
9	0.0	0.0	0.00292082778799362	7.619550751287704E-4	0.0
10	0.0	0.0	0.0031748128130365434	8.889475876502321E-4	0.0
11	0.0	0.0	0.004190752913208237	8.889475876502321E-4	0.0
12	0.0	0.0	0.0049527079883370075	8.889475876502321E-4	0.0
13	0.0	0.0	0.00507970050085847	8.889475876502321E-4	0.0
14	0.0	0.0	0.0055876705509443165	0.001015940100171694	0.0
15	0.0	0.0	0.005714663063465778	0.001015940100171694	0.0
16	0.0	0.0	0.006222633113551625	0.0011429326126931557	0.0
17	0.0	0.0	0.006349625626073087	0.0011429326126931557	0.0
18	0.0	0.0	0.00660361065111601	0.0013969176377360791	0.0
19	0.0	0.0	0.0067306031636374716	0.0013969176377360791	0.0
20	0.0	0.0	0.007111580701201857	0.0013969176377360791	0.0
21	0.0	0.0	0.007619550751287704	0.0016509026627790026	0.0
22	0.0	0.0	0.007619550751287704	0.0022858652253863114	0.0
23	0.0	0.0	0.007619550751287704	0.0027938352754721583	0.0
24	0.0	0.0	0.007619550751287704	0.0031748128130365434	0.0
25	0.0	0.0	0.007619550751287704	0.003301805325558005	0.0
26	0.0	0.0	0.007619550751287704	0.003936767888165314	0.0
27	0.0	0.0	0.007619550751287704	0.004571730450772623	0.0
28	0.0	0.0	0.007619550751287704	0.0102863935142384	0.0
29	0.0	0.0	0.007619550751287704	0.021842712153691417	0.0
30	0.0	0.0	0.007619550751287704	0.03746279119383121	0.0
31	0.0	0.0	0.007619550751287704	0.08191017057634283	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	620	0.0	25.362902	1
CGAGTCG	45	1.3226956E-4	24.666664	21
GTATCAA	1725	0.0	23.165216	1
CCGTATC	40	0.0019306502	23.125	9
GATACTA	45	0.003824755	20.555553	5
TCGCCGT	55	5.141117E-4	20.181818	25
GAGTCGC	55	5.141117E-4	20.181818	22
CCGAGTT	55	5.141117E-4	20.181818	13
GGACCGT	55	5.141117E-4	20.181818	6
ACTGACG	60	9.2332956E-4	18.5	8
CTACACT	120	5.16593E-9	18.5	4
CGAGACT	50	0.007032961	18.5	4
CGCCGTC	60	9.2332956E-4	18.5	26
ACCGTTA	125	8.565621E-9	17.76	8
GTCGCCC	95	3.6034671E-6	17.526316	37
GTCCGGC	75	2.0662247E-4	17.266666	15
CGGTTCA	65	0.0015793361	17.076923	33
AGTCGCC	65	0.0015793361	17.076923	23
CCGATCT	120	1.0399344E-7	16.958332	9
GCGAACT	155	4.0017767E-10	16.709677	28
>>END_MODULE