FastQCFastQC Report
Fri 10 Feb 2017
ERR1633460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872867
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49220.5638888857065281No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35210.4033833333142392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19720.22592216225381417No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10500.12029324055096596No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9440.10814935150486844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22250.025.1101111
GGTATCA7400.024.01
TCGATTC450.003825082220.55555537
TCGGCGT555.141732E-420.18181811
GTCAACC2250.018.91111215
ATTACCC2550.018.8627473
GTATTAG3050.018.803281
CGGCGTA609.234388E-418.512
GCGAACT1409.458745E-1118.528
CAACCCA2500.018.517
CATCGCT701.2187706E-418.59
ATCGGCG609.234388E-418.510
CTCGTCT1700.018.49999813
CACGATA500.0070335618.49999821
TGACTCG500.0070335618.49999810
TCAACCC2450.018.12244816
TACCCTC2850.017.5263165
ATCAACG32000.017.2281253
TTAACGG2050.017.14634135
GCGCATA650.001579522217.07692311
Produced by FastQC (version 0.11.5)